GRP78 Binding Chimeric Peptide Motif
- Known as:
- GRP78 Binding Chimeric Peptide Motif
- Catalog number:
- SP2462a
- Product Quantity:
- 1mg
- Category:
- -
- Supplier:
- Abgen
- Gene target:
- GRP78 Binding Chimeric Peptide Motif
Related genes to: GRP78 Binding Chimeric Peptide Motif
- Gene:
- HSPA5 NIH gene
- Name:
- heat shock protein family A (Hsp70) member 5
- Previous symbol:
- GRP78
- Synonyms:
- BiP
- Chromosome:
- 9q33.3
- Locus Type:
- gene with protein product
- Date approved:
- 1991-07-26
- Date modifiied:
- 2015-11-19
Related products to: GRP78 Binding Chimeric Peptide Motif
guanine nucleotide binding protein alpha inhibiting activity polypeptide 1 (GNAI1) polyclonal antibodyα - Calcitonin Gene Related Peptide, α - CGRP, rat&_945;2&_946;1 Integrin Ligand Peptide&_946;_catenin peptide((Cys31,Nva34)_Neuropeptide Y (27_36))2 Salt _ Binding (Disulfide_bond) Synonym SumFormula C116H186N36O28S2((Cys31,Nva34)_Neuropeptide Y (27_36))2 Salt _ Binding (Disulfide_bond) Synonym SumFormula C116H186N36O28S2(1_Adamantaneacetyl1,D_Tyr(Et)2,Val4,Abu6, Arg8·9)_Vasopressin Salt _ Binding _ Synonym SumFormula C62H94N16O11(1_Adamantaneacetyl1,D_Tyr(Et)2,Val4,Abu6, Arg8·9)_Vasopressin Salt _ Binding _ Synonym SumFormula C62H94N16O11(1_Adamantaneacetyl_D_Arg0,Hyp3,b_(2_thienyl)_Ala5·8,D_Phe7)_Bradykinin Salt _ Binding _ Synonym SumFormula C68H99N19O14S2(1_Adamantaneacetyl_D_Arg0,Hyp3,b_(2_thienyl)_Ala5·8,D_Phe7)_Bradykinin Salt _ Binding _ Synonym SumFormula C68H99N19O14S2(1_Adamantanecarbonyl_D_Arg0,Hyp3,b_(2_thienyl)_Ala5·8,D_Phe7)_Bradykinin Salt _ Binding _ Synonym SumFormula C67H97N19O14S2(1_Adamantanecarbonyl_D_Arg0,Hyp3,b_(2_thienyl)_Ala5·8,D_Phe7)_Bradykinin Salt _ Binding _ Synonym SumFormula C67H97N19O14S2(2_Furoyl)_PAR_2 (2_6)_Orn amide (mouse, rat) Salt Trifluoroacetate Binding _ Synonym (2_Furoyl)_LIGRLOamide SumFormula C36H63N11O8(2_Furoyl)_PAR_2 (2_6)_Orn amide (mouse, rat) Salt Trifluoroacetate Binding _ Synonym (2_Furoyl)_LIGRLOamide SumFormula C36H63N11O8(3,4_Dehydro_Pro2·3)_Bradykinin Salt _ Binding _ Synonym SumFormula C50H69N15O11 Related articles to: GRP78 Binding Chimeric Peptide Motif
- Intervertebral disc degeneration (IVDD) is a common and frequent disease in orthopedics, which seriously affects the quality of life of patients. Endoplasmic reticulum stress (ERS)-regulated autophagy and apoptosis play an important role in nucleus pulposus (NP) cells in IVDD. Hypoxia and serum deprivation were used to induce NP cells. Cell counting kit-8 (CCK-8) assay was used to detect cell activity and immunofluorescence (IF) was applied for the appraisement of glucose regulated protein 78 (GRP78) and green fluorescent protein (GFP)-light chain 3 (LC3). Cell apoptosis was detected by flow cytometry and the expression of LC3II/I was detected by western blot. NP cells under hypoxia and serum deprivation were induced by lipopolysaccharide (LPS), and intervened by ERS inhibitor (4-phenylbutyric acid, 4-PBA) and activator (Thapsigargin, TP). Then, above functional experiments were conducted again and western blot was employed for the evaluation of autophagy-, apoptosis and ERS-related proteins. Finally, NP cells under hypoxia and serum deprivation were stimulated by LPS and intervened using apoptosis inhibitor z-Val-Ala-DL-Asp-fluoromethyl ketone (Z-VAD-FMK) and autophagy inhibitor 3-methyladenine (3-MA). CCK-8 assay, IF, flow cytometry and western blot were performed again. Besides, the levels of inflammatory cytokines were measured with enzyme-linked immunosorbent assay (ELISA) and the protein expressions of programmed death markers were estimated with western blot. It showed that serum deprivation induces autophagy and apoptosis. ERS was significantly activated by LPS in hypoxic and serum deprivation environment, and autophagy and apoptosis were significantly promoted. Overall, ERS affects the occurrence and development of IVDD by regulating autophagy, apoptosis and other programmed death. - Source: PubMed
Publication date: 2024/04/22
Dai JiumingLiu JinShen YuchengZhang BingLi ChaonianLiu Zhidong - Dental fluorosis is a discoloration of the teeth caused by the excessive consumption of fluoride. It represents a distinct manifestation of chronic fluorosis in dental tissues, exerting adverse effects on the human body, particularly on teeth. The transmembrane protein 16a (TMEM16A) is expressed at the junction of the endoplasmic reticulum and the plasma membrane. Alterations in its channel activity can disrupt endoplasmic reticulum calcium homeostasis and intracellular calcium ion concentration, thereby inducing endoplasmic reticulum stress (ERS). This study aims to investigate the influence of calcium supplements and TMEM16A on ERS in dental fluorosis. - Source: PubMed
Yu YangyangXia FeiLiu RongYan YahuiYin Li - Ulcerative colitis (UC), a prevalent form of inflammatory bowel disease (IBD), may result from immune system dysfunction, leading to the sustained overproduction of reactive oxygen species (ROS) and subsequent cellular oxidative stress damage. Recent studies have identified both peroxisome proliferator-activated receptor-γ (PPARγ) and endoplasmic reticulum (ER) stress as critical targets for the treatment of IBD. Oroxyloside (CHO), derived from the root of Scutellariabaicalensis Georgi, has traditionally been used in treating inflammatory diseases. In this study, we investigated the molecular mechanisms by which oroxyloside mitigates dextran sulfate sodium (DSS)-induced colitis. We examined the effects of oroxyloside on ROS-mediated ER stress in colitis, including the protein expressions of GRP78, p-PERK, p-eIF2α, ATF4, and CHOP, which are associated with ER stress. The beneficial impact of oroxyloside was reversed by the PPARγ antagonist GW9662 (1 mg·kg, i.v.) in vivo. Furthermore, oroxyloside decreased pro-inflammatory cytokines and ROS production in both bone marrow-derived macrophages (BMDM) and the mouse macrophage cell line RAW 264.7. However, PPARγ siRNA transfection blocked the anti-inflammatory effect of oroxyloside and even abolished ROS generation and ER stress activation inhibited by oroxyloside in vitro. In conclusion, our study demonstrates that oroxyloside ameliorates DSS-induced colitis by inhibiting ER stress via PPARγ activation, suggesting that oroxyloside might be a promising effective agent for IBD. - Source: PubMed
Tao LeiDou RenjieChen XuemingCao YuDai ZhenHu ZiyanMa ZhiGe XiaomingZhang LingWang Xiaoping - The role of drug repositioning in the treatment of ovarian cancer has received increasing attention. Although promising results have been achieved, there are also major controversies. - Source: PubMed
Publication date: 2024/04/08
Zhu LinZhang HairongZhang XiaoyuChen RuoqingXia Lei - The human glucose-regulated protein GRP78 is a human chaperone that translocactes to the cell surface when cells are under stress. Theoretical studies suggested it could be involved in SARS-CoV-2 virus entry to cells. In this work, we used in vitro surface plasmon resonance-based assays to show that human GRP78 indeed binds to SARS-CoV-2 spike protein. We have designed and synthesised cyclic peptides based on the loop structure of amino acids 480-488 of the SARS-CoV-2 spike protein S1 domain from the Wuhan and Omicron variants and showed that both peptides bind to GRP78. Consistent with the greater infectiousness of the Omicron variant, the Omicron-derived peptide displays slower dissociation from the target protein. Both peptides significantly inhibit the binding of wild-type S1 protein to the human protein GRP78 suggesting that further development of these cyclic peptide motifs may provide a viable route to novel anti-SARS-CoV-2 agents. - Source: PubMed
Publication date: 2024/04/13
Johnson NicholasPattinson CraigBurgoyne KateHijazi KarolinHoussen Wael EMilne Bruce Forbes