Electrophoresis Apelex Slim Blotter for gel 10 x 15cm, Plantinized Titanium Anode, fully equipped, incl 2 electrodes, 4 grids, & 6 support pads, for all types of blottings
- Known as:
- Electrophoresis Apelex Slim Blotter electrophoretic matrix 10 x 15cm, Plantinized Titanium Anode, fully equipped, incl 2 electrodes, 4 grids, & 6 support pads, types blottings
- Catalog number:
- APX504002
- Product Quantity:
- each
- Category:
- -
- Supplier:
- Accu
- Gene target:
- Electrophoresis Apelex Slim Blotter for gel 10 15cm Plantinized Titanium Anode fully equipped incl 2 electrodes 4 grids & 6 support pads all types blottings
Related genes to: Electrophoresis Apelex Slim Blotter for gel 10 x 15cm, Plantinized Titanium Anode, fully equipped, incl 2 electrodes, 4 grids, & 6 support pads, for all types of blottings
- Gene:
- CLEC4E NIH gene
- Name:
- C-type lectin domain family 4 member E
- Previous symbol:
- CLECSF9
- Synonyms:
- mincle
- Chromosome:
- 12p13.31
- Locus Type:
- gene with protein product
- Date approved:
- 2001-02-01
- Date modifiied:
- 2015-12-16
Related products to: Electrophoresis Apelex Slim Blotter for gel 10 x 15cm, Plantinized Titanium Anode, fully equipped, incl 2 electrodes, 4 grids, & 6 support pads, for all types of blottings
"Affordable Gel Doc System with UV, Epi white & white
backlight Source for fluorescencent dye-stained DNA (ex.
EtBr)/protein (ex. SYPRO Ruby) gel imaging"'Enduroä 10.10 Horizontal Gel Tank 10 X 10 cm'Enduroä 15.10 Horizontal Gel Tank 15 X 10 cm'Enduroä 15.15 Horizontal Gel Tank 15 X 15 cm'Enduroä 20.20 Horizontal Gel Tank 20 X 20 cm'Enduroä 7.10 Horizontal Gel Tank 7 X 10 cm'Enduroä 7.7 Horizontal Gel Tank 7 x 7 cm(all-cis)-7,10,13,16,19-Docosapentaenoic Acid C22H34O2 CAS: 24880-45-3(all-cis)-7,10,13,16,19-Docosapentaenoic Acid CAS: 24880-45-3 Formula: C22H34O2 (all-cis)-7,10,13,16,19-Docosapentaenoic Acid N-Hydroxysuccinimidyl Ester CAS: Formula: C26H37NO4(all-cis)-7,10,13,16,19-Docosapentaenoic-d5 Acid CAS: Formula: C22H29D5O2(all-cis)-8,11,14,17-Eicosatetraenoic Acid C20H32O2 CAS: 24880-40-8
(all-cis)-8,11,14,17-Eicosatetraenoic Acid CAS: 24880-40-8
Formula: C20H32O2 (all-cis)-8,11,14,17-Eicosatetraenoic Acid N-Hydroxysuccinimidyl Ester CAS: Formula: C24H35NO4(all-Z)-5,8,11,14-Eicosatetraenylphosphonofluoridic Acid Methyl Ester CAS: 188404-10-6 Formula: C21H36FO2P Related articles to: Electrophoresis Apelex Slim Blotter for gel 10 x 15cm, Plantinized Titanium Anode, fully equipped, incl 2 electrodes, 4 grids, & 6 support pads, for all types of blottings
- Mincle (macrophage-inducible C-type lectin, CLEC4E) is a C-type lectin immune-stimulatory receptor that can be targeted for inducing potent adjuvant effects. Mincle can recognize trehalose dimycolate and related glycolipids. Here, we present a protocol to identify the ligand binding mode of Mincle. We describe steps for preparing labeled Mincle ectodomain, data acquisition, and analysis of nuclear magnetic resonance experiments using non-detergent sulfobetaine-195. This protocol can be applied to other protein-ligand interactions that have aggregation problems for complex formation. For complete details on the use and execution of this protocol, please refer to Furukawa et al.. - Source: PubMed
Publication date: 2024/04/03
Furukawa AtsushiKumeta HiroyukiSaitoh TakashiMaenaka Katsumi - This Review aims to coalesce existing knowledge on the human archaeome, a less-studied yet critical non-bacterial component of the human microbiome, with a focus on its interaction with the immune system. Despite a largely bacteria-centric focus in microbiome research, archaea present unique challenges and opportunities for understanding human health. We examine the archaeal distribution across different human body sites, such as the lower gastrointestinal tract (LGT), upper aerodigestive tract (UAT), urogenital tract (UGT), and skin. Variability in archaeal composition exists between sites; methanogens dominate the LGT, while Nitrososphaeria are prevalent on the skin and UAT. Archaea have yet to be classified as pathogens but show associations with conditions such as refractory sinusitis and vaginosis. In the LGT, methanogenic archaea play critical metabolic roles by converting bacterial end-products into methane, correlating with various health conditions, including obesity and certain cancers. Finally, this work looks at the complex interactions between archaea and the human immune system at the molecular level. Recent research has illuminated the roles of specific archaeal molecules, such as RNA and glycerolipids, in stimulating immune responses via innate immune receptors like Toll-like receptor 8 (TLR8) and 'C-type lectin domain family 4 member E' (CLEC4E; also known as MINCLE). Additionally, metabolic by-products of archaea, specifically methane, have demonstrated immunomodulatory effects through anti-inflammatory and anti-oxidative pathways. Despite these advancements, the mechanistic underpinnings of how archaea influence immune activity remain a fertile area for further investigation. - Source: PubMed
Publication date: 2024/03/31
Kuehnast TorbenKumpitsch ChristinaMohammadzadeh RokhsarehWeichhart ThomasMoissl-Eichinger ChristineHeine Holger - CLEC4E has been reported to promote lung cancer progression. Tumor-associated macrophages (TAMs) play an important role in tumorigenesis. Whether the expression of CLEC4E in TAMs is associated with gastric carcinogenesis remains unclear. - Source: PubMed
Publication date: 2024/02/28
Jiang QinXiao DanWang AoYu QiongYin YingWu JingchongZhang YanJin TianKuang BaichengJia Yegui - Punicalagin (PUN) is a polyphenol derived from the pomegranate peel. It has been reported to have many beneficial effects, including anti-inflammatory, anti-oxidant, and anti-proliferation. However, the role of PUN in macrophage phagocytosis is currently unknown. In this study, we found that pre-treatment with PUN significantly enhanced phagocytosis by macrophages in a time- and dose-dependent manner in vitro. Moreover, KEGG enrichment analysis by RNA-sequencing showed that differentially expressed genes following PUN treatment were significantly enriched in phagocyte-related receptors, such as the C-type lectin receptor signaling pathway. Among the C-type lectin receptor family, Mincle (Clec4e) significantly increased at the mRNA and protein level after PUN treatment, as shown by qRT-PCR and western blotting. Small interfering RNA (siRNA) mediated knockdown of Mincle in macrophages resulted in down regulation of phagocytosis. Furthermore, western blotting showed that PUN treatment enhanced the phosphorylation of nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) in macrophages at the early stage. Mincle-mediated phagocytosis by PUN was inhibited by PDTC (a NF-κB inhibitor) and SB203580 (a p38 MAPK inhibitor). In addition, PUN pre-treatment enhanced phagocytosis by peritoneal and alveolar macrophages in vivo. After intraperitoneal injection of Escherichia coli (E.coli), the bacterial load of peritoneal lavage fluid and peripheral blood in PUN pre-treated mice decreased significantly. Similarly, the number of bacteria in the lung tissue significantly reduced after intranasal administration of Pseudomonas aeruginosa (PAO1). Taken together, our results reveal that PUN enhances bacterial clearance in mice by activating the NF-κB and MAPK pathways and upregulating C-type lectin receptor expression to enhance phagocytosis by macrophages. - Source: PubMed
Publication date: 2024/02/29
Yin ShuangqinDai WeihongKuang TianyinZhou JingLuo LiAo ShengxiangYang XueXiao HongyanQiao LinWang RixingWang FeiYun CaihongCheng ShaowenZhu JunyuLiang Huaping - Ischemic stroke (IS) is one of the major diseases threatening human health and survival and a leading cause of acquired mortality and disability in adults. The aim of this study was to screen diagnostic features of IS and to explore the characteristics of immune cell infiltration in IS pathogenesis. - Source: PubMed
Publication date: 2024/01/12
Li QianTian YuNiu JingyanGuo ErliangLu YaohengDang ChunFeng LinLi LeiWang Lihua