E.coli RecQ DNA helicase
- Known as:
- E.coli Rec. Desoxyribonucleic acid helicase
- Catalog number:
- 01-003
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- B-bridge
- Gene target:
- .coli RecQ DNA helicase
Related genes to: E.coli RecQ DNA helicase
- Gene:
- FCN2 NIH gene
- Name:
- ficolin 2
- Previous symbol:
- -
- Synonyms:
- P35, FCNL, EBP-37, ficolin-2
- Chromosome:
- 9q34.3
- Locus Type:
- gene with protein product
- Date approved:
- 1996-07-11
- Date modifiied:
- 2016-10-05
- Gene:
- RECQL NIH gene
- Name:
- RecQ like helicase
- Previous symbol:
- -
- Synonyms:
- RecQ1, RecQL1
- Chromosome:
- 12p12.1
- Locus Type:
- gene with protein product
- Date approved:
- 1994-03-25
- Date modifiied:
- 2016-02-05
- Gene:
- RECQL4 NIH gene
- Name:
- RecQ like helicase 4
- Previous symbol:
- -
- Synonyms:
- RecQ4
- Chromosome:
- 8q24.3
- Locus Type:
- gene with protein product
- Date approved:
- 1999-05-06
- Date modifiied:
- 2019-04-23
- Gene:
- RECQL5 NIH gene
- Name:
- RecQ like helicase 5
- Previous symbol:
- -
- Synonyms:
- RecQ5, FLJ90603
- Chromosome:
- 17q25
- Locus Type:
- gene with protein product
- Date approved:
- 1999-05-06
- Date modifiied:
- 2016-02-05
Related products to: E.coli RecQ DNA helicase
"Affordable Gel Doc System with UV, Epi white & white
backlight Source for fluorescencent dye-stained DNA (ex.
EtBr)/protein (ex. SYPRO Ruby) gel imaging"(+) Control probe (DNA), biotinylated(-) Control probe (DNA), biotinylated(Des_Met0)_rec TGF a (human) (expressed in E.coli) Salt _ Binding _ Synonym SumFormula(Des_Met0)_rec TGF a (human) (expressed in E.coli) Salt _ Binding _ Synonym SumFormula* Colonisation Medium w_Dulbecco’s Phosphate Buffer USE For the preparation of suspension of enterotoxigenic Escherichia coli used for HeLa cell test for colonization.*B12 Assay Agar Using E.coli Mutant Culture (Harrison et al. Medium) USE For microbiological assay of Vitamin B12 using E.coli mutant 113 _ 3 Davis ATCC 11105.*B12 Culture Agar (E.coli Maintenance Medium ) (E. coli Mutant Culture Agar) USE For propogation cultivation and maintenance of E.coli mutant used in microbiological assay of vitamin B 12.*E.coli Mutant Culture Agar, B12 Culture Agar USE For propogation, cultivation and maintenance of Escherichia coli mutant used in microbiological assay of vitamin B 12.*Letheen Agar USE For determination of the phenol coefficient of quaternary ammonium compounds using Escherichia coli or Staphylococcus aureus ATCC 6538.*Letheen Broth,AOAC USE For determination of bacterial activity of quaternary ammonium compounds using Escherichia coli or Staphylococcus aureus ATCC 6538.-: PSME1, 1-249aa, Human, His tag, E.coli-: PSME1, 1-249aa, Human, His tag, E.coli-: RBM8A, 1-174aa, Human, His tag, E.coli-: RBM8A, 1-174aa, Human, His tag, E.coli Related articles to: E.coli RecQ DNA helicase
- RecQ helicases are a family of proteins involved in maintaining genome integrity with functions in DNA repair, recombination, and replication. The human RecQ helicase family consists of five helicases: BLM, WRN, RECQL, RECQL4, and RECQL5. Inherited mutations in RecQ helicases result in Bloom Syndrome (BLM mutation), Werner Syndrome (WRN mutation), Rothmund-Thomson Syndrome (RECQL4 mutation), and other genetic diseases, including cancer. The RecQ helicase family is evolutionarily conserved, as Drosophila melanogaster have three family members: DmBlm, DmRecQL4, and DmRecQL5 and DmWRNexo, which contains a conserved exonuclease domain. DmBlm has functional similarities to human BLM (hBLM) as mutants demonstrate increased sensitivity to ionizing radiation (IR) and a decrease in DNA double-strand break (DSB) repair. To determine the extent of functional conservation of RecQ helicases, hBLM was expressed in Drosophila using the GAL4 > UASp system to determine if GAL4 > UASp::hBLM can rescue DmBlm mutant sensitivity to IR. hBLM was able to rescue female DmBlm mutant sensitivity to IR, supporting functional conservation. This functional conservation is specific to BLM, as human GAL4 > UASp::RECQL was not able to rescue DmBlm mutant sensitivity to IR. These results demonstrate the conserved role of BLM in maintaining the genome while reinforcing the applicability of using Drosophila as a model system to study Bloom Syndrome. - Source: PubMed
Publication date: 2019/11/26
Cox Rebecca LHofley Carolyn MTatapudy PallaviPatel Romil KDayani YaronBetcher MadisonLaRocque Jeannine R - Five RecQ helicase family members have a role in maintaining genome stability. However, their prognostic roles in breast cancer remain unknown. We aimed to investigate the prognostic values of the RecQ family and clinical outcomes in breast cancer. - Source: PubMed
Publication date: 2018/12/05
Zhu XuanChen HuihuiYang YiXu ChunjingZhou JunZhou JiaojiaoChen Yiding - Heritable loss of function mutations in the human RECQ helicase genes BLM, WRN, and RECQL4 cause Bloom, Werner, and Rothmund-Thomson syndromes, cancer predispositions with additional developmental or progeroid features. In order to better understand RECQ pathogenic and population variation, we systematically analyzed genetic variation in all five human RECQ helicase genes. A total of 3,741 unique base pair-level variants were identified, across 17,605 potential mutation sites. Direct counting of BLM, RECQL4, and WRN pathogenic variants was used to determine aggregate and disease-specific carrier frequencies. The use of biochemical and model organism data, together with computational prediction, identified over 300 potentially pathogenic population variants in RECQL and RECQL5, the two RECQ helicases that are not yet linked to a heritable deficiency syndrome. Despite the presence of these predicted pathogenic variants in the human population, we identified no individuals homozygous for any biochemically verified or predicted pathogenic RECQL or RECQL5 variant. Nor did we find any individual heterozygous for known pathogenic variants in two or more of the disease-associated RECQ helicase genes BLM, RECQL4, or WRN. Several postulated RECQ helicase deficiency syndromes-RECQL or RECQL5 loss of function, or compound haploinsufficiency for the disease-associated RECQ helicases-may remain missing, as they likely incompatible with life. - Source: PubMed
Publication date: 2016/12/09
Fu WenqingLigabue AlessioRogers Kai JAkey Joshua MMonnat Raymond J - Human RecQ helicase family, consisting of RECQL, RECQL4, RECQL5, BLM and WRN, has critical roles in genetic stability and tumorigenesis. Although RECQL5 has been reported to correlate with the susceptibility to malignances including osteosarcoma, the specific effect on tumor genesis and progression is not yet clarified. Here we focused on the relationship between RECQL5 expression and osteosarcoma disease progression, and further investigated the function of RECQL5 on MG-63 cell proliferation and apoptosis. By immunohistochemical analysis, qRT-PCR and western blot, we found that RECQL5 expression was downregulated in osteosarcoma tissues and cells. Patients with advanced tumor stage and low grade expressed lower RECQL5. To construct a stable RECQL5 overexpression osteosarcoma cell line (MG-63-RECQL5), RECQL5 gene was inserted into the human AAVS1 safe harbor by CRISPR/Cas9 system. The overexpression of RECQL5 was verified by qRT-PCR and western blot. Cell proliferation, cell cycle and apoptosis assay revealed that RECQL5 overexpression inhibited proliferation, induced G1-phase arrest and promoted apoptosis in MG-63 cells. Collectively, our results suggested RECQL5 as a tumor suppressor in osteosarcoma and may be a potential therapeutic target for osteosarcoma treatment. - Source: PubMed
Publication date: 2015/10/22
Wu JunlongZhi LiqiangDai XinCai QingchunMa Wei - Genome instability represents a primary hallmark of aging and cancer. RecQL helicases (i.e., RECQL1, WRN, BLM, RECQL4, RECQL5) as well as poly(ADP-ribose) polymerases (PARPs, in particular PARP1) represent two central quality control systems to preserve genome integrity in mammalian cells. Consistently, both enzymatic families have been linked to mechanisms of aging and carcinogenesis in mice and humans. This is in accordance with clinical and epidemiological findings demonstrating that defects in three RecQL helicases, i.e., WRN, BLM, RECQL4, are related to human progeroid and cancer predisposition syndromes, i.e., Werner, Bloom, and Rothmund Thomson syndrome, respectively. Moreover, PARP1 hypomorphy is associated with a higher risk for certain types of cancer. On a molecular level, RecQL helicases and PARP1 are involved in the control of DNA repair, telomere maintenance, and replicative stress. Notably, over the last decade, it became apparent that all five RecQL helicases physically or functionally interact with PARP1 and/or its enzymatic product poly(ADP-ribose) (PAR). Furthermore, a profound body of evidence revealed that the cooperative function of RECQLs and PARP1 represents an important factor for maintaining genome integrity. In this review, we summarize the status quo of this molecular cooperation and discuss open questions that provide a basis for future studies to dissect the cooperative functions of RecQL helicases and PARP1 in aging and carcinogenesis. - Source: PubMed
Publication date: 2014/12/30
Veith SebastianMangerich Aswin