Calpain_9 (EF Hand Region)
- Known as:
- Calpain_9 (EF Hand Region)
- Catalog number:
- AP05863PU-N
- Product Quantity:
- 0.15 mg
- Category:
- -
- Supplier:
- ACR
- Gene target:
- Calpain_9 ( Hand Region)
Related genes to: Calpain_9 (EF Hand Region)
- Gene:
- HAND1 NIH gene
- Name:
- heart and neural crest derivatives expressed 1
- Previous symbol:
- -
- Synonyms:
- eHand, Thing1, Hxt, bHLHa27
- Chromosome:
- 5q33.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-05-17
- Date modifiied:
- 2016-10-05
- Gene:
- HAND2 NIH gene
- Name:
- heart and neural crest derivatives expressed 2
- Previous symbol:
- -
- Synonyms:
- dHand, Thing2, Hed, bHLHa26
- Chromosome:
- 4q34.1
- Locus Type:
- gene with protein product
- Date approved:
- 1999-05-17
- Date modifiied:
- 2015-08-25
Related products to: Calpain_9 (EF Hand Region)
Related articles to: Calpain_9 (EF Hand Region)
- Transcription factors HAND1 and HAND2 (HAND1/2) play significant roles in cardiac organogenesis. Abnormal expression and deficiency of HAND1/2 result in severe cardiac defects. However, the function and mechanism of HAND1/2 in regulating human early cardiac lineage commitment and differentiation are still unclear. - Source: PubMed
Publication date: 2024/02/05
Guo HuixinHang ChengwenLin BowenLin ZheyiXiong HuiZhang MingshuaiLu RenhongLiu JunyangShi DanXie DuanyangLiu YiLiang DandanYang JianChen Yi-Han - During embryonic development, the mesoderm undergoes patterning into diverse lineages including axial, paraxial, and lateral plate mesoderm (LPM). Within the LPM, the so-called intermediate mesoderm (IM) forms kidney and urogenital tract progenitor cells, while the remaining LPM forms cardiovascular, hematopoietic, mesothelial, and additional progenitor cells. The signals that regulate these early lineage decisions are incompletely understood. Here, we found that the centrosomal protein 83 (CEP83), a centriolar component necessary for primary cilia formation and mutated in pediatric kidney disease, influences the differentiation of human-induced pluripotent stem cells (hiPSCs) toward IM. We induced inactivating deletions of in hiPSCs and applied a 7-day in vitro protocol of IM kidney progenitor differentiation, based on timed application of WNT and FGF agonists. We characterized induced mesodermal cell populations using single-cell and bulk transcriptomics and tested their ability to form kidney structures in subsequent organoid culture. While hiPSCs with homozygous inactivation were normal regarding morphology and transcriptome, their induced differentiation into IM progenitor cells was perturbed. Mesodermal cells induced after 7 days of monolayer culture of -deficient hiPCS exhibited absent or elongated primary cilia, displayed decreased expression of critical IM genes (, , ), and an aberrant induction of LPM markers (e.g. , , , , ). Upon subsequent organoid culture, wildtype cells differentiated to form kidney tubules and glomerular-like structures, whereas -deficient cells failed to generate kidney cell types, instead upregulating cardiomyocyte, vascular, and more general LPM progenitor markers. Our data suggest that regulates the balance of IM and LPM formation from human pluripotent stem cells, identifying a potential link between centriolar or ciliary function and mesodermal lineage induction. - Source: PubMed
Publication date: 2022/10/12
Mansour FatmaHinze ChristianTelugu Narasimha SwamyKresoja JelenaShaheed Iman BMosimann ChristianDiecke SebastianSchmidt-Ott Kai M - The cardiac conduction system, a network of specialized cells, is required for the functioning of the heart. The basic helix loop helix factors and are required for cardiac morphogenesis and have been implicated in cardiac conduction system development and maintenance. Here we use embryonic and post-natal specific lines to interrogate the role of and in the function of the murine cardiac conduction system. Results demonstrate that loss of HAND1 in the post-natal conduction system does not result in any change in electrocardiogram parameters or within the ventricular conduction system as determined by optical voltage mapping. Deletion of within the post-natal conduction system results in sex-dependent reduction in PR interval duration in these mice, suggesting a novel role for HAND2 in regulating the atrioventricular conduction. Surprisingly, results show that loss of both HAND factors within the post-natal conduction system does not cause any consistent changes in cardiac conduction system function. Deletion of in the embryonic left ventricle results in inconsistent prolongation of PR interval and susceptibility to atrial arrhythmias. Thus, these results suggest a novel role for HAND2 in homeostasis of the murine cardiac conduction system and that HAND1 loss potentially rescues the shortened HAND2 PR phenotype. - Source: PubMed
Publication date: 2022/07/04
George Rajani MGuo ShuaiFirulli Beth ARubart MichaelFirulli Anthony B - Hand1 and Hand2 are transcriptional factors, and knockout mice of these genes show left and right ventricular hypoplasia, respectively. However, their function and expression in human cardiogenesis are not well studied. To delineate their expressions and assess their functions in human cardiomyocytes (CMs) in vitro, we established two triple-reporter human induced pluripotent stem cell lines that express HAND1, HAND2 and either MYH6-driven iRFP670 or tagBFP constitutively and investigated their expression dynamics during cardiac differentiation. On day 5 of the differentiation, HAND1 expression marked cardiac progenitor cells. We profiled the CM subpopulations on day 20 with RNA sequencing and found that mCherry+ CMs showed higher proliferative ability than mCherry- CMs and identified a gene network of LEF1, HAND1, and HAND2 to regulate proliferation in CMs. Finally, we identified CD105 as a surface marker of highly proliferative CMs. - Source: PubMed
Publication date: 2021/07/22
Okubo ChikakoNarita MegumiInagaki AzusaNishikawa MisatoHotta AkitsuYamanaka ShinyaYoshida Yoshinori - Hand genes are required for the development of the vertebrate jaw, heart, peripheral nervous system, limb, gut, placenta, and decidua. Two Hand paralogues, Hand1 and Hand2, are present in most vertebrates, where they mediate different functions yet overlap in expression. In ray-finned fishes, Hand gene expression and function is only known for the zebrafish, which represents the rare condition of having a single Hand gene, hand2. Here we describe the developmental expression of hand1 and hand2 in the cichlid Copadichromis azureus. - Source: PubMed
Publication date: 2021/06/16
Reynolds SamanthaPierce ChristianPowell BenjaminKite AlexandraHall-Ruiz NicholasSchilling ThomasLe Pabic Pierre