Antioxidant _ Hydroxyl Radicals with Special Pholasin® (tube format) _ Volume of test 1 ml
- Known as:
- Antioxidant _ Hydroxyl Radicals Special Pholasin® (tube supplied) _ Volume test 1 milliliter
- Catalog number:
- KSL-ABEL-53
- Product Quantity:
- 100 tests
- Category:
- -
- Supplier:
- Knight Scientific
- Gene target:
- Antioxidant _ Hydroxyl Radicals with Special Pholasin® (tube format) Volume test 1
Related genes to: Antioxidant _ Hydroxyl Radicals with Special Pholasin® (tube format) _ Volume of test 1 ml
- Gene:
- SPOCK1 NIH gene
- Name:
- SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1
- Previous symbol:
- TIC1, SPOCK
- Synonyms:
- testican-1
- Chromosome:
- 5q31.2
- Locus Type:
- gene with protein product
- Date approved:
- 1995-10-02
- Date modifiied:
- 2018-02-23
- Gene:
- SPOCK2 NIH gene
- Name:
- SPARC (osteonectin), cwcv and kazal like domains proteoglycan 2
- Previous symbol:
- -
- Synonyms:
- KIAA0275, testican-2
- Chromosome:
- 10q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2003-01-24
- Date modifiied:
- 2018-02-23
- Gene:
- SPOCK3 NIH gene
- Name:
- SPARC (osteonectin), cwcv and kazal like domains proteoglycan 3
- Previous symbol:
- -
- Synonyms:
- testican-3
- Chromosome:
- 4q32.3
- Locus Type:
- gene with protein product
- Date approved:
- 2003-01-24
- Date modifiied:
- 2018-02-23
- Gene:
- TSC2 NIH gene
- Name:
- TSC complex subunit 2
- Previous symbol:
- TSC4
- Synonyms:
- tuberin, LAM, PPP1R160
- Chromosome:
- 16p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1989-05-25
- Date modifiied:
- 2019-04-23
Related products to: Antioxidant _ Hydroxyl Radicals with Special Pholasin® (tube format) _ Volume of test 1 ml
Related articles to: Antioxidant _ Hydroxyl Radicals with Special Pholasin® (tube format) _ Volume of test 1 ml
- Spock3/Testican-3 is a nervous system-expressed heparan sulfate proteoglycan belonging to a subgroup of the BM-40/SPARC/osteonectin family, the role of which in brain development is unclear. Because Spock1, a member of the Spock family, inhibits their attachment to substrates and the neurite outgrowth of cultured neuronal cells, Spock3 is also thought to be similarly involved in the neuronal development. In the present study, we established a Spock3-mutant mouse harboring a deletion extending from the presumptive upstream regulatory region to exon 4 of the Spock3 locus and performed histological and behavioral studies on these mutant mice. In wild-type (WT) mice, all Spock members were clearly expressed during brain development. In adults, intense Spock1 and Spock2 expressions were observed throughout the entire brain; whereas, Spock3 expression was no longer visible except in the thalamic nuclei. Thus, Spock3 expression is mostly confined to the developmental stage of the brain. In adult mutant mice, the cells of all cortical layers were swollen. The corpus callosum was narrowed around the central region along the rostral-caudal axis and many small spaces were observed without myelin sheaths throughout the entire corpus callosum. In addition, the cortical input and output fibers did not form into thick bundled fibers as well as the WT counterparts did. Moreover, a subpopulation of corticospinal axonal fibers penetrated into the dorsal striatum with moderately altered orientations. Consistent with these modifications of brain structures, the mutant mice exhibited decreased anxiety-like behavior and lowered sociability. Together, these results demonstrate that Spock3 plays an important role in the formation or maintenance of major neuronal structures in the brain. - Source: PubMed
Publication date: 2014/08/19
Yamamoto AyakoUchiyama KojiNara TomokaNishimura NaomichiHayasaka MichikoHanaoka KazunoriYamamoto Tatsuro - Using expression cloning to screen a human fetal kidney cDNA library for regulator(s) of pro-matrix metalloproteinase (MMP)-2 processing mediated by membrane-type (MT) 1 MMP, we isolated a cDNA whose product interfered with pro-MMP-2 activation. It encodes the NH(2)-terminal 313-amino acid region of a calcium-binding proteoglycan, testican 3, with a 3-amino acid substitution at the COOH terminus and thus was named N-Tes. N-Tes comprises a signal peptide, a unique domain, a follistatin-like domain, and a Ca(2+)-binding domain but lacks a COOH-terminal thyroglobulin domain and two putative glycosaminoglycan attachment sites of testican 3. Pro-MMP-2 activation by MT3-MMP was also inhibited by the coexpression of N-Tes. Immunoprecipitation analysis demonstrated direct interaction of N-Tes with either MT1-MMP or MT3-MMP. Expression of testican 1 or testican 3 but not testican 2 also inhibited pro-MMP-2 activation by either MT1-MMP or MT3-MMP. Deletion and substitution of amino acids residues in N-Tes revealed that the unique NH(2)-terminal domain of N-Tes is responsible for the inhibition of pro-MMP-2 activation by MT-MMPs. Expression of N-Tes and testican 3 was detected in normal brain but down-regulated in glioma tissues. Transfection of either the N-Tes or testican 3 gene into U251 glioma cells or Madin-Darby canine kidney cells transformed by erbB2 suppressed their invasive growth in collagen gel. These results suggest that both N-Tes and testican 3 would interfere with tumor invasion by inhibiting MT-MMPs. - Source: PubMed
Nakada MYamada ATakino TMiyamori HTakahashi TYamashita JSato H