Anti-Mouse CD71 (Transferrin Receptor) FITC 100 ug
- Known as:
- Antibody toMouse CD71 (Transferrin Receptor) fluorecein 100 ug
- Catalog number:
- 11-0711-82
- Category:
- -
- Supplier:
- eBioscience
- Gene target:
- Anti-Mouse CD71 (Transferrin Receptor) FITC 100
Related genes to: Anti-Mouse CD71 (Transferrin Receptor) FITC 100 ug
- Gene:
- TFRC NIH gene
- Name:
- transferrin receptor
- Previous symbol:
- -
- Synonyms:
- CD71, TFR1, p90
- Chromosome:
- 3q29
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2014-11-19
Related products to: Anti-Mouse CD71 (Transferrin Receptor) FITC 100 ug
Related articles to: Anti-Mouse CD71 (Transferrin Receptor) FITC 100 ug
- The blood-brain barrier (BBB) is a structure mainly formed by brain capillary endothelial cells (BCEC) whose role is to regulate the exchange of compounds between the blood and the brain. In this process efflux and uptake transporters play a key role. Aim of this study was to compare the two previously established cell lines hCMEC/D3 and hBMEC as BBB cell models for the application of an adenoviral system to transiently express OATP2B1 and Pgp. Comparison of hCMEC/D3 and hBMEC mRNA and protein levels of BBB markers showed a unique expression pattern for each cell line. While showing similar expression of the efflux transporter BCRP, transferrin receptor (TFRC) and of the tight junctions proteins Occludin and ZO-1, hCMEC/D3 displayed higher levels of the endothelial marker PECAM1, VE-cadherin, Von Willebrand Factor (VWF) and of the efflux transporter Pgp. Moreover, measuring integrity of the monolayer by determining the Trans-Endothelial Electrical Resistance (TEER), electrical capacitance (C), and inulin apparent permeability coefficient (P) revealed higher TEER and lower C for hBMEC but comparable P in the two cell lines. Following adenoviral infection, enhanced OATP2B1 and Pgp expression and functionality could be observed only in hBMEC. Importantly, the adenoviral expression system did not affect expression of BBB markers and permeability in both cell lines. Taken together, our results provide first evidence that hBMEC is an applicable human BBB cell model in which adenoviral infection can be used to transiently express and investigate transporters of interest. - Source: PubMed
Publication date: 2024/05/12
Taggi ValerioSchäfer Anima MDolce AsaélMeyer Zu Schwabedissen Henriette E - COVID-19 is characterized by a wide range of clinical manifestations, where aging, underlying diseases, and genetic background are related to worse outcomes. In the present study, the differential expression of seven genes related to immunity, , , , , , , and , was analyzed in individuals with COVID-19 diagnoses of different disease severities. Two-step RT-qPCR was performed to determine the relative gene expression in whole-blood samples from 160 individuals. The expression of ( < 0.05) and ( < 0.05) was higher in moderate hospitalized cases than in severe ones. Increased gene expression of (OR = 0.64, CI = 0.52-0.79; = 0.001), (OR = 0.581, CI = 0.43-0.79; = 0.001), and (OR = 0.544, CI = 0.39-0.69; < 0.001) was associated with a lower risk of requiring IMV. Moreover, (OR = 0.646, CI = 0.50-0.83; = 0.001), (OR = 0.57, CI = 0.39-0.83; = 0.003), (OR = 0.80, CI = 0.653-0.979; = 0.03), and (OR = 0.827, CI = 0.69-0.991; = 0.039) expression was associated with patient survival. In conclusion, the relevance of , , and in controlling the viral infection was confirmed. - Source: PubMed
Publication date: 2024/04/24
Gajate-Arenas MalenaFricke-Galindo IngridGarcía-Pérez OmarDomínguez-de-Barros AngélicaPérez-Rubio GloriaDorta-Guerra RobertoBuendía-Roldán IvetteChávez-Galán LeslieLorenzo-Morales JacobFalfán-Valencia RamcésCórdoba-Lanús Elizabeth - Cadmium (Cd) and excess molybdenum (Mo) are multiorgan toxic, but the detrimental impacts of Cd and/or Mo on poultry have not been fully clarified. Thence, a 16-week sub-chronic toxic experiment was executed with ducks to assess the toxicity of Cd and/or Mo. Our data substantiated that Cd and Mo coexposure evidently reduced GSH-Px, GSH, T-SOD, and CAT activities and elevated HO and MDA concentrations in myocardium. What is more, the study suggested that Cd and Mo united exposure synergistically elevated Fe content in myocardium and activated AMPK/mTOR axis, then induced ferroptosis by obviously upregulating ACSL4, PTGS2, and TFRC expression levels and downregulating SLC7A11, GPX4, FPN1, FTL1, and FTH1 expression levels. Additionally, Cd and Mo coexposure further caused excessive ferritinophagy by observably increasing autophagosomes, the colocalization of endogenous FTH1 and LC3, ATG5, ATG7, LC3II/LC3I, NCOA4, and FTH1 expression levels. In brief, this study for the first time substantiated that Cd and Mo united exposure synergistically induced ferroptosis and excess ferritinophagy by AMPK/mTOR axis, finally augmenting myocardium injure in ducks, which will offer an additional view on united toxicity between two heavy metals on poultry. - Source: PubMed
Publication date: 2024/05/08
Huang BingyanNie GaohuiDai XueyanCui TingPu WenjingZhang Caiying - The aim of this study was to evaluate the effects of two forms of tobacco smoking, cigarettes and water pipe smoking (WPS), on the expression of a panel of salivary proteins in healthy adults. Three groups of age and gender-matched participants were enrolled in the study: never-smokers, cigarette smokers and WPS (N = 55 per group). Expression of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), endothelin and transferrin in unstimulated whole saliva was estimated using enzyme-linked immunosorbent assays. Statistical analysis consisted of one-way ANOVA and Tukey's post hoc tests, in addition to bioinformatics analysis. VEGF expression was the least in WPS (51.1 ± 14.5 pg/ml) compared to both controls (150.1 ± 13.8 pg/ml) and cigarette smokers (93 ± 9.9 pg/ml), with a significant difference in WPS (p < 0.001) and cigarette smokers (p < 0.01) compared to controls. Furthermore, transferrin showed the weakest expression in the WPS group (1238 ± 261.4 pg/ml) compared to controls (2205.6 ± 298.6 pg/ml) (p = 0.05) and cigarette smokers (1805.4 ± 244 pg/ml). Neither EGF nor endothelin expression showed any statistical difference between the groups (p > 0.05). Gene-gene interaction network demonstrated that FLT1, TFRC, KDR, VEGFB and PGF genes had the highest potential for interaction with the studied proteins. Further functional annotations on the identified markers in the interaction network were performed to identify HIF-1 pathways among the most relevant pathways. In conclusion, smoking habits alter the expression of salivary VEGF and transferrin, which may correspond to early sub-clinical changes in the oral mucosa. The clinical relevance of these salivary changes requires further research. - Source: PubMed
Publication date: 2024/05/06
Alrashdan Mohammad SAl-Shorman HishamBouzid AmalAl-Dwairi AhmedAlazzam MelanieAlqudah Mohammad - With the aim to show the feasibility of early tumor shrinkage (ETS) concept implementation into daily clinical practice in the Czech Republic, a non-interventional, multicentric, single arm, prospective study in real world set-up was performed. - Source: PubMed
Fínek JVočka MBauer JKubala EZycháčková KŠedivá MGharibyar MKocáková IČmuchařová EVaňková M