Polyclonal Rabbit ASAH3L Antibody
- Known as:
- Polyclonal Rabbit ASAH3L Antibody
- Catalog number:
- KA0308
- Product Quantity:
- 100ul
- Category:
- -
- Supplier:
- KareBay
- Gene target:
- Polyclonal Rabbit ASAH3L Antibody
Related genes to: Polyclonal Rabbit ASAH3L Antibody
- Gene:
- ACER2 NIH gene
- Name:
- alkaline ceramidase 2
- Previous symbol:
- ASAH3L
- Synonyms:
- FLJ41587, ALKCDase2
- Chromosome:
- 9p22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2004-05-28
- Date modifiied:
- 2016-10-17
Related products to: Polyclonal Rabbit ASAH3L Antibody
Related articles to: Polyclonal Rabbit ASAH3L Antibody
- To investigate the effects of chrysotile asbestos exposure on ribosomal DNA (rDNA) copy number variation and DNA damage response (DDR) in human pleural mesothelial cells (MeT-5A) . In April 2024 MeT-5A cells were exposed to chrysotile asbestos at doses of 2.5 μg/cm(2), 5 μg/cm(2) once a week for four weeks, and the cells were collected at weeks 1 and 4 (P1 and P7). The 45S rDNA copy number, 5S rDNA copy number, ACER2, PA2G4, and ZNF385A mRNA expression were determined by quantitative real-time PCR (qPCR) assay. Cell cycle, apoptosis, and DNA damage in the cells were detected using a Muse cell analyzer. The data were statistically anayzed using SPSS 24.0 software. Measurement data conforming to normal distribution were expressed as mean±standard deviation. One-way analysis of variance was used for comparisons among multiple groups, and the least significant difference () tset was used for further pairwise comparisons. Compared with the control group, the 45S and 5S rDNA copy number in the 5 μg/cm(2) exposed group increased at both P1 and P7 generations (<0.05). Compared with the control group, the mRNA expression level of the nucleolar protein ACER2 decreased in the chrysotile asbestos exposed groups at both P1 and P7 generations (<0.05), while the mRNA expression level of ZNF385A increased in the 5μg/cm2 exposed group (<0.05). The mRNA expression level of PA2G4 increased in the chrysotile asbestos exposure group at P7 (<0.05) but decreased in the exposure group at P1 (<0.05). Compared with the control group, the early, late and total apoptosis rates in the chrysotile asbestos exposure groups showed statistically significant differences at different time points (<0.05). Compared with the control group, the proportion of cells in the G0/G1 phase decreased in the chrysotile asbestos exposure group at P7 (<0.001), while the proportion of cells in the S phase increased (<0.05), and the proportion of G2/M phase increased in the 5 μg/cm2 exposure group (<0.05), with statistically significant differences. Compared with the control group, the DNA double-strand break rate in the exposure groups at P1 and P7 and the H2A.X phosphorylation rate in the exposed group at P7 increased, with statistically significant differences (<0.05) . Chrysotile asbestos exposure caused changes in rDNA copy number and mRNA expression levels of related nucleolin genes in Met-5A cells, inducing apoptosis, cell cycle arrest, and DNA damage. - Source: PubMed
Su XLi Y XLiu J QHuang JJiang Z QLou J L - This study investigated the metabolic and pathological effects of a high-fat diet (HFD) in db/db mice and evaluated the therapeutic efficacy of various Coenzyme Q10 (CoQ10) products. We aimed to determine whether HFD-induced mitochondrial damage can be improved by different CoQ10 products through either repairing mitochondrial injury or increasing mitochondrial bioenergy, thereby addressing the root cause of oxidative stress. - Source: PubMed
Publication date: 2025/12/30
Kuo Chen-LingWu Chih-ChungCheng Yu-ShanHuang Ching-ShanLiu Chin-SanSu Shih-Li - Meat quality is a complex trait affected by genetic and environmental factors. Intramuscular fat (IMF) is one of the most important factors affecting meat quality. However, there is little research focusing on IMF deposition in rabbits. In this study, we compared the meat quality and nutritional composition of New Zealand White rabbits (NZWRs) and Yufeng Yellow rabbits (YFYRs). The results showed that the drip loss of longissimus dorsi muscle (LD muscle) of YFYR was significantly lower than that of NZWR (p < 0.05), and the pH of YFYR was significantly higher than that of NZWR (p < 0.001). Compared with NZWR, the content of IMF, C22:6 n3, C16:0, C20:3 n3, and n-3 PUFA in the LD muscle of YFYR was significantly increased (p < 0.05), while the content of C15:0 was significantly decreased (p < 0.05). RNA-Seq and miRNA-Seq of the LD muscle tissue were performed to measure the mRNA and miRNA expressions of both NZWR and YFYR. A total of 1175 differentially expressed genes (DEGs) were identified between the two rabbit species, including 340 upregulated and 835 downregulated mRNAs in NZWR. A total of 138 differentially expressed miRNAs (DE-miRNAs) were identified, including 31 upregulated and 107 downregulated miRNAs in NZWR. Functional enrichment analysis revealed that the target genes of differential genes and miRNAs were enriched in lipid binding, long-chain fatty acid binding, lipid metabolic process, and fatty acid metabolism, as well as the PPAR signaling pathway, glycerol phospholipid metabolism, and other pathways related to lipid deposition. MiR-973-y, ocu-miR-124-3p, ocu-miR-2355-5p, miR-197-y, and ocu-miR-301b-3p are key miRNAs regulating IMF deposition, and GPX7, GPD2, BDH1, PCYT1B, and ACER2 are key target genes related to lipid deposition and metabolism. In addition, correlation analysis suggested that the IMF content was positively correlated with PAFAH2 and HSD11B2 genes (p < 0.05), while negatively correlated with PRKAG2 (p < 0.01). Taken together, this study suggests that YFYR has relatively better meat quality and nutritional value relative to NZWR; the identified DEGs and target miRNAs provide strong evidence for further studying the regulatory mechanism of IMF deposition in rabbits. - Source: PubMed
Ou ShuodiJiang YixuanHan WenxiangNiu HuidongJia TaiqingLi BoCai HanfangWang YapingChen ZhiLi MingXu Huifen - Abdominal fat deposition is an important economic trait in poultry, as excessive accumulation reduces feed efficiency and carcass yield. The gut microbiota is known to influence host energy metabolism and fat storage, suggesting its potential involvement in fat deposition. This study examined the relationship between intestinal microbiota and abdominal fat deposition in an F population derived from Cherry Valley Ducks (♂) × Runzhou Crested White Ducks (♀) at 42 days of age. Based on abdominal fat rate, ducks with values of 0-0.75% and 1.5-2.25% were defined as the low (LF) and high (HF) abdominal fat groups, respectively. A combined multi-omics approach was used, including 16S rRNA gene sequencing, metagenomics, and whole transcriptomics, to compare high and low abdominal fat rate groups. 16S rRNA gene sequencing results showed that the cecum had the highest microbial diversity among all intestinal segments (duodenum, jejunum, ileum, and rectum) and was significantly enriched in carbohydrate metabolism pathways, highlighting its key role in nutrient utilization and growth. Therefore, the cecum was selected for further analysis. Metagenomic analysis of the cecum contents revealed significantly different intestinal microbial β diversity between the high and low abdominal fat rate groups ( < 0.05). The low abdominal fat rate group was enriched in beneficial microorganisms such as , , , Ruminococcaceae, Veillonellaceae (Clostridiales), and Firmicutes. Conversely, the high abdominal fat rate group was characterized by an increased abundance of Bacteroidetes, including both beneficial and potentially pathogenic taxa such as and Eggerthellales. The integrated analysis of metagenomic and whole transcriptome sequencing showed that Firmicutes and Bacteroidetes were not only related to energy metabolism, lipid metabolism, and amino acid metabolism, but also to the expression of , , , , , , and . In addition, Firmicutes and Bacteroidetes were also associated with 7 lncRNAs: XR_003493494.1, XR_003492471.1, XR_001190174.3, TCONS_00005095, XR_001190238.3, TCONS_00005095, and XR_003492841.1. In conclusion, this study highlights that the cecal microbiota is closely associated with abdominal fat deposition in ducks, elucidating its potential influence on host metabolism and gene expression. These findings enhance our understanding of the gut microbiota's relationship with obesity and offer new strategies to modulate gut-microbe interactions to reduce abdominal fat accumulation in poultry. - Source: PubMed
Publication date: 2025/11/24
Wang ZhixiuYang ChunyanLi YanDong BingqiangSong QianqianBai HaoJiang YongChang GuobinChen Guohong - Among the DNA-damaging agents commonly used in clinical settings doxorubicin has emerged as one of the most effective treatments for Triple-negative breast cancer (TNBC). Our limited understanding about the molecular mechanisms underlying the short- and long-term responses of TNBC cells to DNA damage induced by drugs like doxorubicin is a hurdle to improve the efficacy of the treatment or overcome the drug resistance. In this study, we aimed to elucidate the immediate response of the TNBC cells to doxorubicin and compare these responses with those of doxorubicin-resistant cells through transcriptome analysis. Transcriptome analysis revealed that doxorubicin significantly upregulates the expression of TP53 target genes, including CDKN1A, TIGAR, TP53INP1, PPM1D, and ACER2. Notably, doxorubicin-resistant TNBC cells failed to increase the expression of these genes, except for CDKN1A, upon doxorubicin treatment. Moreover, treatment with etoposide as another DNA-damaging drug increased the expression of CDKN1A, TP53INP1, and ACER2 in a TP53-independent manner. Collectively, this study highlights the critical role of TP53 target genes in the immediate response of TNBC cells to DNA-damaging agents like doxorubicin and etoposide. It also reveals distinct molecular mechanisms regulating their expression in resistant versus sensitive cells, offering potential therapeutic targets to improve treatment strategies for TNBC. - Source: PubMed
Publication date: 2025/12/04
Shekari AysanPazhang YaghubMaadi Hamid