Polyclonal Rabbit DDX55 Antibody
- Known as:
- Polyclonal Rabbit DDX55 Antibody
- Catalog number:
- KA1040
- Product Quantity:
- 100ul
- Category:
- -
- Supplier:
- KareBay
- Gene target:
- Polyclonal Rabbit DDX55 Antibody
Related genes to: Polyclonal Rabbit DDX55 Antibody
- Gene:
- DDX55 NIH gene
- Name:
- DEAD-box helicase 55
- Previous symbol:
- -
- Synonyms:
- KIAA1595
- Chromosome:
- 12q24.31
- Locus Type:
- gene with protein product
- Date approved:
- 2003-06-13
- Date modifiied:
- 2016-01-07
Related products to: Polyclonal Rabbit DDX55 Antibody
Related articles to: Polyclonal Rabbit DDX55 Antibody
- Inorganic polyphosphate (polyP), a conserved phosphate polymer, modulates protein function across diverse biological systems, yet its human protein interactome remains poorly defined. While prior studies identified denaturation-resistant polyP modifications on lysine-rich sequences in yeast and bacteria, such targets in human proteins were previously unknown. Here, we performed a systematic screen of 57 lysine-rich human proteins, selected via mass spectrometry and bioinformatics, identifying 41 proteins that exhibit denaturation-resistant polyP modifications. This marks the first identification of lysine-rich motifs as polyP targets in human proteins. Through mutagenesis and binding assays, we establish these lysine-rich motifs, rather than traditional PASK domains, are critical for polyP binding, with consecutive lysine residues and intrinsic disorder as key determinants. Functional assays demonstrate that polyP binding inhibits phase separation of the transcriptional regulator NKAP, suppresses K-RAS GTPase activation, and reduces DDX55 helicase activity, revealing direct regulatory mechanisms. This work not only marks the first identification of denaturation-resistant polyP modifications in human proteins but also provides a comprehensive resource for investigating polyP's roles in human cellular homeostasis and disease. - Source: PubMed
Publication date: 2025/11/29
Yang ZhiyunJin JinLehotsky KirstenMartins IsabellaBelrose EthanNeville NolanJia Zongchao - The 3' untranslated regions (3'UTRs) of mRNAs play a critical role in controlling gene expression and function because they contain binding sites for microRNAs and RNA binding proteins (RBPs) that alter mRNA stability, localization, and translation. Most mRNA 3' ends contain multiple polyadenylation sites (PAS) that can be utilized in condition-specific manners, a process known as alternative polyadenylation (APA). However, the mechanisms driving the regulation of APA remain poorly characterized. - Source: PubMed
Publication date: 2025/11/12
Gazzara Matthew RCater TimothyMallory Michael JBarash YosephLynch Kristen W - Naïve T cells are maintained in a homeostatic state to preserve a stable T cell pool with diverse T cell receptor (TCR) repertoires, ensuring preparedness for priming. However, the underlying mechanisms controlling naïve T cell homeostasis and priming remain unclear. Leveraging a machine learning-based functional genetic screen, we identified () as the top factor responsible for naïve T cell homeostasis. DDX55 was highly expressed in naïve T cells and suppressed enhancer- and promoter-like transposable elements (TEs) near T cell activation-associated genes. loss led to derepression of these TEs, resulting in TE-derived R loops and genomic instability, ultimately disrupting naïve T cell homeostasis and abolishing T cell proliferation. Mechanistically, DDX55-targeted TEs harbored myelocytomatosis oncogene (MYC)-binding motifs. DDX55 directly bound MYC and restricted its access to these TE loci, thereby preventing inappropriate TE activation in naïve T cells. Thus, naïve T cells exploit DDX55 as a vital regulator of T cell activation, ensuring their genomic stability and homeostatic maintenance. - Source: PubMed
Publication date: 2025/09/05
Wu MengyueLinghu KepanYin QiminHe PingYu XinyangHu PengHe RongyingDu QinyunWang ShengliChen XiWang ShaohuiWu XingyunZhang MingfuPeng KerenWang XiangLiu JuxiuDeng DongCao GuangchaoWang KuiMeng XianliYang QuanliMu DezhiYin ZhinanChen LuTong Jiyu - The 3' untranslated regions (3'UTRs) of mRNAs play a critical role in controlling gene expression and function because they contain binding sites for microRNAs and RNA binding proteins (RBPs) that alter mRNA stability, localization, and translation. Most mRNA 3' ends contain multiple polyadenylation sites (PAS) that can be utilized in condition-specific manners, a process known as alternative polyadenylation (APA), however the mechanisms driving the regulation of APA remain poorly characterized. By integrating a large set of over 500 RNA binding protein (RBP) depletion and binding experiments across two cell lines generated by the ENCODE consortium, we uncovered a number of RBPs in each cell type whose depletion leads to widespread alteration of 3' UTR patterns. These include not only known regulators of APA, but also many putative novel regulators of 3'UTR isoform expression. We focused analysis on the largely unstudied DEAD box RNA helicase, DDX55, and validate its novel role in 3'UTR isoform regulation using molecular assays and targeted 3' end sequencing experiments. Our findings identify DDX55 as a new regulator of APA, particularly at PAS that contain features of RNA secondary structure. Our data also suggest additional previously unrecognized regulators of 3' UTR processing and differential stability. - Source: PubMed
Publication date: 2025/05/08
Gazzara Matthew RCater TimothyMallory Michael JBarash YosephLynch Kristen W - Although numerous molecular classifications are available to predict the prognosis of patients with hepatocellular carcinoma (HCC), they are still unsatisfactory. Forkhead box O3 (FOXO3) has been widely reported as a transcription factor involved in human cancers, but its role in HCC remains controversial. The present study aimed to explore the role of FOXO3 in HCC, as well as to identify biomarkers and construct prognostic models based on FOXO3. FOXO3 was highly expressed in HCC and was closely associated with poor prognosis in The Cancer Genome Atlas (the training set) and International Cancer Genome Consortium (the validation set). Subsequently, a co-expression network indicated that the red modules were closely related to FOXO3. Five key FOXO3-related genes [DEAD-box helicase 55 (DDX55), RAB10, member RAS oncogene family (RAB10), RAB7A, TATA-box binding protein associated factor, RNA polymerase I subunit B (TAF1B) and TAF3] were obtained using Cox-least absolute shrinkage and selection operator analyses. The 5-gene signature successfully predicted the prognosis of patients with HCC in both the training and validation sets. Enrichment analysis suggested marked differences in AKT and cell cycle-related (E2F targets and G/M checkpoints) pathways between HCC subgroups. Furthermore, the tumor microenvironment analysis suggested that the difference in the distribution of M2 macrophages among various subgroups may contribute to the poor prognosis using the CIBERSORTx framework. Furthermore, the mRNA and protein expressions of DDX55, RAB10, RAB7A, TAF1B and TAF3 were found to be higher in HCC tissues compared with paracancerous tissues using RT-qPCR and western blotting. Additionally, knockdown of RAB10, RAB7A and TAF3 inhibited proliferation of Huh7 cells, assessed by a Cell Counting Kit-8 assay. In conclusion, a novel FOXO3-related model was constructed and revealed that RAB10, RAB7A and TAF3 may be potential molecular targets or biomarkers for HCC. - Source: PubMed
Publication date: 2025/03/13
Guan SongmeiLin QiangHuang PeiwuLin KangqiangDuan Shigang