Polyclonal Rabbit CYSLTR1 Antibody
- Known as:
- Polyclonal Rabbit CYSLTR1 Antibody
- Catalog number:
- KA0963
- Product Quantity:
- 100ul
- Category:
- -
- Supplier:
- KareBay
- Gene target:
- Polyclonal Rabbit CYSLTR1 Antibody
Related genes to: Polyclonal Rabbit CYSLTR1 Antibody
- Gene:
- CYSLTR1 NIH gene
- Name:
- cysteinyl leukotriene receptor 1
- Previous symbol:
- -
- Synonyms:
- CysLT1, CysLT(1), CYSLT1R
- Chromosome:
- Xq21.1
- Locus Type:
- gene with protein product
- Date approved:
- 2002-08-29
- Date modifiied:
- 2016-10-05
Related products to: Polyclonal Rabbit CYSLTR1 Antibody
Related articles to: Polyclonal Rabbit CYSLTR1 Antibody
- Diabetic kidney disease (DKD) is a leading cause of renal failure. Inflammation of the renal tubules and interstitium is a critical factor in the progression of DKD; however, the key regulatory genes and characteristics of the immune microenvironment remain poorly understood. This study aims to identify key inflammatory biomarkers in the renal tubule tissues of DKD patients and to elucidate their potential immunoregulatory mechanisms. By integrating multiple GEO transcriptome datasets and employing differential expression analysis, weighted gene co-expression network analysis (WGCNA), and machine learning algorithms (LASSO, Random Forest), we identified arachidonate 5-lipoxygenase (ALOX5) as a crucial feature gene of renal tubular inflammation in DKD. Clinical correlation analysis revealed that ALOX5 is significantly upregulated in DKD tissues, with high expression closely associated with decreased glomerular filtration rate and infiltration of M1 macrophages. Additionally, combining single-cell sequencing pseudotime analysis and multiplex immunohistochemistry (mIHC), we demonstrated that ALOX5 and its partner protein ALOX5AP are primarily expressed in CD68[Formula: see text] macrophages infiltrating the renal interstitium. They exhibit a high degree of co-localization with NF-κB/p65, iNOS, and CYSLTR1, suggesting that they may mediate the pro-inflammatory polarization of macrophages through the leukotriene-NF-κB axis. Finally, based on molecular docking and ADMET analysis, we screened the natural small molecule honokiol as a potential inhibitor of ALOX5, which possesses favorable pharmacokinetic properties. This study suggests that ALOX5 is a potential biomarker of immune microenvironment imbalance in DKD and provides a rationale for further investigation of targeted anti-inflammatory strategies, with honokiol as a candidate compound. - Source: PubMed
Publication date: 2026/03/19
Lu WeiDeng YiyaoZhai LikangZhang YongqiangYang DieYang KunmingLu XueZhang JuXue QiulingLuo LunjuLiu MingmingRen HongyanXu XinAo DengmeiLiu LuYu FangfangMa YuanZha YanYuan Jing - Meat quality traits are typically regulated by multiple genes, each contributing a small effect. In this study, to pinpoint candidate genes involved in meat quality traits, we performed transcriptome profiles of porcine (LD) muscle and applied machine learning (ML) models to analyze RNA-seq data. We also carried out Gene Set Enrichment Analysis (ssGSEA), Weighted gene co-expression network analysis (WGCNA) and functional validation of putative target genes to better support the biological relevance of our findings. - Source: PubMed
Publication date: 2026/03/14
Ma ShuyaHu XiezongYang JianweiShi KunpengZhang XiaodongDing YueyunWu XudongZhu MengtingYin ZongjunZheng Xianrui - The aim of this study was to systematically explore the potential molecular mechanisms by which aflatoxin B1 (AFB1) may trigger asthma using network toxicology and molecular docking. Potential targets related to asthma caused by AFB1 were obtained from databases, such as PubChem, ProTox, ADMETab, and GeneCards. The targets most significantly related to asthma were further screened using STRING and Cytoscape and analyzed using GO and KEGG enrichment. Finally, molecular docking and visualization were performed using AutodockVina 1.2.2 and PyMOL 2.5 to further determine the affinity between AFB1 and the core targets. We identified 31 potential targets associated with AFB1 exposure and asthma, including PTGS2, ADRB2, CysLTR1, PTGS1, and others. The enrichment analysis revealed that the core targets of AFB1-induced asthma were neuroactive ligand‒receptor interactions, the calcium signaling pathway, and the adipocyte catabolism-related signaling pathway. Molecular docking results revealed that AFB1 exhibited good affinity for the core targets. In the present study, the potential mechanisms involved in AFB1-induced asthma were elucidated, new insights into how environmental toxins trigger asthma were provided, and a theoretical foundation for asthma prevention and treatment was established. - Source: PubMed
Publication date: 2026/01/20
Yu ZhiyueGao MingWu XiaowenLi JiaxiangLiu BaijunWang RuiTan JingYu XiangyanGeng Limei - The main goal of this study is the identification of existing drugs that could be repurposed as antagonists of the V2R, a GPCR controlling renal water balance and involved in abnormal cell proliferation, cancer, and renal cyst enlargement. Given its clinical importance, we carried out the reverse screening of a collection of 1882 existing drugs to repurpose them towards V2R by employing PLATO, a home-built target fishing AI-based platform. Five drugs were shortlisted as promising candidates for V2R: cabergoline, clopidogrel, cloxacillin, perphenazine, and zafirlukast. Renal collecting duct MCD4 cells, stably expressing human V2R and AQP2, were used for experimentally testing the effects of the prioritized drugs on V2R responses. FRET studies were conducted to assess whether these drugs affect the DDAVP-induced cAMP responses. Interestingly, zafirlukast, at single-digit nanomolar concentration significantly reduced the DDAVP-dependent cAMP production and water reabsorption, with effects comparable to tolvaptan, a well-known selective V2R antagonist. The molecular rationale behind the observed binding was explained by mapping on the V2R a molecular cleft superimposable to CysLTR1 binding site of zafirlukast. Induced-fit docking simulations demonstrated that zafirlukast engages V2R by adopting a binding conformation closely resembling that of X-ray solved vasopressin. Taken together, our results support the repurposing of zafirlukast as a promising V2R antagonist candidate. - Source: PubMed
Publication date: 2025/12/25
Trisciuzzi DanielaAngelini InesGambacorta NicolaCentrone MariangelaCiriaco FulvioSiragusa LydiaDi Mise AnnaritaRanieri MariannaValenti GiovannaGuaragnella NicolettaAltomare Cosimo DamianoCotecchia SusannaTamma GraziaNicolotti Orazio - The cysteinyl leukotriene receptor 1 (CysLTR1) is known as a potent lipid mediator with a well-established role in inflammatory regulation and lung disease. While its involvement in immune cell recruitment has been previously reported, its broader impact on pulmonary metabolism remains poorly understood. - Source: PubMed
Publication date: 2025/11/24
Adeosun Wilson BamisePoswayo Sibongiseni KlParihar Suraj PLoots Du Toit