SERPINF1 PEDF IgG1 antibody Ab host: Mouse
- Known as:
- SERPINF1 PEDF IgG1 (anti-) Antibody production species: Mouse
- Catalog number:
- 'SM6032S
- Product Quantity:
- 50 Вµl
- Category:
- -
- Supplier:
- ACR
- Gene target:
- SERPINF1 PEDF IgG1 antibody host: Mouse
Related genes to: SERPINF1 PEDF IgG1 antibody Ab host: Mouse
- Gene:
- PPP1R18 NIH gene
- Name:
- protein phosphatase 1 regulatory subunit 18
- Previous symbol:
- KIAA1949
- Synonyms:
- phostensin
- Chromosome:
- 6p21.33
- Locus Type:
- gene with protein product
- Date approved:
- 2004-03-02
- Date modifiied:
- 2016-10-05
- Gene:
- SERPINF1 NIH gene
- Name:
- serpin family F member 1
- Previous symbol:
- PEDF
- Synonyms:
- EPC-1, PIG35
- Chromosome:
- 17p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1993-05-18
- Date modifiied:
- 2018-03-02
Related products to: SERPINF1 PEDF IgG1 antibody Ab host: Mouse
Related articles to: SERPINF1 PEDF IgG1 antibody Ab host: Mouse
- Neonatal calf health critically depends on successful passive transfer of immunity (PTI) via colostrum. Although PTI is routinely assessed by serum IgG concentration, associated systemic proteomic responses remain poorly defined. This study aimed to relate distinct PTI categories to neonatal calf serum proteomes and to evaluate colostrum proteomes to identify proteins influencing passive transfer success. Forty Holstein cow-calf pairs were enrolled. Calf serum was collected before colostrum feeding (0 h) and at 48 h. Serum IgG was quantified by ELISA, and calves were classified at 48 h as failed (FPTI, <1000 mg/dL, n = 11), adequate (APTI, 1000-2000 mg/dL, n = 11), or excellent (EPTI, >2000 mg/dL, n = 18). For proteomic analyses, five calves were purposefully selected in each group; serum samples were pooled by PTI group and time point, and colostrum samples by PTI group, then analyzed by LC-MS/MS in DIA mode. Proteomics identified 54 proteins in colostrum and 80 in serum, 27 of which were shared between matrices. Post-colostrum intake, 21 (FPTI), 5 (APTI), and 30 (EPTI) serum proteins changed significantly from 0 to 48 h. In EPTI calves, haptoglobin (Hp) (51.10-fold) and pigment epithelium-derived factor (PEDF) (27.24-fold) showed the strongest upregulation. At 48 h, lipopolysaccharide-binding protein (LBP) and haemoglobin subunit epsilon (Hb-ε) were lower in EPTI, whereas PEDF, polymeric immunoglobulin receptor (PIGR), and SERPINA3-6 were higher, consistent with coordinated activation of acute-phase, mucosal/transport, and protease-antiprotease pathways. These findings indicate that PTI involves coordinated proteomic changes beyond IgG alone and highlight candidate biomarkers of successful passive immunity in neonatal calves. - Source: PubMed
Publication date: 2026/07/17
Kaçar YiğitTopal OnurMecitoğlu ZaferSağlam NezaketFthenakis George CBatmaz Hasan - At present, colorectal cancer (CRC) ranks as the third most prevalent cancer globally and is the second most common cause of mortality associated with cancer. The expression of A Disintegrin and Metalloproteinase with Thrombospondin Motifs 5 (ADAMTS5) is upregulated in CRC, and high ADAMTS5 expression strongly correlates with an unfavorable prognosis. However, the function of ADAMTS5 in CRC remains unknown. This study aimed to investigate the role of ADAMTS5 in CRC and its potential mechanisms of action. The results showed that ADAMTS5 expression was higher in CRC tissues than in paracancerous tissues. Bioinformatics analysis revealed that its expression gradually increased with tumor progression and that high expression was correlated with poor prognosis. ADAMTS5 knockdown suppressed the proliferation and migration of HCT116 and HT29 cells, and ADAMTS5 inhibition suppressed epithelial-mesenchymal transition (EMT) in HCT116, HT29, and patient-derived organoids. Mechanistic analysis using the STRING database revealed that ADAMTS5 expression was strongly correlated with the Wnt signaling pathway. Western blotting analysis confirmed that ADAMTS5 inhibition suppressed tumor cell invasion and migration and blocked EMT in vitro through the PEDF/Wnt/β-catenin pathway. Furthermore, ADAMTS5 inhibition significantly inhibited tumor proliferation and metastasis within a nude mouse CRC liver metastasis model. These findings indicated that inhibition of ADAMTS5 could suppress CRC progression and metastasis via the PEDF/Wnt/β-catenin pathway. - Source: PubMed
Sun XuanZhang HanqingHu YanJiao ShengyuYao ZeshengYi KeGu GuojianXu Xiaohui - Dry eye disease (DED) is a multifactorial disorder characterized by tear film instability, ocular surface damage, and inflammation. This study investigates the molecular landscape and the therapeutic potential of umbilical cord mesenchymal stem cell-derived extracellular vesicles (UCMSC-EVs) as a standardized, cell-free treatment for DED. UCMSC-EVs were produced from UCMSCs in a scalable 3D bioreactor system and enriched via tangential flow filtration (TFF). The physicochemical properties of UCMSC-EVs were characterized. The molecular cargo of UCMSC-EVs was analyzed via proteomics analysis and microRNA (miRNA) microarray profiling across multiple production lots. Functional potency of UCMSC-EVs was assessed using a human corneal epithelial cell line (HCE-S) wound healing assay and two human inflammation models. UCMSC-EVs exhibited a consistent cargo profile enriched with proteins and miRNAs targeting DED-related pathways, with high proteomic correlation (Pearson's > 0.8) across independent lots. Functional assays demonstrated that UCMSC-EVs promoted HCE-S wound healing and suppressed pro-inflammatory cytokines in a dose-dependent manner. Furthermore, a significant negative correlation was identified between PEDF (Pigment Epithelium-Derived Factor) cargo concentration and interferon gamma (IFN-γ) secretion (Pearson's = -0.724, = 0.005), providing a quantitative link between molecular identity and anti-inflammatory potency. UCMSC-EVs possess favorable physicochemical and molecular characteristics, and demonstrate robust regenerative and anti-inflammatory properties . The high manufacturing consistency and the identification of key molecular drivers, such as PEDF, support the potential of UCMSC-EV as a well-characterized and viable cell-free therapy for mitigating DED progression. - Source: PubMed
Publication date: 2026/06/16
Hsu Qiao-YuHsiung Chia-NiCheng Hsin-HungLien Wen-YuLin Wen-HsienSieber MartinKang Chi-Chih - Duchenne muscular dystrophy (DMD) is a progressive neuromuscular disorder for which monitoring biomarkers are urgently needed. We aimed to evaluate whether proteins in serum can accurately monitor patients' function within the duration of a clinical trial. - Source: PubMed
Publication date: 2026/07/13
Degan ChiaraTobin Rebecca Ade Vries Sharon IJiménez-Requena AlbertPeco AmelaGuglieri MichelaDiaz-Manera Jordivan der Burgt Yuri E Mvan Vlijmen Bart J M Hathout YetribSzigyarto Cristina Al-KhaliliDang Utkarsh JTsonaka RoulaSpitali Pietro - Photoreceptors are highly specialized neurons that depend on continuous membrane renewal and tightly regulated lipid homeostasis to maintain their visual function. Age-associated disruptions of these processes increase cellular stress and contribute to photoreceptor degeneration. Pigment epithelium-derived factor (PEDF) is a potent neuroprotective factor in the retina, and the identification of its receptor, PEDF-R (PNPLA2), has provided key mechanistic insight into how PEDF signaling is coupled to lipid metabolism. The present review examines the molecular and cellular mechanisms underlying PEDF-R function in photoreceptors and the retinal pigment epithelium (RPE). PEDF-R acts as a multifunctional enzyme with phospholipase and lipase activities that link extracellular PEDF binding to intracellular lipid remodeling. Through these activities, PEDF-R has been associated with processes essential for photoreceptor survival such as membrane phospholipid turnover, mitochondrial integrity, calcium homeostasis, and redox balance. In addition, PEDF-R contributes to retinoid metabolism and lipid processing associated with outer-segment renewal in the RPE. We further discuss how disruption of the PEDF-PEDF-R pathway impairs lipid homeostasis, promotes oxidative and inflammatory stress, and increases susceptibility to age-related retinal degeneration. These insights position PEDF-R as a key contributor to photoreceptor homeostasis and a potential therapeutic target for preserving retinal function in aging and disease. - Source: PubMed
Becerra S Patricia