P2RX4 antibody (Biotin)
- Known as:
- P2RX4 (anti-) (Biotin)
- Catalog number:
- orb114302
- Product Quantity:
- 100 ul
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- P2RX4 antibody (Biotin)
Related genes to: P2RX4 antibody (Biotin)
- Gene:
- P2RX4 NIH gene
- Name:
- purinergic receptor P2X 4
- Previous symbol:
- -
- Synonyms:
- P2X4
- Chromosome:
- 12q24.31
- Locus Type:
- gene with protein product
- Date approved:
- 1997-10-09
- Date modifiied:
- 2016-10-05
Related products to: P2RX4 antibody (Biotin)
Related articles to: P2RX4 antibody (Biotin)
- The P2RX7 gene has been linked to various neuropsychiatric disorders. In particular, the SNP rs2230912, which results in a glutamine-to-arginine substitution at position 460, has repeatedly been associated with mood disorders. Although this SNP per se does not affect receptor function, it tags a gain-of-function haplotype that has been shown to significantly enhance receptor activity. BAC-transgenic P2X7-reporter mice have proven to be valuable tools for monitoring P2X7 expression. Here, we exploited their capacity to simultaneously overexpress the receptor, thereby serving as gain-of-function models to assess the behavioral consequences of elevated P2X7 levels. We used the two currently available transgenic P2X7 reporter lines (sEGFP and P2X7-EGFP), which differ in their expression pattern, degree of overexpression, and co-expression of the neighbouring P2rx4 gene. Male sEGFP and P2X7-EGFP mice showed no alterations in general activity or in measures of anxiety-related or stress-coping behavior compared to their wild-type littermates. Only male P2X7-EGFP mice exhibited slightly delayed locomotor habituation to a novel environment. To what extent higher expression levels reflect enhanced receptor activity, as conveyed by the disease-associated gain-of-function haplotype, requires further investigation. Overall, these results indicate that P2X7 overexpression-whether at endogenous or ectopic sites, or in conjunction with P2X4-is not sufficient to substantially alter behavior of individually housed male mice under baseline conditions. - Source: PubMed
Publication date: 2026/05/09
Urbina-Treviño LidiaTang Haovon Mücke-Heim Iven-AlexEngel TobiasNicke AnnetteDeussing Jan M - P2 purinergic receptors are activated by extracellular adenosine triphosphate and other nucleotides released during inflammatory processes, cellular stress responses, and amplification by NETosis, thereby serving as pivotal mediators of both innate and adaptive immunity. In patients with active systemic lupus erythematosus (SLE), emerging evidence highlights the critical roles of distinct P2 receptors: P2RX4 and P2RY11 in initiating the immune response; P2RY2 in orchestrating immune cell recruitment; P2RX7 in promoting pro-inflammatory states coupled with impaired regulatory mechanisms; and P2RY12 as a driver of type I interferon signaling. Therapeutic targeting of these receptors through selective antagonists has demonstrated efficacy in preclinical lupus-prone models to restore regulatory functions (P2RX7), to control inflammation (P2RX7), type I interferon pathway (P2RY12), autoantibody production (P2RX4 and P2RX7), and glomerulonephritis (P2RX4, P2RX7, P2RY2, and P2RY12). In SLE, selective P2 antagonists are under investigation with major challenges regarding cellular specificity, therapeutic efficacy, and side effects. - Source: PubMed
Publication date: 2026/03/12
Renaudineau YvesBrooks Wesley - Early detection of hepatocellular carcinoma (HCC) remains a persistent worldwide challenge. Owing to its minimal invasiveness, liquid biopsy has emerged as a promising alternative for early screening. As key components of the tumor microenvironment (TME), platelets (PLTs) represent a rich source of biomolecular information that complements the data from conventional plasma and serum samples. Integrative multiomics analysis of such data offers a powerful strategy to deepen our understanding of hepatocarcinogenesis and accelerate the discovery of robust biomarker panels. Here, we described an integrative and ultrafast multiomics sample preparation (IAU-MOSP) strategy for the high-purity platelets. The optimized IAU-MOSP method shortened the multiomics workflow from over 24 to 6 h while yielding comparable biomolecule identifications to those of conventional methods. Then, the workflow was applied in an HCC cohort ( = 68) study. We quantified 6660 biomolecules with high reproducibility (median CVs: 0.31-0.39). The data exhibited strong cross-omics correlations, particularly between proteins and lipids ( = 0.75) as well as protein and metabolite ( = 0.68) groups. Differential analysis revealed 10 biomolecules significantly dysregulated in HCC platelets (TEK, citric acid, glycerol-3-phosphate (G3P), P2RX4, malic acid, ATP, PRG3, ITGAM, CXCR2, ITGB2) that participate in key pathways driving proliferation and metastasis. Accompanied by machine learning, the 10 biomolecules were ultimately identified as a potential biomarker panel for early diagnosis of HCC. It shows superior diagnostic efficacy (accuracy = 0.81, sensitivity = 0.74) over α-fetoprotein (AFP) (accuracy = 0.75, sensitivity = 0.45) for early HCC detection. - Source: PubMed
Publication date: 2026/03/10
Shen FenglinLiu YangRuan XuelianYan GuoquanZhang LeiFang JingLu HaojieHu ZuojianZhou Xinwen - Although FDA-approved medications for alcohol use disorder are available, their efficacy varies across patients, highlighting the need for novel therapeutics that address inter-individual differences in disease etiology and treatment response. Genetic models, particularly heterogeneous stock (HS) rats, recapitulate human-like genetic diversity and behavioral heterogeneity, enabling the dissection of individual differences in vulnerability to AUD and pharmacotherapeutic sensitivity. P2X4 receptors, which are encoded by the gene P2rx4, are ATP-gated ion channels are inhibited by ethanol and abundantly expressed in neurons found in reward and stress circuits. P2X4 receptors have emerged as key modulators of ethanol sensitivity and consumption in preclinical models. Here, we genetically predicted P2rx4 expression in whole brain in 131 male and female HS rats exposed to chronic intermittent ethanol vapor and phenotyped for self-administration during acute abstinence. Rats were dichotomized into genetically predicted high and low expression groups. We found that higher genetically predicted P2rx4 expression was associated with increased post-vapor intake and escalation. In 32 CIE-escalated rats, ivermectin, a positive allosteric modulator of P2X4 receptors, dose-dependently reduced drinking. We stratified rats into three groups: non-responders, mild responders, and high responders. Electrophysiological recordings from CeA slices revealed that ivermectin differentially enhanced GABAergic IPSCs: high-responders exhibited sustained increases in IPSC frequency and selective amplitude reductions, while the two other groups showed transient frequency increases. All groups displayed prolonged rise times, however non-responders showed extended decay times. These findings suggest that P2rx4 upregulation serves as a vulnerability marker for dependence-like behaviors, with ivermectin attenuating withdrawal-driven alcohol consumption by enhancing CeA GABAergic inhibition. - Source: PubMed
Publication date: 2026/02/12
Campo PaolaQiao RanDoyle Michelle RMunro DanielJohnson Benjamin BPalmer Abraham AKallupi Marsidade Guglielmo Giordano - Although FDA-approved medications for alcohol use disorder (AUD) are available, their efficacy varies across patients, highlighting the need for novel therapeutics that address interindividual differences in disease etiology and treatment response. Genetic models, particularly heterogeneous stock (HS) rats, recapitulate human-like genetic diversity and behavioral heterogeneity, enabling the dissection of individual differences in vulnerability to AUD and pharmacotherapeutic sensitivity. P2X4 receptors, which are encoded by the gene , are ATP-gated ion channels inhibited by ethanol and abundantly expressed in neurons found in reward and stress circuits. P2X4 receptors have emerged as key modulators of ethanol sensitivity and consumption in preclinical models. Here, we genetically predicted expression in whole brain in a cohort of 130 HS rats exposed to chronic intermittent ethanol (CIE) vapor and phenotyped for self-administration during acute abstinence. Rats were dichotomized into high- and low-predicted expression groups. Higher predicted expression was associated with increased post-vapor intake and escalation. In 32 CIE-escalated rats, ivermectin, a positive allosteric modulator of P2X4 receptors, dose-dependently reduced drinking. We stratified rats into three groups: non-responders, mild responders, and high responders. Electrophysiological recordings from CeA slices revealed that ivermectin differentially enhanced GABAergic IPSCs: high-responders exhibited sustained increases in IPSC frequency and selective amplitude reductions, while the two other groups showed transient frequency increases. All groups displayed prolonged rise times, however non-responders showed extended decay times. These findings suggest that upregulation serves as a vulnerability marker for dependence-like behaviors, with ivermectin attenuating withdrawal-driven alcohol consumption by enhancing CeA GABAergic inhibition. - Source: PubMed
Publication date: 2025/10/15
Campo PaolaQiao RanDoyle Michelle RMunro DanielJohnson Benjamin JPalmer Abraham AKallupi Marsidade Guglielmo Giordano