GJA3 antibody
- Known as:
- GJA3 (anti-)
- Catalog number:
- orb128111
- Product Quantity:
- 200 ug
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- GJA3 antibody
Related genes to: GJA3 antibody
- Gene:
- GJA3 NIH gene
- Name:
- gap junction protein alpha 3
- Previous symbol:
- CZP3
- Synonyms:
- CX46
- Chromosome:
- 13q12.11
- Locus Type:
- gene with protein product
- Date approved:
- 1990-02-12
- Date modifiied:
- 2015-11-09
Related products to: GJA3 antibody
Related articles to: GJA3 antibody
- Age-related cataract (ARC) is a degenerative change following the aging of the lens, with its specific pathogenesis still unclear. This study investigates the expression of GSK-3β, DNMT3B, and GJA3 in ARC through in vivo and in vitro studies, elucidating the regulatory mechanisms among them. Additionally, it preliminarily explores the role of DNMT3B in the epigallocatechin gallate (EGCG)-mediated delay of apoptosis in lens epithelial cells (LECs). Immunohistochemistry and Western blot results showed that the protein expression level of DNMT3B was elevated, while the protein expression levels of GJA3 and p-GSK-3β (Ser9) were decreased in the ARC group and the aged mouse group (P < 0.05). CCK-8 and Hoechst 333,42 apoptosis assays indicated that DNMT3B, GJA3, and p-GSK-3β (Ser9) were involved in HO-induced apoptosis of LECs. Further investigation revealed that DNMT3B regulates lens epithelial cell apoptosis by modulating GJA3 expression, and the expression of DNMT3B is regulated by GSK-3β. Additionally, we found that EGCG effectively alleviates DNMT3B-mediated apoptosis of LECs (P < 0.05). In summary, our study indicates that p-GSK-3β, DNMT3B, and GJA3 play significant roles in ARC and oxidative stress-induced apoptosis of LECs, with interrelated regulatory mechanisms. EGCG can target DNMT3B to reduce apoptosis in LECs, offering a new direction for the development of cataract treatments. - Source: PubMed
Publication date: 2026/06/18
Chen XiaoyaSu DongmeiZhang GaoboXiang ZihanFu YanjiangSun ZhaoyiHu YuzhuLi SijiaZhang YueMa XuHu ShanshanWei Qianqiu - Posterior polar cataract (PPC), a rare congenital cataract, is characterized by subcapsular opacities in the lens affecting vision and posing surgical challenges. Genetic heterogeneity exists, though several genes are implicated in its pathogenesis. This study reports on the genetic profile of late-onset PPC cases and identifies variations specific to posterior capsular rupture (PCR) during surgery. - Source: PubMed
Publication date: 2026/02/11
Sharma AnshulSharma NamrataAgarwal TusharSharma ArundhatiVajpayee Rasik B - Congenital cataract (CC), defined as lens opacity present at birth or in early infancy, is a major cause of reversible childhood blindness and shows marked genetic heterogeneity. This study aimed to investigate the genetic basis of CC in a multigenerational Chinese family. - Source: PubMed
Publication date: 2026/01/13
Zhou ChenchenLi KunkeZhou ZhenxingJian ShuhuiJin LingWang ChenghuZhang Xiaoqian - Gap junctions, formed by connexin proteins, establish direct electrical and metabolic coupling between cells, enabling coordinated tissue responses. These channels universally respond to intracellular pH changes, closing under acidic conditions to limit the spread of cytotoxic signals during cellular stress, such as ischemia. Using cryo-electron microscopy (cryo-EM), we uncover insights into the structural mechanism of pH-gating in native lens connexin-46/50 (Cx46/50) gap junctions. Mild acidification drives lipid infiltration into the channel pore, displacing the N-terminal (NT) domain and stabilizing pore closure. Lipid involvement is shown to be both essential and fully reversible. Structural transitions involve an ensemble of gated states formed through non-cooperative NT domain movement as well as minor populations of a distinct destabilized open-state. These findings provide molecular insights into pH-gating dynamics, illustrating how structural changes may regulate gap junction function under cellular stress and linking Cx46/50 dysregulation to age-related cataract formation. - Source: PubMed
Publication date: 2026/01/12
Jarodsky Joshua MMyers Janette BReichow Steve L - Drought and water scarcity, exacerbated by global warming, are enormous threats to global food sustainability and security. Poultry, in particular, are highly impacted by adverse environmental stressors. As nutrient absorption and intestinal integrity are critical for growth and performance, understanding the impact on the broiler gastrointestinal tract is highly relevant. Here, we examined the effect of chronic cyclic heat stress (HS) on the jejunal expression profile of tight-junction, gap-junction, adherens, and desmosome genes in the 4th generation of broiler lines divergently selected for low (LWE)- and high-water efficiency (HWE). Male HWE and LWE broilers (n = 240/line) were allotted to 12 environmental chambers (2 floor pens/chamber, 6 chambers/line, 20 birds/pen) and were exposed to cyclic HS (36°C for 9h/day from 9:00 am to 6:00 pm) or thermoneutral conditions (25°C) from day 29 to 49 of age in a 2 × 2 factorial design. Growth performance and mortality were recorded. At day 49, jejunal tissues were collected for molecular analyses using real-time quantitative PCR and immunoblot. Jejunal gene expression of multiple gut integrity factors were higher (P < 0.05) in the HWE as compared to the LWE lines, including claudin 22 (CLDN22), -34, occluding (OCDN), zona-occludin-2 (ZO-2), gap junction alpha1 (GJA1), GJA3, GJC1, and cadherin 1 (CDH1). CLDN8, -20, -25, -4, GJC2, and GJD2 were also greater (P < 0.05) in HWE, but were additionally downregulated (P < 0.05) during HS. Conversely PALS1-associated tight junction protein (PATJ) and desmocollin 1 (DSC1) mRNAs were significantly downregulated in the HWE as compared to the LWE broilers. Significant interactions between the line and environment were seen in CLDN1, where the expression was decreased in the LWE but increased in the HWE in HS. Additionally, CLDN15 and -16 genes were greatest in the HWE under TN conditions, while catenin alpha 2 (CTNNA2) was highest in the HWE during HS. Overall, the jejunal expression profile of key genes associated with intestinal barrier integrity likely contributes to the water efficiency phenotype and the response of these birds to HS. - Source: PubMed
Publication date: 2025/12/16
Greene Elizabeth SOrlowski SaraDridi Sami