MOV10L1 antibody
- Known as:
- MOV10L1 (anti-)
- Catalog number:
- orb125972
- Product Quantity:
- 200 ug
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- MOV10L1 antibody
Related genes to: MOV10L1 antibody
- Gene:
- MOV10L1 NIH gene
- Name:
- Mov10 like RISC complex RNA helicase 1
- Previous symbol:
- -
- Synonyms:
- DJ402G11.8, DKFZp434B0717, CHAMP
- Chromosome:
- 22q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 2000-05-31
- Date modifiied:
- 2018-06-07
Related products to: MOV10L1 antibody
Related articles to: MOV10L1 antibody
- Highly pathogenic avian influenza viruses (HPAIVs) of the H5N8 subtype have caused severe outbreaks in poultry and wild birds worldwide. Disease outcome differs markedly between avian species: chickens typically develop acute infection with high mortality, whereas ducks often exhibit milder or delayed disease. The molecular basis underlying these species-specific differences in early host responses remains incompletely understood. To characterize early transcriptional responses to HPAIV infection, RNA sequencing (RNA-seq) was performed on lung tissues from chickens and ducks experimentally infected with H5N8 HPAIV at 24 hours post-inoculation (hpi). Differential gene expression analysis and functional enrichment analyses were conducted, and selected host genes were validated by quantitative real-time PCR. At 24 hpi, a comparable number of differentially expressed genes (DEGs) was identified in chickens and ducks; however, the organization of the transcriptional response differed markedly between species. Chickens exhibited a pathway-centered transcriptional profile dominated by innate immune and inflammatory signaling, whereas ducks displayed a more distributed and balanced transcriptional response spanning multiple functional categories. Functional enrichment analyses revealed strong activation of interferon- and cytokine-associated pathways in chickens, while transcriptional changes in ducks were dispersed across processes related to RNA metabolism, antiviral regulation, and cellular homeostasis. Among species-specific differentially expressed genes, MOV10L1 showed opposite regulation in these bird species. These findings demonstrate that early host responses to H5N8 HPAIV infection differ between chickens and ducks not only in gene identity but also in transcriptional organization. The distributed response architecture observed in ducks may contribute to controlled antiviral responses with limited immunopathology. This study provides a comparative transcriptomic framework for understanding species-specific host responses to HPAIV infection in poultry. - Source: PubMed
Publication date: 2026/06/11
Sajewicz-Krukowska JoannaDomańska-Blicharz KatarzynaTarasiuk KarolinaŚmietanka KrzysztofMarzec-Kotarska Barbara - Spermatogenesis is a complex differentiation process requiring precise spatiotemporal gene regulation, yet the functions of most testis-enriched long noncoding RNAs (lncRNAs) remain elusive. Here, we identify as a pachytene-specific lncRNA that is essential for mouse spermatogenesis. Reanalysis of single-cell RNA-seq reveals that expression is minimal in spermatogonia and preleptotene cells, sharply increases during the pachytene stage, and declines after meiosis, precisely coincides with the wave of pachytene piRNA production. In vivo knockdown of in mouse testes via AAV9-shRNA severely disrupts spermatogenesis, causing a reduction in testis size, seminiferous epithelium thickness, and epididymal sperm count, suggesting its physiological necessity. Mechanistically, we demonstrate that functions as a bona fide piRNA precursor: it harbors numerous piRNA sequences, and its overexpression in GC-2 spd(ts) cells specifically upregulates corresponding overlapping piRNAs. This processing relies on the core piRNA machinery, and co-overexpression of , together with or , promotes piRNA production. Notably, this piRNA precursor function is evolutionarily conserved, as the human homolog , which is linked to asthenozoospermia, also generates piRNAs when expressed in GC-2 spd(ts) cells. Transcriptomic analysis following overexpression in GC-2 spd(ts) cells reveals dysregulation of genes involved in metabolism and signaling, with several spermatogenesis-related genes (e.g., , ) predicted as direct targets of -derived piRNAs. Our study identifies as a pachytene-specific lncRNA essential for male fertility and demonstrates that it functions as a conserved piRNA precursor. These findings provide mechanistic insight into how an individual lncRNA contributes to the piRNA pathway and suggest that it may operate through dual mechanisms in mammalian spermatogenesis. - Source: PubMed
Publication date: 2026/05/11
Jian ShikunHan XuXie ChenyaoMa YinghaoChen PeizeLiu WeiguangXiao JunSong Xiaoyuan - Pachytene piRNAs are the least understood class of piRNAs in the mammalian male germ line. During meiosis, their biogenesis occurs near the mitochondrial outer membrane in germ granules known as intermitochondrial cement (IMC). However, how mitochondrial factors regulate the trafficking of PIWI proteins into and out of the IMC remain poorly understood. Here we show that the cytoplasmic PIWI proteins MILI and MIWI are recruited for pachytene piRNA biogenesis via distinct mitochondrial membrane proteins. Loss of the mitochondrial scaffold protein ASZ1 during meiosis in mice disrupts multiple downstream biogenesis steps, resulting in misregulation of MILI, MIWI, and MOV10L1, failure of IMC formation, and an almost complete loss of mature pachytene piRNAs. Strikingly, despite the drastic depletion of pachytene piRNAs, LINE1 transposon silencing remains unaffected. We identify three classes of pachytene piRNA pathway components that coordinate piRNA production and compartmentalization. Our findings reveal that chromatoid body precursors serve as a central hub for the accumulation of pachytene PIWI-piRNA complexes, thus establishing a connection between IMC-based biogenesis and downstream piRNA function. - Source: PubMed
Publication date: 2026/05/12
Yan XiaoyuanWei ChaoMann Jeffrey MShang GuanyiWang QianyiXie HuirongDemireva Elena YSun LiangliangDing DeqiangChen Chen - The formation of biomolecular condensates, such as Yb bodies in Drosophila ovarian somatic cells, is significantly contributed to by phase separation of scaffold proteins. Client proteins transiently accumulate via interactions with these scaffolds; however, how client flux is regulated remains unclear. Here, we investigate Shutdown, a client protein of Yb bodies-the site of Piwi-piRNA-induced silencing complex (piRISC) precursor (pre-Piwi-piRISC) formation-and show that cytosolic Shutdown connects Armitage and Piwi to promote Piwi deposition into Yb bodies before quickly returning to the cytosol. This return allows Armitage to transfer pre-Piwi-piRISC to mitochondria for maturation. Shutdown's acidic N terminus mediates self-repulsion, conferring its transient residence within Yb bodies. A point mutation in armitage, analogous to a human armitage/MOV10L1 mutation associated with azoospermia, traps Shutdown in Yb bodies, blocking Piwi-piRISC generation. This study reveals the mechanism underlying Shutdown's transient localization in Yb bodies and its essential role in Piwi-piRISC biogenesis and fertility. - Source: PubMed
Hirakata ShigekiFukaya TakashiFujita AoiKosako HidetakaSiomi Mikiko C - Splenic nodules in dogs that were historically classified under the broad term "fibrohistiocytic nodules" are now recognised as distinct entities within likely a biological continuum. These include lymphoid hyperplasia extending to indolent lymphoma and complex hyperplasia to stromal sarcoma. However, the molecular mechanisms underpinning these proposed progressions remain largely unexplored, particularly at the genomic and transcriptomic levels. This study aimed to delineate and compare the transcriptomic landscapes of four distinct canine splenic nodules through differential gene expression profiling. RNA sequencing was performed on twelve formalin-fixed, paraffin-embedded (FFPE) splenic tissue samples obtained from dogs diagnosed with lymphoid hyperplasia, complex hyperplasia, histiocytic sarcoma, and stromal sarcoma, with normal canine spleen serving as a control tissue. Comparative transcriptomic analysis identified 47 differentially expressed genes (DEGs) between splenic nodules and normal spleen, including , , , , , , , , , , and . Furthermore, 39 DEGs were significantly altered among the four splenic lesion types, such as , , , , , , , , , , , , , and . Many of these genes have previously been implicated in tumorigenesis and metastasis in other malignancies. These findings suggest that dysregulated gene expression may contribute to the activation of stromal cells and macrophages within the spleen, facilitating malignant transformation. Overall, these findings deliver novel transcriptomic insights into canine splenic tumorigenesis that may improve diagnostic precision, inform prognostic assessment, and support the development of targeted therapeutic strategies in veterinary oncology. - Source: PubMed
Publication date: 2026/01/29
Spröhnle-Barrera CleideAllavena RachelPalmieri Chiara