TFAP2A antibody
- Known as:
- TFAP2A (anti-)
- Catalog number:
- orb33549
- Product Quantity:
- 20 ug(Trial size)
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- TFAP2A antibody
Related genes to: TFAP2A antibody
- Gene:
- TFAP2A NIH gene
- Name:
- transcription factor AP-2 alpha
- Previous symbol:
- TFAP2, AP2TF
- Synonyms:
- AP-2
- Chromosome:
- 6p24.3
- Locus Type:
- gene with protein product
- Date approved:
- 1991-09-12
- Date modifiied:
- 2016-04-06
Related products to: TFAP2A antibody
Related articles to: TFAP2A antibody
- In the whole laying cycle, the late laying phase is the longest segment, and it directly determines total egg production and influences the timing of culling. To investigate the mechanisms underlying the decline in egg-laying performance during this phase, our study selected Wenchang chickens as the research subjects, which are characterized by low egg-laying rates and high phenotypic variation. We investigated egg-laying patterns in 872 Wenchang chickens during the late laying phase and performed mechanistic studies on three high egg-laying (laying rates > 76.0 %) hens and three low egg-laying hens (laying rates < 50.0 %). RNA-seq and ATAC-seq analyses were conducted on ovarian stroma tissues from these hens. RNA-seq analysis identified 800 differentially expressed genes with thresholds of p < 0.05 and |log₂FC| ≥ 1, including 317 upregulated and 483 downregulated genes, which were enriched in biological processes such as immune response, negative regulation of endopeptidase activity, tight junction, and neuroactive ligand-receptor interaction. ATAC-seq revealed 1,065 and 428 candidate differentially accessible regions in the two groups, respectively, with 9.8 % and 5.8 % of these regions located in promoter regions. Integrated analysis identified three key genes potentially influencing the laying performance in the late phase, including MICA, MHCIA6 and MHCIY. These genes all belong to the non-classical MHC-Ⅰ family and are implicated in immune regulation. Additionally, four transcription factors (HOXB6, GATA3, TFAP2A, and WT1) were predicted to regulate these key genes. Collectively, our findings suggest that an imbalance in ovarian immune homeostasis during the late laying phase may be a significant driver of the decline in egg production. This study not only provides mechanistic insights into the maintenance of high egg-laying performance but also offers potential molecular targets for genetic improvement in Wenchang chickens and advances the understanding of reproductive regulation in poultry. - Source: PubMed
Publication date: 2026/05/10
Yang HongruiWang LeiRong LiNan JiuhongCai JinpingZhao YunxiaZhu GuiyuLi Shijun - Cranial neural crest (CNC) cells are a key stem cell like tissue that contribute to most of the facial structures in vertebrates. A disintegrin and metalloproteinase (ADAM) family of proteins is essential for the induction and migration of the CNC. We have shown that Adam13 interacts with the transcription factor Arid3a to regulate gene expression; we show that Adam13 modulates histone modifications in the CNC and that Arid3a binding to the promoter is dependent on the presence of Adam13. These associations promote the expression of a certain variant expressed in the CNC that uniquely activates the expression of genes critical to CNC migration. Furthermore, we show that both Adam13 and human ADAM9 are associated with proteins involved in histone modifications and RNA splicing (a function critically affected by the loss of Adam13). Thus, we propose that ADAMs may act as extracellular sensors to modulate chromatin availability, leading to changes in gene expression and splicing. - Source: PubMed
Publication date: 2026/05/07
Pandey AnkitCousin HeleneKumar ShivTaylor LouisChander AshmitaCoppenrath KelseyShaidani Nikko-IdeenHorb MarkoAlfandari Dominique - Clear cell renal cell carcinoma (ccRCC) is the most common renal carcinoma subtype. Aging-related genes (ARGs) are implicated in ccRCC progression, though their mechanisms remain unclear. This study aimed to elucidate the molecular mechanisms of ARGs in ccRCC and identify potential prognostic biomarkers and therapeutic targets. Transcriptomic data from the cancer genome atlas (TCGA-ccRCC) cohort were analyzed. differentially expressed genes and ARGs were intersected to identify candidate genes. Prognostic ARGs were then screened using machine learning algorithms. A risk model was constructed and validated through survival analysis, stratifying patients into high- and low-risk groups. Functional enrichment, immune infiltration, and drug prediction analyses were performed. The expression levels of prognostic genes in ccRCC tissues were validated through reverse transcription quantitative polymerase chain reaction (RT-qPCR). A total of 2312 differentially expressed genes and 307 ARGs were identified, of which 25 overlapping genes were selected as candidates. Seven ARGs were significantly associated with patient prognosis: protective PCK1, and risk-related TOP2A, TFAP2A, CCNA2, FOXM1, CDKN2A, and PLAU. High-risk patients showed reduced overall survival rates. Immune infiltration and checkpoint expression differed significantly between risk groups. decision curve analysis indicated high clinical utility. Drug prediction identified 69 potential therapeutic compounds, including tyrosine kinase and mTOR inhibitors. RT-qPCR validated 5 genes' expression, consistent with bioinformatics predictions, though discrepancies were observed in the expression patterns of FOXM1 and CDKN2A. Seven ARGs were identified as key prognostic markers in ccRCC. A robust risk model was established, providing insights into ARG-related mechanisms and potential diagnostic and therapeutic strategies. - Source: PubMed
Liu ChenShuang WeibingCao XiaomingGao Ying - The oncogenic roles of microRNAs have been extensively documented across various cancer types. In the present study, we aimed to investigate the functional impact of miR-135a-5p on the clear cell renal cell carcinoma (ccRCC) cell line 769P. Through weighted gene co-expression network analysis (WGCNA), we identified a significant association between miR-135a-5p and ccRCC progression. Furthermore, miR-135a-5p expression was downregulated in both ccRCC tissues and cell lines. Functional assays demonstrated that miR-135a-5p inhibits the progression of 769P cells. Mechanistically, miR-135a-5p directly binds to the 3' untranslated region (3'UTR) of TFAP2A, reducing its mRNA stability. In turn, TFAP2A binds to the promoter region of KRT8 and activates its transcription, thereby mediating the inhibitory effect of miR-135a-5p on 769P cell progression. In conclusion, our findings provide evidence that miR-135a-5p suppresses ccRCC progression in 769P cells through the TFAP2A/KRT8 axis, highlighting its potential as a therapeutic target for ccRCC. - Source: PubMed
Publication date: 2026/05/08
Xie DelongZhou JizheGuo YueyueWan GuangyangLu FeiyanGuo YuyingYi SanguiLiu Zongling - Long-term exposure to low-dose food contact materials (FCMs) has raised concerns regarding developmental toxicity. In the present study, we prioritized FCMs with potential developmental toxicity using a weight-of-evidence computational model, which predicted 127 chemicals to be of high concern. From these, we selected 7 chemicals-representing both high and low concern categories-and evaluated their potential embryotoxicity using the mouse embryonic stem cell test (mEST). Among the selected chemicals, thiram most strongly inhibited cardiac differentiation in mEST. We further examined the effects of thiram on morphology and expression of differentiation-related genes in mouse embryonic stem cells (mESCs). Treatment with thiram inhibited 50% early differentiation in mESCs, suppressed the expression of markers associated with the three-germ layers, but increased the expression of neurectoderm markers during early embryogenesis. Additionally, treatment with 20 ng/mL thiram, which was the lowest-observed-effect concentration of cytotoxicity, disrupted neuronal differentiation in both mESCs and human pluripotent embryonal carcinoma NT2 cells. Finally, based on transcriptome analysis, 20 and 30 ng/mL thiram disrupted the neural crest differentiation pathway, altering the expression of genes including homeobox A1 (HOXA1), homeobox B1 (HOXB1), Heart And Neural Crest Derivatives Expressed 1 (HAND1), Distal-Less Homeobox 5 (DLX5), and transcription factor AP-2 alpha (TFAP2A) in NT2 cells. Therefore, disruption of neural crest differentiation is one of the potential mechanisms underlying thiram-induced embryotoxicity. The integrated alternative approach adopted in the present study to identify mechanism-based biomarkers for thiram-induced embryotoxicity in a human-relevant model could facilitate safety assessment for data-poor chemicals in future. - Source: PubMed
Publication date: 2026/04/21
Ho Chia-ChiTung Chun-WeiYen B LinjuWeng Chen-YiHsu Ju-HsinTsai Ming-HsienArrokhman SalimLin Pinpin