DISC POLYETH 20MU PKG 100
- Known as:
- DISC POLYETH 20MU PKG 100
- Catalog number:
- 37000-1
- Product Quantity:
- Case of 100
- Category:
- -
- Supplier:
- COREX
- Gene target:
- DISC POLYETH 20MU PKG 100
Related genes to: DISC POLYETH 20MU PKG 100
- Gene:
- DISC2 NIH gene
- Name:
- disrupted in schizophrenia 2
- Previous symbol:
- -
- Synonyms:
- DISC1-AS1, DISC1OS, NCRNA00015
- Chromosome:
- 1q42.1
- Locus Type:
- RNA, long non-coding
- Date approved:
- 2000-01-20
- Date modifiied:
- 2018-05-16
Related products to: DISC POLYETH 20MU PKG 100
Related articles to: DISC POLYETH 20MU PKG 100
- Human induced pluripotent stem cells (hiPSCs) are powerful tools for disease modeling and therapeutic development, though their utility remains sensitive to operator-dependent variability and media formulation. Commercial stem cell media formulations, such as mTeSR+ and StemFlex, are designed to support robust pluripotent stem cell maintenance and improve reproducibility. While both are widely used, direct comparisons are limited. We evaluated mTeSR+ and StemFlex using the CellXpress.ai automated tissue culture platform in combination with IN Carta machine-learning-based image analysis to monitor and standardize feeding, passaging, and maintenance. Wild-type iP11N hiPSCs were cultured under standardized automated conditions with growth kinetics, immunocytochemical marker expression, and RT-qPCR assessment. Automated analysis revealed no significant differences in proliferation rates between the formulations; however, longitudinal image-based quantification identified statistically significant differences (<1%) in differentiated cell area fraction across the imaging period. Immunocytochemistry demonstrated comparable expression and localization of pluripotency-associated markers, and RT-qPCR analysis confirmed similar expression of stemness factors. These findings demonstrate that both media support comparable growth and maintenance of pluripotency under standardized automated conditions on iP11N wild-type stem cells in the short-term culture conditions analyzed. Additionally, these data highlight the value of automated imaging and machine-learning-based analysis in reducing operator-dependent variability and improving consistency in hiPSC culture, supporting its application in high-throughput stem cell research. - Source: PubMed
Publication date: 2026/08/25
Bogoniewski Aleksander AGonzalez Melissa FReyes Rachel ERivera Susanna FTaylor CameronSchurr ToddDamoiseaux RobertLipshutz Gerald S - Lipid nanoparticles (LNPs) have great potential as nucleic acid delivery vehicles; however, they trigger the production of inflammatory cytokines, which limits their medical applications. Developing non-inflammatory LNPs is challenging because the LNP's ionizable lipid and the process of endosomal disruption are the major sources of LNP toxicity but are also essential for delivering nucleic acids. Here we demonstrate that ionizable lipids containing a carboxylic acid and an amine (termed S-lipid) switch their charged state between the pHs of 7.4 and 4.0, allowing them to generate LNPs (termed switchable nanoparticles) that efficiently encapsulate nucleic acid and trigger endosomal release without activation of the TLR4, complement, galectin-8 and platelet activating factor signalling pathways. Finally, we demonstrate that switchable nanoparticles are better at treating lipopolysaccharide-induced acute lung injury than traditional LNPs because they do not exacerbate pre-existing inflammation. Collectively, these results demonstrate that negatively charged ionizable lipids can mitigate the toxicity of LNPs. - Source: PubMed
Publication date: 2026/09/08
Liang DengpanQi YalinHan HesongAhmadian NegarGao KewaSapasap KayceeZhang YuxiGuo SilinLawanprasert AtipPimcharoen SopidaZhao ShengDel Buono Milan TXia HeEnders Zoe OBurgstone Benjamin WCalio AntoninoDankar NeelLu BingweiQi Lei SWang AijunMurthy Niren - Radiation therapy is a standard-of-care oncological treatment for central nervous system (CNS) malignancies. However, as survival outcomes improve, radiation-induced injury to normal brain tissue has increased in clinical significance. CNS radiation injury is a delayed, multifactorial process characterized by impaired neurogenesis, reactive gliosis, and persistent functional deficits. Mechanistic exploration and development of effective radiation mitigators have been limited by the lack of scalable, human-relevant models. - Source: PubMed
He LingKornblum Harley IBhaduri AparnaPajonk Frank - V2a interneurons are excitatory neurons found throughout the hindbrain and spinal cord, two regions that arise from distinct progenitors during embryonic development. Whether this lineage difference shapes mature gene regulation and function is unknown. V2a neurons show plasticity after spinal cord injury and are candidates for cell therapy. We differentiated human stem cells into V2a neurons through hindbrain- and spinal cord-like progenitor routes and profiled them by single nucleus multiomic sequencing. The two lineages showed distinct transcription factor motif enrichment and differentially expressed genes governing axon growth and calcium handling. Inducing V2a transcription factors directly, bypassing developmental patterning, produced a population unlike either lineage, confirming progenitor history is not interchangeable. Using CellOracle and lentiviral knockdown, we identified CREB5 and TCF7L2 as regulators specific to the spinal-like lineage. These results show that progenitor origin shapes V2a identity and reveal new regulators of neural diversity along the anterior-posterior axis. - Source: PubMed
Publication date: 2026/07/29
Elder Nicholas HMajd AlirezaCesiulis AndriusNyarady SpencerSankar KalyanBulger Emily ASamuel Ryan MZholudeva Lyandysha VMcDevitt Todd CFattahi Faranak - Presynaptic congenital myasthenic syndromes (CMS) encompass a large number of rare neurologic disorders caused by impaired release of acetylcholine (ACh) from motor nerve terminals. There are two main groups of presynaptic CMS: one in which the amount of ACh in synaptic vesicles (SV) is diminished and another in which the mechanism of synaptic vesicle release is impaired. The latter is often referred to as a congenital Lambert-Eaton myasthenic syndrome (LEMS). Presynaptic CMS, like other forms of CMS, is primarily characterized by muscle weakness and fatigue, but is often associated with additional manifestations of central and autonomic nervous system involvement, including episodic apneas, cognitive delay, seizures, and gastrointestinal complications. The most common causes of presynaptic CMS are recessive and dominant mutations in genes encoding proteins participating in the mechanisms of ACh synthesis and SV release. However, at times gene mutations can result in more complex pathogenic mechanisms. The most effective treatment of these conditions is prevention of potentially fatal episodes of apnea and other respiratory complications resulting from bulbar weakness. Pharmacologic treatments are also useful; however, in many cases the response is incomplete, and in other mild cases there is improvement with age that obviates the need for pharmacologic interventions. Preclinical studies in animal models of presynaptic CMS using targeted gene therapies are promising but these new therapies will not be available in the near future. - Source: PubMed
Publication date: 2026/07/01
Maselli Ricardo A