MKK6 Antibody (Ab-207), pAb, Rabbit
- Known as:
- MKK6 Antibody (Antibody-207), pAb, Rabbit
- Catalog number:
- A00438-100
- Product Quantity:
- 100,0μg
- Category:
- -
- Supplier:
- Genscript
- Gene target:
- MKK6 Antibody (Ab-207) pAb Rabbit
Related genes to: MKK6 Antibody (Ab-207), pAb, Rabbit
- Gene:
- MAP2K6 NIH gene
- Name:
- mitogen-activated protein kinase kinase 6
- Previous symbol:
- PRKMK6
- Synonyms:
- MEK6, MKK6, SAPKK3, MAPKK6
- Chromosome:
- 17q24.3
- Locus Type:
- gene with protein product
- Date approved:
- 1997-11-11
- Date modifiied:
- 2016-01-15
Related products to: MKK6 Antibody (Ab-207), pAb, Rabbit
Related articles to: MKK6 Antibody (Ab-207), pAb, Rabbit
- Osteoarthritis (OA) is a chronic degenerative joint disease characterized by progressive articular cartilage degradation. Punicic acid (PA), a plant-derived n-3 polyunsaturated fatty acid, has been reported to exert anti-inflammatory and anti-apoptotic effects. However, the molecular mechanisms underlying its effects in OA remain incompletely understood. In this study, bioinformatics analysis was performed to identify apoptosis-related differentially expressed genes (DEGs) and enriched signaling pathways associated with OA. An in vitro OA-like model was established using interleukin-1β (IL-1β)-stimulated SW1353 cells, which were subsequently treated with different concentrations of PA. PA suppressed IL-1β-induced expression of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), matrix metalloproteinase-3 (MMP-3),matrix metalloproteinase-13 (MMP-13), and A Disintegrin And Metalloproteinase with Thrombospondin Motifs 5 (ADAMTS-5) and attenuated apoptosis by modulating Bcl-2-associated X protein (BAX) and B-cell lymphoma 2 (BCL-2) expression. Notably, target prediction and dual-luciferase reporter assays confirmed that mitogen-activated protein kinase kinase 6 (MAP2K6) is a direct target of microRNA-29a-3p (miR-29a-3p), and PA increased miR-29a-3p expression in the in vitro OA-like model. Furthermore, PA inhibited MAP2K6-mediated activation of the p38 mitogen-activated protein kinase (p38 MAPK) pathway, thereby attenuating downstream inflammatory and apoptotic signaling. These findings suggest that PA exerts chondroprotective effects by modulating the miR-29a-3p/MAP2K6/p38 MAPK signaling axis, highlighting its therapeutic potential and supporting the development of miRNA-based therapeutic strategies for OA. - Source: PubMed
Publication date: 2026/09/16
Wang WeiZhang TaoYu ChaoXu Zhi-XiongLuo LongDong LiangXu Wen-Sheng - To investigate the expression level of mitogen-activated protein kinase kinase 6（MAP2K6） in human nasopharyngeal carcinoma（NPC） tissues and its correlation with Epstein-Barr virus（EBV） infection and clinical prognosis. Pathological sections of cancer tissues and paired adjacent non-tumor tissues were collected from 78 newly diagnosed NPC patients admitted to the Third Affiliated Hospital of Kunming Medical University from January 2018 to December 2018. Immunohistochemical staining was performed to detect the relative expression level of MAP2K6 protein. Fresh cancer tissues and adjacent non-tumor tissues wereobtained from 18 patients, and reverse transcription-quantitative polymerase chain reaction（RT-qPCR） was used to measure the relative expression level of MAP2K6 mRNA. The chi-square test was employed to analyze the correlation between MAP2K6 protein expression and clinicopathological characteristics of NPC. The Kaplan-Meier method was used to plot survival curves and evaluate survival time, and the log-rank test was applied to verify the factors related to prognosis. Multivariate Cox regression analysis was conducted to identify independent risk factors affecting the prognosis of NPC. According to the serum EBV DNA copy number, the Spearman test was used to analyze the correlation between the relative expression level of MAP2K6 and EBV DNA copy number. Compared with cancer tissues, the expression level of MAP2K6 mRNA in adjacent non-tumor tissues was significantly increased, and the difference was statistically significant（<0.05）. The positive expression rate of MAP2K6 protein in adjacent non-tumor tissues was 62.8%, which was significantly higher than that in cancer tissues, and the difference was statistically significant（<0.05）. Expression of MAP2K6 was correlated with T stage, N stage, VCA-IgA and EBV DNA copy number（<0.05）. The expression level of MAP2K6, T stage, VCA-IgA and EBV DNA copy number had significant effects on the survival rate of NPC patients（<0.05）. MAP2K6 expression and EBV DNA copy number were independent risk factors affecting the overall survival of NPC patients. When divided into 2 groups or 4 groups according to EBV DNA copy number, the expression level of MAP2K6 protein was negatively correlated with EBV DNA copy number. MAP2K6 is highly expressed in adjacent non-tumor tissues, correlates with the clinicopathological characteristics of patients, is positively correlated with prognosis, and negatively correlated with EBV DNA copy number. It exerts a tumor-suppressive effect in NPC and is expected to be a therapeutic target or biomarker for the prognostic evaluation of NPC. - Source: PubMed
Gao LinWang QingZhang RanLin FushengYang Jie - Boar sperm freezability (SF) is an economically important trait that influences reproductive efficiency and genetic improvement in pigs. However, its genetic basis remains poorly understood. In this study, semen samples from 382 Duroc and 151 Yorkshire boars were evaluated for sperm motility and recovery rate, and all individuals were genotyped using an 80K SNP array. Within-breed GWAS were first conducted, followed by a meta-analysis integrating the GWAS results from both boars. Individuals were classified into GSF and PSF groups based on sperm recovery rate for subsequent selection signature analysis. The results showed that Duroc boars exhibited significantly higher SF than Yorkshire boars. Heritability estimates for SF were moderate, with values of 0.35 in Duroc and 0.30 in Yorkshire. GWAS identified 10 significant SNPs in Duroc and 33 in Yorkshire associated with SF. Meta-analysis further detected 12 significant SNPs, annotated to candidate genes such as CCDC181, PARN, SOX9, and NCKAP5L. Association analysis identified ten representative variants significantly correlated with sperm recovery rate, with variants in PARN and MAP2K6 showing strong additive effects. Selection signature analysis revealed multiple genomic regions under differential selection between GSF and PSF groups, identifying several candidate genes associated with SF. Functional enrichment analysis indicated that these genes are mainly involved in spermatogenesis, flagellar motility, cellular stress response, and cold adaptation pathways. Overall, this study provides novel insights into the genetic architecture of boar SF and identifies potential molecular markers for genetic improvement and functional genomic studies in pigs. - Source: PubMed
Publication date: 2026/07/22
Wu SiwenHe JianLiang QianxiLi XuehuaLu ZhuodaLi ZhiliJi HuiFeng YaoZhuang ZhanweiZhao Yunxiang - PARP inhibitors (PARPi) selectively target cancers with homologous recombination deficiency (HRD), yet emerging evidence suggests that additional determinants beyond BRCA/HRD status modulate PARPi response. Here, by integrating unbiased proteomic profiling with systematic drug-response phenotyping across 11 ovarian cancer cell lines, we identify MAP2K6 as a previously unrecognized regulator of PARP1 activation and an important determinant of PARP inhibitor sensitivity in BRCA-wild-type ovarian cancer. MAP2K6 promotes global PARP1-mediated PARylation and enhances cellular responsiveness to the PARP inhibitor olaparib both in vitro and in vivo. Mechanistically, MAP2K6 can phosphorylate and activate HMGA1, which in turn transcriptionally upregulates NAMPT, resulting in increased intracellular NAD levels, and sustained PARP1 activation. Genetic depletion of HMGA1 or NAMPT abolishes MAP2K6-driven PARylation and PARPi sensitization, whereas loss of MAP2K6 reduces NAD levels, attenuates PARP1 activation, and confers resistance to PARPi. We propose that MAP2K6 promotes PARP inhibitor sensitivity by coupling metabolic control of NAD supply to PARP1 activation, thereby potentially helping extend the therapeutic scope of PARP inhibition beyond homologous recombination-deficient ovarian cancer. - Source: PubMed
Publication date: 2026/07/17
Wen MingChen ZhuohangZhan DongdongLiu MingweiTan ShuranZhu FangYang SiyuXie JinruZhang YuLi JianjianSun LunquanTan Rong - Psoriasis is a chronic inflammatory autoimmune skin disease for which no standardised and reliable molecular biomarkers of disease course or activity are currently available. Here, we aimed to identify serum biomarkers of psoriasis. Serum samples from 40 patients with psoriasis and 40 healthy volunteers were analysed using ELISA and Proximity Extension Assay proteomics. ELISA revealed significantly increased serum levels of AGO2 and APOA1 in psoriatic patients versus controls, with a strong association between APOA1 and psoriasis (OR = 20.72, 95% CI of 4.57-93.87, = 0.000137). Targeted serum proteomics additionally identified 35 differentially expressed proteins, including well-known psoriasis drivers (e.g., top upregulated IL17A and SERPINB4). The most downregulated was adrenomedullin (ADM, FC = -10.12). For 14 altered proteins, no previous direct associations with psoriasis were reported. Among them, DEFB103A_DEFB103B and DSG3 showed the best discrimination between psoriasis and control samples, while SERPINB4 correlated with psoriasis severity. APOA1, DEFB103A_DEFB103B, and DSG3 emerge as novel candidate circulating psoriasis biomarkers, and SERPINB4 as a biomarker of psoriasis severity. The functional role of DSG3 and other newly identified proteins (ACRV1, HAO1, ADH4, GPD1, GFER, PTGES2, DSG3, AFAP1L1, GALNT3, RASGRP2, MAP2K6, LXN, NBEAL2, and VPS54) in psoriasis requires further studies. - Source: PubMed
Publication date: 2026/06/26
Dźwigała MonikaSys DorotaŻycka-Krzesińska JoannaRybicka BeataPopławski PiotrWalecka-Herniczek IrenaPiekiełko-Witkowska AgnieszkaBogusławska Joanna