b4GALNT2 ELISA kit
- Known as:
- b4GALNT2 Enzyme-linked immunosorbent assay test reagent
- Catalog number:
- DL-b4GALNT2-Hu
- Product Quantity:
- 96T
- Category:
- Elisa Kits
- Supplier:
- WDSTD
- Gene target:
- b4GALNT2 ELISA kit
Related genes to: b4GALNT2 ELISA kit
- Gene:
- B4GALNT2 NIH gene
- Name:
- beta-1,4-N-acetyl-galactosaminyltransferase 2
- Previous symbol:
- GALGT2
- Synonyms:
- Sda, Cad
- Chromosome:
- 17q21.32
- Locus Type:
- gene with protein product
- Date approved:
- 2004-02-20
- Date modifiied:
- 2016-10-05
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- To address critical challenges in clinical blood shortages and limited preservation periods, particularly affecting emergency transfusions, transgenic pig red blood cells (RBCs) emerge as a promising solution. We performed multi-gene editing on pig RBCs by knocking out three Xeno antigen genes () and introducing high expression of human and , generating 5-gene edited (5GE) RBCs. After co-incubating with human blood, we assessed their survival rate, along with physicochemical indicators related to hemolytic reactions and inflammatory responses. We further compared the physicochemical properties between transgenic pig blood and human blood. 5GE RBCs showed no significant agglutination, only mild hemolysis, and markedly reduced cytotoxicity. Binding by human antibodies and complement was significantly diminished. This study demonstrates the strong potential of genetically engineered porcine RBCs for emergency human transfusion, offering a viable alternative to alleviate blood shortages, especially when human RBCs are unavailable. - Source: PubMed
Publication date: 2026/07/14
Lu PanWang YongDu JiaxiangYan JieDeng ShaopingHe DongshengRen ZhipengDai ZiqiangLi DianyuanPan Dengke - The CRISPR/Cas9 system has been widely used for gene editing in various species; however, mosaicism remains a significant challenge. This study aimed to improve gene editing efficiency and reduce mosaicism in porcine embryos by exploring double electroporation pre- and post-in vitro fertilization combined with zona pellucida (ZP) removal. We evaluated the effects of these treatments on the development and mutation rates of oocytes/zygotes edited with guide RNAs (gRNAs) targeting , , or genes. Double electroporation significantly increased the total and biallelic mutation rates in ZP-intact zygotes but not in ZP-free zygotes edited using -targeted gRNAs. All blastocysts from ZP-free zygotes exhibited biallelic mutations following double electroporation. For the gene, all blastocysts exhibited mutations (biallelic mutations ≥ 80%); however, double electroporation and ZP removal did not affect their mutation rates or efficiency. For the gene, double electroporation significantly increased total mutation rates in ZP-intact zygotes, whereas all blastocysts from ZP-free zygotes showed biallelic mutation. These findings suggest that double electroporation, particularly with ZP removal, may enhance gene-editing efficiency, reduce mosaicism and improve the success of genetic modifications. - Source: PubMed
Publication date: 2026/06/20
Torigoe NanakaOtoi TakeshigeWittayarat ManitaWidodo Oky SetyoTharasanit TheerawatChatdarong KaywaleeNagahara MegumiHirata MakiTanihara FuminoriNamula Zhao - Swine Leukocyte Antigen-DR knockout (SLA-DR KO) pigs were created and evaluated for safety/infectious profile and the ability to function in a preclinical model of xenotransplantation. - Source: PubMed
Publication date: 2026/06/05
Adams Andrew BTector MattBurlak ChristopherEstrada JoseReyes LuzCopsel SabrinaMuniz ClaudiaNovara Gennuso VictorMartucci MelanieIwakoshi NealDryden MichaelFaber DavidRay BryanHaver HollyHariharan JayasreeVianna RodrigoTector Alfred J - Litter size is an economically important trait in sheep and is influenced by ovarian follicular development and granulosa cell (GC) function. This study investigated the association between B4GALNT2 polymorphisms and litter size and examined whether B4GALNT2 is regulated by miR-133 in ovine GCs. A total of 384 two-year-old multiparous ewes, including 231 Gobi short tail (GB) sheep and 153 Ujimqin (UN) sheep, were used for variant detection, MassARRAY genotyping, and association analysis. Meanwhile, cultured ovine GCs were used to evaluate the effects of B4GALNT2 overexpression and knockdown on cell proliferation and apoptosis. In addition, candidate microRNAs targeting litter size-associated variants in the 3' untranslated region were predicted and validated using dual-luciferase reporter assays, quantitative real-time PCR, Western blotting, EdU staining, and flow cytometry. The results showed that 19 single nucleotide polymorphisms were identified in B4GALNT2, including 17 novel and two known variants. Among them, four variants in GB sheep and three variants in UM sheep were significantly associated with litter size. Furthermore, B4GALNT2 overexpression promoted GC proliferation and inhibited apoptosis, whereas B4GALNT2 knockdown produced the opposite effects. Moreover, the g.37121000C > T variant was confirmed as an allele-specific miR-133 target site. miR-133 overexpression reduced B4GALNT2 expression, suppressed GC proliferation, and promoted apoptosis, whereas miR-133 inhibition reversed these effects. Thus, these results indicated that B4GALNT2 is associated with litter size and could support GC proliferation, whereas miR-133 negatively regulates B4GALNT2 through the g.37121000C > T site. - Source: PubMed
Publication date: 2026/05/12
Bai YanyuYue YongliAn XinyueXu JianyuZhang MinAlatan SuheCao GuifangCang MingTong Bin - The intestinal microbiota is integral to host health, metabolism, and colonization resistance. Antibiotics can disrupt microbial homeostasis, leading to dysbiosis and altered colonization resistance. While antibiotic-induced microbiota disruption is well-documented, less is known about how host genetics shapes post-antibiotic recovery. Here, we investigate the impact of , a blood-group-related glycosyltransferase gene, on microbiota recovery following antibiotic treatment. Using a longitudinal, multi-omic approach-including 16S rRNA gene sequencing, metagenomics, and metatranscriptomics-we compare the microbiota dynamics of and mice after treatment with streptomycin, kanamycin, and vancomycin. Our findings reveal that mice exhibit faster recovery of microbial diversity and composition following streptomycin treatment compared to their counterparts. This accelerated recovery is associated with higher relative abundance of taxa such as , and other Lachnospiraceae, and increased expression of motility-related genes, and differential regulation of antibiotic resistance genes (ARGs), including the aminoglycoside nucleotidyltransferase genes and . Genotype-dependent differences in recovery were most pronounced following streptomycin and were not consistently observed with kanamycin or vancomycin, indicating an antibiotic-by-genotype interaction shaped by the -associated microbiota. These results underscore the role of host genetics in shaping microbiota response and recovery following antibiotic exposure. By demonstrating the interplay between glycosylation-mediated microbiota composition, antibiotic response, and microbial recovery, our study may provide insights into the potential for personalized approaches to mitigate dysbiosis-related health outcomes. - Source: PubMed
Publication date: 2026/04/30
Čepić AleksaRausch PhilippGeese TheresaDempfle AstridGrassl Guntram ABaines John F