CBX3 ELISA kit
- Known as:
- CBX3 Enzyme-linked immunosorbent assay test reagent
- Catalog number:
- DL-CBX3-Hu
- Product Quantity:
- 96T
- Category:
- Elisa Kits
- Supplier:
- WDSTD
- Gene target:
- CBX3 ELISA kit
Related genes to: CBX3 ELISA kit
- Gene:
- CBX3 NIH gene
- Name:
- chromobox 3
- Previous symbol:
- -
- Synonyms:
- HP1Hs-gamma
- Chromosome:
- 7p15.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-10-12
- Date modifiied:
- 2015-11-24
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- Raman spectroscopy and mass spectrometry-based proteomics offer deeply complementary yet largely disconnected views of cancer biology: the former provides a label-free, real-time biochemical phenotype, while the latter delivers a quantitative inventory of specific protein effectors. Bridging this gap remains a fundamental challenge in analytical biomedicine. Here, we introduce Spectral-Proteomic Integration Analysis (SPIA)─a novel, data-driven integrative framework that systematically links Raman spectroscopic phenotypes with quantitative proteomic profiles through machine learning and statistical correlation. Using a DMBA-induced rat breast cancer model with and without Toremifene (TOR) intervention, SPIA dynamically maps tumor microenvironment remodeling, capturing progressive collagen deposition and lipid metabolic reprogramming. An SVM classifier trained on Raman spectra achieves exceptional diagnostic accuracy (AUC ≥ 99.0%) and successfully predicts TOR therapeutic response. Proteomic analysis identifies 1,350 differentially expressed proteins, with convergent machine learning feature selection (LASSO, Random Forest, XGBoost) pinpointing core regulators including Luc7l2, Nucb1, Cbx3, and Csnk2a1. Crucially, Spearman correlation analysis between key Raman bands and core DEPs reveals strong, statistically robust associations (median ρ ∼ 0.75 in the 1533-1669 cm-1 region), empirically validating SPIA's core integrative logic. Leveraging this multimodal map, we elucidate a multitarget mechanism for TOR involving concurrent suppression of collagen deposition and correction of aberrant lipid metabolism. SPIA establishes a powerful, generalizable paradigm for integrating phenotypic and molecular data, with broad implications for biomarker discovery, drug mechanism elucidation, and precision oncology. - Source: PubMed
Sun ShanchuanQiao JianpingZhang HuadongLi YongqiZhang HaoranZhang JiayongGao MengmeiLiu Dong-XuSun JiandeYu ZhigangZheng Chao - tRNA-derived small RNAs (tsRNAs) are noncoding RNAs generated from precursor or mature tRNAs. Basal tsRNA levels are detectable under physiological conditions, but their production increases sharply under cellular stress. tsRNAs regulate gene expression through miRNA-like targeting, translational control, and intercellular communication. In vascular remodelling disorders-including atherosclerosis (AS), aortic dissection (AD), and in-stent restenosis (ISR)-vascular smooth muscle cells (VSMCs) undergo phenotypic switching from contractile to synthetic states. Pulmonary hypertension (PH) shares similar phenotypic features, but the current evidence on the role of tsRNAs in PH remains largely predictive. Several experimentally studied tsRNAs affect VSMC phenotypic behaviour in disease- and context-dependent manners by targeting key signalling molecules, including STAT4, FMOD, FAS, and CBX3/HP1γ, with downstream consequences for contractile gene expression and proliferative behaviour. Notably, these tsRNAs act bidirectionally: 5'-tiRNA-Cys-GCA and tRF-Glu-CTC (via FMOD targeting) suppress the synthetic phenotype and neointimal formation, whereas tRF-Gln-CTG promotes it. We propose an evidence-mapped conceptual framework organized by regulatory layers-transcriptional, posttranscriptional, and chromatin-associated regulation-while acknowledging that cross-layer interactions remain to be experimentally verified. Clinically, tsRNAs hold promise as liquid biopsy biomarkers and therapeutic targets, but the current evidence is preliminary and requires validation against established biomarkers before translational application. This review synthesizes the current evidence on tsRNA biology in the context of VSMC phenotypic switching and outlines a roadmap for future research. - Source: PubMed
Publication date: 2026/08/27
Li KunLi Xinqiu - Heterochromatin Protein 1γ, encoded by the gene, is a crucial epigenetic regulator that plays an essential role in mammalian meiotic progression. However, the functional divergence and conservation of this protein in teleosts-organisms possessing duplicated Cbx3 paralogs due to whole-genome duplication-remain to be elucidated. Building on previous research, we focused on in Nile tilapia (), a significant aquaculture species and an excellent model for teleost reproductive studies, emphasizing its role in spermatogenesis. Expression analysis revealed that Cbx3a is localized to primordial germ cells and is sustained in spermatogonia, spermatocytes, and spermatids during spermatogenesis. CRISPR/Cas9-mediated knockout of demonstrated that Cbx3a deficiency induces germ cell apoptosis, meiotic arrest, and sperm defects, including shortened tails and impaired motility, resulting in profound defects in sperm quantity and quality, strongly implying compromised male fertility. Transcriptomic analysis further identified dysregulated molecular pathways, including cytokine signaling and neuroactive ligand-receptor interactions. This provides novel mechanistic insights into HP1γ-mediated epigenetic regulation of meiosis. Notably, mutants exhibited phenotypic bifurcation: a subset showed meiotic defects accompanied by sporadic germ cell apoptosis, whereas others underwent full meiotic arrest with pervasive germ cell apoptosis in adult gonads. Collectively, these findings clarify the essential and conserved role of Cbx3a/HP1γ in Nile tilapia spermatogenesis, thereby advancing the field of vertebrate reproductive epigenetics and providing a valuable theoretical basis for potential applications in reproductive management, such as improving sperm quality. - Source: PubMed
Publication date: 2026/07/31
Jian HongqinWu JiahongFeng RuijuanZhang LiangZhou LiLiu Xingyong - In response to stress, cells exploit plasticity to self-activate and form protrusions that explore the environment and guide migration. While protrusion initiation is well characterized, their maturation and functional composition remain poorly understood. Here, using a new pooled genetic approach (ReGenT-seq) to interrogate chromatin factors controlling epidermal progenitor activation, we unexpectedly identified Cbx3 as a critical determinant of protrusion formation. Beyond its nuclear activities, we revealed a cytoplasmic moonlighting function of CBX3 that governs the subcellular localization of specific mRNAs within polarizing protrusions of migrating epidermal progenitor cells, as well as in invadopodia of squamous carcinoma cells. CBX3-dependent mRNA transport promotes the localized establishment of multiple organelles, including the endoplasmic reticulum and lysosomes, within maturing protrusions, and regulates focal adhesion dynamics. Together, our findings uncover a multilayered gene regulatory mechanism controlling cell motility through protrusionogenesis and identify a chromatin-to-cell-protrusion mRNA transport pathway that represents a potential therapeutic target for enhancing wound healing and limiting cancer invasion. - Source: PubMed
Publication date: 2026/06/27
Duval CarlottaLauria AndreaCroce AlessandroLevra Levron ChiaraAnsai OsamuCaizzi LiviaAnselmi FrancescaPiacenti GabrieleBalma ElisaElettrico LucaAlbano AlessiaDonna DanielaNeri FrancescoBorella FulvioWatanabe MikaNatsuga KenProserpio ValentinaOliviero SalvatoreDonati Giacomo - Immune thrombocytopenia (ITP) is the most common acquired bleeding disorder where considerable dysfunction of regulatory T cells (Tregs) contributes to the loss of immune tolerance. CD38 is a transmembrane glycoprotein equipped with ectoenzymatic activities. It affects other NAD-consuming enzymes such as deacetylase Sirtuin-1 (Sirt-1), which is pivotal for Treg stability. Anti-CD38 monoclonal antibodies exert ectoenzyme-modulating capacity and has been proven to rapidly boost and maintain platelet levels in patients with ITP. - Source: PubMed
Publication date: 2026/06/26
Zhu MingfangWang HaoyiXu YitongLi YubinWang WanruWang LingjunWang RuixueZhang PingWang RutingCao JunyingWang LinShi YanHou MingHou Yu