AQP3 ELISA kit
- Known as:
- AQP3 Enzyme-linked immunosorbent assay test reagent
- Catalog number:
- DL-AQP3-Hu
- Product Quantity:
- 96T
- Category:
- Elisa Kits
- Supplier:
- WDSTD
- Gene target:
- AQP3 ELISA kit
Related genes to: AQP3 ELISA kit
- Gene:
- AQP3 NIH gene
- Name:
- aquaporin 3 (Gill blood group)
- Previous symbol:
- -
- Synonyms:
- GIL
- Chromosome:
- 9p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1994-07-25
- Date modifiied:
- 2019-04-23
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- Injectable sodium hyaluronate (NaHA) is extensively utilized in aesthetic medicine as a dermal hydrating agent. However, there are few standardized, human-relevant preclinical methods that can reliably evaluate the moisturizing performance of those products. Existing animal and simplified cell-based models show restricted physiological relevance and insufficient sensitivity. To address this gap, we established a depot-mimicking reconstructed human full-thickness skin (RhFS) platform incorporating an inclusion-based intradermal delivery strategy. Instead of conventional mixing, this strategy mimics the clinical 'depot effect' of intradermal injection, allowing for the simultaneous assessment of cellular responses and tissue-level hydration dynamics. It forms a localized NaHA depot within the dermal compartment of RhFS and preserves spatial hydration gradients, which are lost when NaHA is mixed homogenously. The platform integrates physicochemical characterization of polymer-bound water states with cellular and tissue-level functional readouts. By quantifying key biomarkers, including CD44, aquaporin-3 (AQP3) and natural moisturizing factors (NMFs), our results demonstrate that the inclusion-based delivery strategy significantly outperforms conventional mixing approaches in activating epidermal hydration pathways. Crucially, this platform effectively distinguished the moisturizing efficacy of multiple commercial NaHA formulas, thereby revealing a structure-activity relationship between water-binding states and biological outcomes. Overall, this study presents a reproducible, mechanism-informed and human-relevant framework for preclinical performance evaluation of NaHA-based injectable biomaterials and provides a sensitive alternative to conventional animal-based approaches. - Source: PubMed
Publication date: 2026/02/09
Shi JianfengXu WennaLiu HongfuShan FengjuanWang RuiKe LinnanHan QianqianLu Yong - This study examines whether differences in genetic selection pressure between breeds are reflected in sperm cryoresistance and AQP3 abundance and dynamics in membrane domains. Semen from nine bulls (five Holstein and four Pirenaica, a minimally selected native breed) was analyzed under chilled and frozen-thawed conditions using 36 ejaculates (four per bull), each evaluated in both conditions. Sperm motility was analyzed using a CASA system, sperm viability was assessed by fluorescence with the SQS2 Seminal Quality System, and membrane and acrosomal integrity were analyzed by fluorescence microscopy. Spermatozoa were examined for the presence, abundance and distribution of AQP3 by Western blot and immunocytofluorescence (ICF). In chilled semen, Holstein exhibited a higher sperm viability, and membrane and acrosome integrity (p < 0.05). In frozen-thawed semen, Holstein bulls exhibited superior progressive motility (p < 0.05) and a higher proportion of sperm with intact plasma membranes and acrosomes (p = 0.05). The cryoresistance index was higher in Holstein samples for total motile sperm (p < 0.05). No significant differences in isocitrate dehydrogenase (IDH) activity were observed between breeds under either condition. ICF revealed that AQP3 was localized in the midpiece and principal piece. The freeze-thaw process led to a significant increase (p < 0.05) in the proportion of spermatozoa exhibiting AQP3 in the midpiece and principal piece of Holstein samples. Holstein bulls exhibited significantly higher AQP3 abundance than Pirenaica in frozen-thawed semen (p < 0.05). A similar tendency was observed in chilled samples, where Holstein semen showed higher AQP3 levels than Pirenaica (p = 0.09). The results suggest that higher sperm cryoresistance in Holstein bulls was associated with higher AQP3 abundance and capacity for AQP3 redistribution along the flagellum during cryopreservation. - Source: PubMed
Publication date: 2026/04/28
Alba EstherCastaño CristinaVelázquez RosarioToledano-Díaz AdolfoSantiago-Moreno Julián - The Tuihong Formula (THF) has been used in traditional Chinese folk medicine as a topical herbal preparation for the treatment of cutaneous disorders, and its therapeutic efficacy is well established. Although THF has shown promising clinical efficacy in atopic dermatitis (AD), but its pharmacological mechanisms remain unclear. - Source: PubMed
Publication date: 2026/04/25
Huang YingqiLin WeijunChen XiaoyanLin FuyuanLi JiajieLiu YaoshengLiu YumeiLuo GuangpuDu XianhuaDuan LixinZhan YaxianZhao Tie - The aquaporin genes (aqps) are widely distributed among vertebrates. In cartilaginous fish, aqp3 and aqp10 are duplicated, whereas many species have lost aqp8. While the solute permeability of aquaglyceroporins and Aqp8 exhibits significant diversity, systematic evaluations of their activity within species are limited. Consequently, we conducted a comprehensive analysis of the aqps in various cartilaginous fish genome databases. Our analysis revealed that the sharpnose sevengill shark (Heptranchias perlo) and North Pacific spiny dogfish (Squalus suckleyi) possess all aqp genes found in cartilaginous fish, rendering them suitable for comparative analysis of Aqp activity. The activity of all aquaglyceroporins and Aqp8 in the sharpnose sevengill shark was analyzed using Xenopus oocyte swelling assays. The water permeability of Aqp3c2, Aqp8, Aqp9, Aqp10c1, and Aqp10c2; the glycerol permeability of Aqp3c2, Aqp9, and Aqp10c2; the urea permeability of Aqp3c2, Aqp8, Aqp9, and Aqp10c2; and the boric acid permeability of Aqp8 and Aqp9 were determined. The urea and boric acid permeabilities of Aqp9 were higher than those of other Aqps. Our results provide genomic evidence for the loss of aqp7 and further elucidate the diversification of aqp8 in cartilaginous fishes. Moreover, the urea permeability of Aqp9 suggests a functional role in their unique urea-based osmoregulation strategy. - Source: PubMed
Hidaka ShinichiroNagashima AyumiKato Akira - The global prevalence of functional constipation has witnessed a marked increase due to the imbalanced dietary pattern. The prebiotic function in vitro and the anti-constipation efficacy of garlic polysaccharides (GP) in loperamide-induced constipated mice were evaluated. During in vitro simulated digestion and fecal fermentation, GP showed digestive resistance properties and enhanced abundance of the probiotic bacteria and . In vivo, GP significantly attenuated intestinal oxidative stress. Moreover, GP treatment dose-dependently ameliorated constipation parameters. Notably, GP restored colonic barrier integrity: GP potently upregulated the mRNA expression of tight junction proteins ZO-1 and occludin in the mechanical barrier and exhibited the most substantial restoration of both MUC2 and MUC4 expression levels in the mucins barrier, as well as reduced pro-inflammatory cytokines of TNF-α, IL-1β, IL-17 and elevated anti-inflammatory IL-10 in the immune barrier. Furthermore, GP regulated VIP-cAMP-PKA-AQP3 signaling pathway, which subsequently promoted intestinal water transport and mucus secretion. GP significantly regulated the gut microbiota-SCFAs axis by lowering the ratio and suppressing the level of pathobiont . GP intervention significantly elevated total SCFAs compared to the MOD group ( < 0.05). These results elucidated GP's multi-target mechanism against functional constipation via antioxidant, barrier-repairing, VIP pathway-regulating, and microbiota-directed SCFAs axis. - Source: PubMed
Publication date: 2026/03/17
Li JingfangMiao JiaxinLi TianyiXie ChanyuanXu WentaoChang ShiminChai Ran