Bcl2 ELISA kit
- Known as:
- Bcl2 Enzyme-linked immunosorbent assay test reagent
- Catalog number:
- DL-Bcl2-Hu
- Product Quantity:
- 96T
- Category:
- Elisa Kits
- Supplier:
- WDSTD
- Gene target:
- Bcl2 ELISA kit
Related genes to: Bcl2 ELISA kit
- Gene:
- BCL2 NIH gene
- Name:
- BCL2 apoptosis regulator
- Previous symbol:
- -
- Synonyms:
- Bcl-2, PPP1R50
- Chromosome:
- 18q21.33
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2019-04-23
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- Adoptive cellular immunotherapy (ACT) such as CAR‑T therapy holds promise for cancer treatment. However, genetically engineered T cells often undergo terminal differentiation during ex vivo expansion, which limits their persistence and antitumor efficacy . Early‑differentiated T‑cell subsets exhibit better survival and proliferative capacity after infusion. In our previous work, we isolated four T‑cell subsets at different differentiation stages: naïve T cells (T), stem cell‑like memory T cells (TSCM), central memory T cells (T), and effector memory T cells (T), and obtained their miRNA expression profiles via high‑throughput sequencing. In the present study, we found that hsa‑miR‑142‑5p is highly expressed in TSCM cells and gradually decreases during T‑cell differentiation. - Source: PubMed
Publication date: 2026/05/18
Wang HongqiongXia ShengfangChen JiaZhong HuishanZeng XianpeiLu ZitaoZhang WenfengWu Fenglin - Myelodysplastic syndromes (MDS) represent clonal hematopoietic stem cell disorders with inherent propensity for leukemic transformation. Emerging evidence implicates dysregulated Endoplasmic-Reticulum stress (ER-stress) in sustaining malignant clones through adaptive suppression of pro-apoptotic PERK/CHOP signaling. Celastrol serves as a natural compound with documented ER-stress modulating activity in solid tumors. However, the beneficial effect and molecular mechanisms of celastrol on MDS are unknown. - Source: PubMed
Publication date: 2026/06/03
Zheng FuxiangShao WenjunLiu LiYang ChaoyingHu DingyuSu XiaolingLi XuanCao PengfeiLiu JingXu YunlongLiang LongHe JunZhang Ji - Maternal preeclampsia (PE) contributes to neurodevelopmental disorders. However, the underlying molecular mechanism remains unclear. We investigated the potential biological regulatory mechanisms by establishing an animal model and primary cell culture. Data from the Adolescent Brain Cognitive Development human study revealed that the striatum mediates maternal PE and offspring behavioral disorders. In a rat PE model, offspring exhibited abnormal behaviors and reduced striatal vascular density, with disrupted blood-brain barrier (BBB) and swollen astrocytes. Primary astrocytes and brain microvascular endothelial cells (BMECs) were cultured. Astrocytes from PE-exposed offspring weakened tube-formation ability and BBB-related protein expression in BMECs. Transcript sequencing identified B-cell lymphoma 2 (BCL2) as a key neurodevelopmental gene affected by PE. Astrocytes with BCL2 knockdown weakened tube-formation and BBB-related protein expression in BMECs, matching PE offspring astrocytes. BCL2 knockdown in newborn rats resulted in disrupted BBB, and abnormal behaviors, similar to PE-exposed offspring. In summary, reduced astrocyte BCL2 levels contributed to BBB disruption in the striata of PE-exposed offspring, providing new insights into the mechanisms underlying neurodevelopmental disorders. - Source: PubMed
Publication date: 2026/06/02
Zhang JiashuoZhang MengtingZhao ZhengyangXie MinWei MenghanLiu SiyiWu YuluTao ShiwanLv QiuyueMa XiaoyingHuang YunqiLi MingliZou MinWang Qiang - In this study, a multifunctional nanocomposite based on zeolitic imidazolate framework-8 was engineered for the enhanced delivery of resveratrol to colorectal carcinoma cells. The system comprised resveratrol-encapsulated ZIF-8 nanoparticles sequentially modified with polydopamine, hyaluronic acid, and folic acid (ZIF-8-Res-PDA-HA-FA). Physicochemical characterization revealed a Z-average hydrodynamic diameter of 194 nm, a polydispersity index of 0.197, and a zeta potential of - 40.55 mV, indicative of a narrow size distribution and robust colloidal stability. Drug encapsulation efficiency achieved 81.9%, with in vitro release kinetics demonstrating pH-responsive behavior characterized by 58.7% cumulative release reached 59.9% at pH 5.5 and 33.2% at pH 7.4 after 72 h, and further increased to 81.4% at pH 5.5 by 120 h, confirming acid-triggered drug liberation. Molecular docking simulations of resveratrol against the Bcl-2 BH3 groove yielded a modest binding affinity (S-score = - 6.23 kcal/mol) with a single validated hydrogen bond to Ala4 (2.83 Å); however, this computational prediction does not constitute evidence of cellular target engagement, and functional assays were not performed. In HCT116 cells, the nanocomposite exhibited concentration-dependent cytotoxicity with a calculated IC₅₀ of 54.4 µg/mL following 24-h exposure, while human dermal fibroblasts retained > 70% viability at concentrations up to 500 µg/mL, suggesting preferential antiproliferative activity against malignant cells. Flow cytometric analysis confirmed apoptotic induction, with treatment reducing cellular viability from 97.4% to 58.7% and increasing late-stage apoptosis to 28.4% at 74 µg/mL. Quantitative PCR demonstrated transcriptional upregulation of p53 (9.96-fold) and p21 (2.3-fold) at 48 h, whereas caspase-8 expression remained at baseline levels, implicating the intrinsic apoptotic pathway as the predominant mechanism. Wound-healing assays at sub-cytotoxic concentrations revealed significant inhibition of cellular motility (25-30% closure versus 85-90% in controls; p < 0.001). Receptor-mediated cellular uptake was not experimentally verified in this study; therefore, the observed cellular effects cannot be attributed to active targeting. While these findings demonstrate in vitro therapeutic potential, several critical limitations must be addressed: receptor-mediated cellular recognition was not experimentally verified, protein-level confirmation of gene expression changes is absent, and in vivo pharmacokinetics remain to be established. - Source: PubMed
Publication date: 2026/06/02
Seyedi SabaHomayouni Tabrizi MasoudNeamati AliMasoumian Hosseini Mohsen - The biological synthesis of nanoparticles (NPs) has attracted because of their non-toxic abilities. We reported the synthesis of ZnO nanoparticles (ZnO NPs) via an extract of the Amycolatopsis roodepoortensis strain EA7. Their physical characteristics were investigated with XRD, FT-IR, SEM, TEM, DLS, EDX, and zeta potential analyses. The toxicity of nanoparticles (NPs) was investigated via antimicrobial and the MTT assays in the HT-29, HEK293, and HDF1BOM cell lines.The expression levels of genes related to apoptosis (ATM, ATR, CHK1, and CHK2), anti apoptose (MMP-9 and Bcl-2), cell death, and cell cycle distribution were determined in the HT-29 cell line. ZnO nanoparticles (ZnO NPs) exhibited stronger antibacterial efficacy against clinical and standard Staphylococcus aureus (250 and 125 μg/mL) than Pseudomonas aeruginosa (500 μg/mL) by the MIC assay. The anticancer effects of these nanoparticles (NPs) demonstrated a dose‒dependent relationship against HT-29, HEK-293, and HDF1BOM cell lines. The IC values were determined to be 47 , 32.88, and 81.37 μg/mL for the HT-29, HEK293, and HDF1BOM cell lines, respectively. The levels of genes related to apoptosis (ATM = 2.35 ± 0.293, ATR = 2.87 ± 0.280, CHK1 = 3.67 ± 0.378, and CHK2 = 5.86 ± 0.495) were significantly increased compared to the control (P ≤ 0.0001). However, no significant decrease was observed in the expression of genes associated with cancer development. Flow-cytometric analysis revealed apoptosis induction (P ≤ 0.001) and cell cycle arrest in G0/G1 phase in the HT-29 cells. Given the results presented, this research could be an important step towards further investigations and the development of novel antimicrobial and anticancer therapeutics. - Source: PubMed
Publication date: 2026/06/03
Amiri ElhamMirpour MirsasanIssazadeh KhosroRasti Behnam