CASP7 ELISA kit
- Known as:
- CASP7 Enzyme-linked immunosorbent assay test reagent
- Catalog number:
- DL-CASP7-Hu
- Product Quantity:
- 96T
- Category:
- Elisa Kits
- Supplier:
- WDSTD
- Gene target:
- CASP7 ELISA kit
Related genes to: CASP7 ELISA kit
- Gene:
- CASP7 NIH gene
- Name:
- caspase 7
- Previous symbol:
- -
- Synonyms:
- MCH3, CMH-1, ICE-LAP3
- Chromosome:
- 10q25.3
- Locus Type:
- gene with protein product
- Date approved:
- 1996-07-22
- Date modifiied:
- 2016-10-05
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- Rosmarinic acid (RA) exhibits anti-oxidant, anti-aging, and anti-inflammatory properties. This study aimed to investigate the effects of RA on amyloid beta (Aβ), phosphorylated tau (p-tau), α-synuclein (α-syn), Abelson tyrosine kinase (Abl), and apoptotic markers in an Alzheimer's disease (AD) model. - Source: PubMed
Şen AysuEroğlu Güneş CananDursun Ebru NurKurar ErcanKutlu Selim - Chiliadenus montanus (Vahl.) Brullo (Asteraceae) is a traditional medicinal plant with reported anti-inflammatory properties; however, its potential anticancer activity and underlying molecular basis remain insufficiently characterized. In this study, a bioactivity-guided integrative approach combining metabolomics, biological evaluation, network pharmacology, and computational modeling was applied to explore the antiproliferative potential of the acetone extract of C. montanus (CME). CME exhibited cytotoxic activity against a panel of 14 human cancer cell lines (IC₅₀ = 1.41-24.78 µg/mL), with HepG2, Panc-1, and HCT-116 cells showing the highest sensitivity relative to a normal fibroblast control (selectivity index > 13). Flow cytometry analysis revealed substantial apoptosis induction and cell-cycle perturbation at the G₀/G₁ and S phases. Consistently, protein expression analysis showed upregulation of p53, Casp7, and BAX together with downregulation of BCL-2, resulting in an increased BAX/BCL-2 ratio across the responsive cell lines, supporting activation of mitochondrial apoptotic signaling. UPLC-TOF-MS/MS profiling tentatively identified 162 metabolites, predominantly flavonoids and organic acids, including luteolin, naringenin, kaempferol derivatives, and γ-linolenic acid. Network pharmacology identified 87 overlapping targets associated with liver cancer-related pathways, highlighting COX-1 (PTGS1), 5-LOX (ALOX5), and oxidative-stress-related proteins as central nodes. Consistent with these predictions, CME inhibited COX-1 and 5-LOX activity (in vitro) with IC₅₀ values of 7.25 µg/mL and 3.21 µg/mL, respectively, and displayed antioxidant activity in DPPH, ABTS, and FRAP assays. Moreover, CME reduced cellular levels of PGE₂, IL-6, and TNF-α in HepG2 cells, indicating suppression of inflammatory signaling. Molecular docking further suggested favorable interactions between several identified metabolites and inflammation-related targets. Overall, CME activity is associated with modulation of apoptotic and inflammatory signaling pathways, supported by biochemical, cellular, metabolomic, and computational analyses. While these findings provide mechanistic insights into the biological activity of C. montanus, further studies, including compound isolation and in vivo validation, are required to confirm these effects and their therapeutic relevance. - Source: PubMed
Publication date: 2026/08/22
Bamagous Ghazi AAlmasmoum Hussain AliGhaith Mazen MAlhakami Rayan FawazMukhtar Mohammed HasanEissa Mohamed EAl-Hazani Tahani M IAlshawwa Samar ZuhairAlrashidi Amal AbdullahNahas Hebatallah H AboSaied Essa M - Caspase-3 (CASP3) and caspase-7 (CASP7) are the two major executioner caspases that are proteolytically activated by upstream initiator caspases. They possess almost indistinguishable activity, which has led to the overall view that these caspases have functionally redundant roles. Here, we generate knock-in mice expressing cleavage-resistant CASP3(D175A) or CASP7(D198A). Our results show that proteolytic activation of CASP3 and CASP7 is decisive for their activity in vivo and controls redundant processes during embryonic development as combined expression of both CASP3(D175A) and CASP7(D198A) causes embryonic lethality. In adult mice, however, activation of CASP3 and CASP7 controls different processes in different tissues, without the involvement of apoptosis. While CASP7 activation is required for male fertility by controlling spermatogenesis, CASP3 activation appears crucial for lymphoid tissue development by regulating interferon signalling. Our findings shed light on emerging roles of caspases in non-apoptotic processes and provide impetus for reconsidering their involvement in physiological and pathological conditions. - Source: PubMed
Publication date: 2026/07/21
Sieg NoëlleStachelscheid JohannaThaqi BesartaSchwab TanjaSchorn FabianHahn DanielaSimon Adrian GeorgTolkach YuriAbedpour NimaTröder Simon EVoigt IngoGaedke FelixWiegmann KatjaBrodesser SusanneSchauss AstridZevnik BrankoKrönke MartinBüttner ReinhardQuaas AlexanderHallek MichaelFritsch MelanieKashkar Hamid - Cataracts are the leading cause of vision loss worldwide. Despite surgery being the only effective treatment, its economic burden highlights the necessity of exploring the pathogenesis of cataracts. In this study, we analyzed 4 large-scale GWAS (genome-wide association study) datasets for cataracts and performed SMR analysis along with heterogeneity in dependent instruments (HEIDI) testing to explore the effects of methylation, expression, and protein QTLs on cataracts. We further validated shared genetic variants through COLOC analysis. Additionally, we searched datasets related to cataracts from the Gene Expression Omnibus (GEO) database for differentially expressed genes (DEGs) and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. By integrating summary-based Mendelian randomization (SMR) results with bioinformatics findings, CASP7 showed a consistent protective-direction association with cataract risk (mQTL: OR [95% CI] = 0.959 [0.941-0.977], FDR-adjusted P = .039; eQTL: OR [95% CI] = 0.897 [0.860-0.937], FDR-adjusted P = .0046; pQTL: OR [95% CI] = 0.597 [0.483-0.738], FDR-adjusted P = .00083). GEO-based analyses provided transcriptomic support for CASP7 involvement in cataract-related lens biology. These findings prioritize CASP7 as a genetically supported candidate protective gene associated with cataract risk. Because this study is based on public summary-level and transcriptomic datasets, the results should be interpreted cautiously and require functional validation in human lens-relevant systems. - Source: PubMed
Yuan ZhengxiDing XiangyuFang XiaolongMu JingqingHua Xia - Semen quality traits in chickens are critical indicators reflecting reproductive efficiency, breeding progress, and economic profitability in the poultry industry. Understanding the genetic architecture and enabling targeted genetic improvement of these traits are essential for enhancing poultry production efficiency. In this study, we estimated genetic parameters and performed weighted single-step GWAS for five semen traits in RIR chickens to reveal their genetic architecture and identify key candidate genes and QTLs associated with semen quality. The results showed that heritability estimates for SEVOL, SECON, SPMOT, SPABR, and SPCOUNT ranged from 0.097 to 0.322, indicating low to moderate heritability. 29 significant QTL regions and 12 candidate genes (NFKB1, UBE2D3, PPP3CA, EIF4E, H2AFZ, DOCK2, MTNR1A, TACC3, ADCYAP1, GFRA1, ABLIM1, and CASP7) associated with semen traits were identified by WssGWAS. These QTL regions were located on chromosomes 4 and 13 for SEVOL, chromosome 4 for SPMOT, and chromosomes 2 and 6 for SPABR. Interestingly, four prominent consecutive QTL regions were shared between SEVOL and SPMOT. These QTL were located at 59.83-61.43 Mb, explaining 11.55% and 16.33% of the genetic variance for SEVOL and SPMOT, respectively. The largest-effect SNPs within these four QTL intervals exhibited significant effects on both semen volume and sperm motility, and these markers could be used for marker-assisted selection of semen traits. This study provides further insights into the genetic architecture of chicken semen traits, improves our understanding of their molecular regulation, and identifies valuable QTL and candidate genes. These findings offer a scientific basis for genetic improvement and marker-assisted selection of semen quality. - Source: PubMed
Publication date: 2026/05/28
Tesfay Hailai HagosZhang XiaokeLi YunleiZong YunheMa ZhongHan XintongZhao YiPan ZitingMani Adamu IsaYang FujianZhang ZongyaoChen JimingSun YanyanChen Jilan