Mouse CD80 _ B7-1 ELISA kit
- Known as:
- Mouse CD80 _ B7-1 Enzyme-linked immunosorbent assay test reagent
- Catalog number:
- BEK1031
- Product Quantity:
- 96 T
- Category:
- Elisa Kits
- Supplier:
- Biospect
- Gene target:
- Mouse CD80 _ B7-1 ELISA kit
Related genes to: Mouse CD80 _ B7-1 ELISA kit
- Gene:
- CD80 NIH gene
- Name:
- CD80 molecule
- Previous symbol:
- CD28LG, CD28LG1
- Synonyms:
- B7.1, B7-1
- Chromosome:
- 3q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 1993-12-14
- Date modifiied:
- 2016-10-05
Related products to: Mouse CD80 _ B7-1 ELISA kit
Related articles to: Mouse CD80 _ B7-1 ELISA kit
- Bacteria and their metabolic products profoundly affect the immune system. Research has shown that early postnatal supplementation with specific probiotic strains, such as Escherichia coli O83:K24:H31 (EcO83), can provide health benefits. EcO83 is a facultative anaerobe capable of generating energy through multiple metabolic pathways, an adaptation that allows it to thrive in the gastrointestinal tract, where oxygen availability can vary. However, the precise mechanisms through which this probiotic, and in particular its metabolites, functions remain largely unknown. Short-chain fatty acids (SCFAs) represent an important class of metabolites with known immunoregulatory effects. In this exploratory study, we aimed to test the impact of aerobic and anaerobic conditions on SCFA production and evaluate the immunomodulatory capacity of postbiotics derived from EcO83 under aerobic and anaerobic conditions on human peripheral blood mononuclear cells (PBMCs), mouse splenocytes, and bone marrow-derived dendritic cells (BMDCs) in vitro. Our results revealed that supernatants from aerobic cultures preferentially induced Th1 cytokines, such as IFN-γ, in PBMCs, whereas those from anaerobic cultures more prominently triggered the Th2 cytokine IL-13. Similarly, murine splenocytes produced more IFN-γ when stimulated with aerobic supernatants. Additionally, both aerobic and anaerobic EcO83 supernatants promoted the release of proinflammatory (TNF-α) and anti-inflammatory (IL-10) cytokines from BMDCs, as well as their maturation, evidenced by upregulation of the surface marker CD80. We detected SCFA, specifically acetate and propionate, in the supernatants of both aerobic and anaerobic EcO83 cultures, with no statistically significant differences between them. In conclusion, our findings suggest that probiotics may exert beneficial effects through the content of their secreted supernatants rather than solely through the presence of bacterial cells, highlighting the potential of such preparations and their component molecules as a postbiotic therapeutic solution. To our knowledge, this is the first report demonstrating that EcO83 produces SCFAs, including acetate and propionate, under different oxygen conditions. - Source: PubMed
Publication date: 2026/08/20
Černý VInic-Kanada AKrčmářová EProtivová ENovotná OPetrásková PSúkenı́ková LKuzma MSchabussova IHrdý J - Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system characterized by immune-mediated demyelination and neuroinflammation resulting from the breakdown of self-tolerance. Tolerogenic dendritic cells (tolDC) have emerged as a promising therapeutic strategy to restore antigen-specific immune tolerance; however, generating stable tolDC under inflammatory conditions remains challenging. Although interferon (IFN)-γ is traditionally regarded as a pro-inflammatory cytokine, accumulating evidence indicates that it can also exert protective and immunoregulatory functions in specific cellular and disease contexts. Here, we investigated whether low-dose IFN-γ directly programs dendritic cells (DC) toward a stable tolerogenic phenotype with therapeutic relevance for MS. - Source: PubMed
Publication date: 2026/08/05
Vilchez ConstanzaParra-Tello BrianGonzález Luis FNoriega VivianaCarrión FlavioPacheco RodrigoPrado CarolinaMartínez-Cáceres EvaGuevara CarlosNaves Rodrigo - Deficient IL10 signaling can contribute to macrophage activation syndrome. We hypothesized that in TLR-9-mediated inflammation, blocking IL10 would increase T cell activation and worsen organ dysfunction. - Source: PubMed
Publication date: 2026/08/05
Fisler GraceEremita MatthewChhabra ShivaniBrewer Mariana RQi MeiYuen Joyce HuiDeutschman Clifford STaylor Matthew D - Herpes zoster (HZ) is an infectious disease caused by the varicella zoster virus. Vaccination, especially recombinant protein vaccine, is an effective preventive method. However, the protein vaccine is easy to degrade, and its immunogenicity is weak, combined adjuvants and delivery systems can improve protein vaccines immune efficacy. In this work, a self-adjuvanted hydrogel vaccine (OHH/gE) was prepared by oxidized mannan and hydroxypropyl chitosan, which could slowly release antigen to continuously stimulate the body to improve immune level and effectively prevent and treat Hz. The results showed that this hydrogel (OHH) was injectable, able to gel in situ and slowly release antigens, protecting them from degradation. OHH, as a toll like receptor (TLR-4 agonist), causes the upregulation of costimulatory molecules (CD11c, CD80, CD86). After subcutaneous administration, OHH/gE significantly increased the antibody levels (IgG, IgG2a, IgG1) as well as the cellular immune levels (IL-2, IL-4, IL-10, IFN-γ). In addition, OHH/gE promoted lymphocyte activation and prolonged germinal centers, which was associated with high body fluid levels. Overall, this study designed a novel antigen delivery platform with adjuvant function, which showed great potential in the immunotherapy of diseases. - Source: PubMed
Publication date: 2026/08/17
Wang YuqinMeng TingtingXu HongxiaZhang QianYu NaPeng LiCai YaqinZuo WenbaoYang Jianhong - To investigate the roles of the α2,3-sialyltransferase ST3Gal1 and the α2,6-sialyltransferase ST6Gal1 in T cell-mediated tumor control, we generated mice with mature T cell-specific deletion of ST3Gal1 (T-ST3KO) or ST6Gal1 (T-ST6KO) using distal Lck-Cre-mediated recombination. Deletion of ST3Gal1 in mature T cells did not affect thymic T cell development but resulted in a reduction of peripheral CD8+ T cells. In contrast, ST6Gal1 deletion had minimal impact on T cell development and peripheral T cell abundance. Following in vitro stimulation of isolated T cells from T-ST3KO and parental wild type (WT) mice with anti-CD3 plus anti-CD28/CD80-Fc, CD8+ T cells from T-ST3KO mice exhibited enhanced activation and an increased frequency of CD44 positive memory-like T cells, while comparison of T cells from T-ST6KO and the parental WT mice showed no significant changes. Despite the activated CD8+ T cell phenotype in T-ST3KO mice, subcutaneous MC38 tumors displayed accelerated growth. In contrast, tumor progression in T-ST6KO mice was unchanged from the parental WT mice. Notably, T-ST6KO mice developed increased pulmonary metastases following intravenous challenge with B16F10 melanoma cells, whereas metastatic burden was unaffected in T-ST3KO mice. These findings demonstrate distinct and non-redundant roles for ST3Gal1- and ST6Gal1-mediated sialylation in regulating T cell function and antitumor immunity and reveal context-dependent effects of T-cell intrinsic sialylation in controlling primary tumor growth and metastatic dissemination. - Source: PubMed
Publication date: 2026/08/19
Wang XiaoshuangPaulson James C