Recombinant Human ISG15
- Known as:
- Recombinant Human ISG15
- Catalog number:
- CG40
- Product Quantity:
- 5x10ug
- Category:
- -
- Supplier:
- Novoprotein
- Gene target:
- Recombinant Human ISG15
Related genes to: Recombinant Human ISG15
- Gene:
- ISG15 NIH gene
- Name:
- ISG15 ubiquitin like modifier
- Previous symbol:
- G1P2
- Synonyms:
- IFI15, UCRP
- Chromosome:
- 1p36.33
- Locus Type:
- gene with protein product
- Date approved:
- 1990-10-16
- Date modifiied:
- 2019-04-23
Related products to: Recombinant Human ISG15
Related articles to: Recombinant Human ISG15
- Viral infections in the kidney activate innate immunity double-stranded RNA (dsRNA) sensors such as retinoic acid-inducible gene-I (RIG-I). This induces the expression of interferons and interferon-stimulated genes (ISGs), including ISG15. Similarly to ubiquitin, ISG15 functions by binding to target proteins and exerting antiviral effects through ISGylation. ISG15 is secreted extracellularly and exerts antiviral effects. Ubiquitin-like modifier-activating enzyme 7 (UBA7) initiates ISGylation, whereas ubiquitin-specific protease 18 (USP18) removes ISG15 from conjugated proteins. Both RIG-I and ISG15 are involved in antiviral responses and renal fibrosis. However, their interaction during kidney inflammation remains unclear. - Source: PubMed
Tachizaki MayukiKawaguchi ShogoTanaka HiroshiImaizumi Tadaatsu - Post-ovulatory supplementation of progesterone (P4) to cows has been associated with enhanced conceptus elongation. The aim of the present study was to investigate the impact of P4 supplementation on peri-implantation conceptus signaling including the secretion of interferon tau (IFNT), the maternal response to IFNT (mRNA abundance of interferon-stimulated genes [ISG; ISG15 and MX2]), and circulating pregnancy-associated glycoproteins (PAG). Two cohorts (A and B) of primiparous Angus beef cows were synchronized to estrus, artificially inseminated (d0), and assigned randomly to receive intravaginal P4 device (CIDR, 1.38 mg) from d3 to d12 or remain as untreated controls. Blood and cervical cytobrush were collected from d3 to d24 and a uterine cytobrush was collected on d24 from Cohort A to assess circulating concentrations of P4, PAG, mRNA abundance of ISG, and concentrations of IFNT, respectively. Blood and cytobrush (cervical and uterine) were collected from Cohort B on d18 to assess circulating concentrations of P4, mRNA abundance of ISG, and concentrations of IFNT, respectively. The mRNA abundance of ISG was assessed in luteal biopsies on d18 and d22. There was no effect of P4 supplementation on concentrations of IFNT in the uterus on d18. There was no effect of supplemental P4 on mRNA abundance or timing of ISG in endometrial, cervical, or luteal tissue. The earlier detection of PAG in circulation and lower uterine fluid concentrations of IFNT on d24 in P4-supplemented cows suggests advanced trophoblast differentiation and enhanced conceptus development beyond previously reported advances in conceptus elongation alone. - Source: PubMed
Publication date: 2026/04/28
Ehresmann Lillian Xda-Silva-Junior Florentino P JBaroody HollisDotts Gemma DKidwell Ellie GBonacim Paulo MBishop Jeanette VGuzeloglu AydinHansen Thomas RDomingues Rafael R - Osteoarthritis (OA) is a degenerative joint disease with high global prevalence, and YKL-40 is an important factor related to the pathological process of OA. Increased levels of YKL-40 exert a protective influence against TNF-α-induced apoptosis in chondrocytes, thereby enhancing chondrocyte survival and activation, while counteracting TNF-α-driven expression of specific inflammatory mediators such as S100A8/A9.This study aims to evaluate the role and molecular mechanism of YKL-40 on chondrocytes in OA and provide a potential therapeutic avenue requiring further validation. A meta-analysis compared serum YKL-40 and TNF-α levels between OA patients and healthy controls. In vitro experiments examined the effects of YKL-40 on TNF-α-induced OA chondrocytes, assessing proliferation, differentiation, apoptosis, and inflammatory pathways. Meta-analysis revealed significantly elevated serum levels of YKL-40 and TNF-α in osteoarthritis (OA) patients compared to healthy controls. In vitro, TNF-α (10 ng/mL) induced extracellular matrix (ECM) degradation in chondrocytes, significantly reducing glycosaminoglycan (GAG) and type II collagen content. This degradation was effectively rescued by YKL-40 (100 ng/mL). RNA sequencing identified differentially expressed genes in TNF-α-treated chondrocytes, enriched in pathways like IL-17 and NF-κB signaling. YKL-40 treatment reversed the expression of key genes altered by TNF-α. Crucially, these differentially expressed genes (including S100A8/A9, ISG15, CDSN, BAAT, PTPN4, NPTX1, SMARCA1) were validated in independent OA cartilage and synovium GEO datasets. Protein-protein interaction (PPI) networks highlighted central genes within treatment groups. Western blotting confirmed YKL-40 counteracted TNF-α-induced NF-κB pathway activation (reduced p65 and IκBα phosphorylation) and modulated key targets (S100A8/A9, ASB7, ZFPM2), consistent with qRT-PCR data. YKL-40 is a promising biomarker and therapeutic target for OA. Its interplay with TNF-α provides a molecular basis for novel therapies targeting chondrocyte dysfunction, guiding future translational research. - Source: PubMed
Publication date: 2026/04/27
Li ZhuozhengSun XueXie YongfangHong ZexinDou ZeminYan ShichaoZhang YulongQin HeLiu DanFeng TingtingWang Guohui - The maturation of oocytes is a critical step in mammalian reproduction, involving dynamic regulation of gene expression. Therefore, investigating how gene expression varies during different stages of oocyte maturation is highly important. This study employed single-cell RNA sequencing (scRNA-seq) to analyze bovine oocytes at the germinal vesicle (GV) and metaphase II (MII) stages. The results identified 1787 differentially expressed genes (DEGs) between the two stages, with 1556 genes upregulated and 231 downregulated in the GV stage. Further investigation using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that the upregulated genes are mainly involved in mitochondrial functions and energy metabolism, whereas the downregulated genes are primarily associated with signaling pathways. Validation through RT-qPCR confirmed that , and were significantly higher in GV-stage oocytes, while , and were notably downregulated. This study highlights significant gene expression differences between GV and MII bovine oocytes, underscoring the vital roles of genes related to energy metabolism and signaling during oocyte maturation. The expression patterns of these genes provide important molecular markers for further elucidating the mechanisms underlying oocyte maturation. - Source: PubMed
Publication date: 2026/04/21
Wang XueyanHuang FeiLi XiaopengHu KaiChen HongNiu PengQu HuiminFang DiHan ChunmeiGao Qinghua - Bovine viral diarrhea virus (BVDV) strains of differing virulence are associated with distinct clinical and lymphoid outcomes, but transcriptional responses within peripheral lymphocyte compartments remain incompletely defined. Here, we performed RNA-seq on CD4/CD8β/TcR1 bead-enriched peripheral lymphocyte fractions from calves experimentally infected with low-virulence or high-virulence noncytopathic BVDV strains (BVDV2-RS886 and BVDV2-1373, respectively) and sampled at days 0, 3, and 15 days post-inoculation. Because group sizes were small (n = 3 per group), analyses were interpreted as exploratory. Marker-gene analyses indicated that the sequenced material represented a T-cell-enriched but compositionally heterogeneous peripheral lymphocyte fraction. At day 3, both infection groups showed strong induction of interferon-responsive genes, including OAS family members, ISG15, IFI44/IFI44L, RSAD2, DDX58, and ZBP1. The high-virulence group additionally showed broader reduction of transcripts associated with lymphocyte signaling, trafficking, and biosynthetic activity. By day 15, the low-virulence group showed only a small residual response, whereas the high-virulence group remained broadly perturbed, with reduced abundance of multiple adaptive immune and antigen-presentation-associated transcripts in the bead-enriched fraction. These data define divergent temporal transcriptomic trajectories associated with BVDV2 virulence and provide a hypothesis-generating resource for future studies using phenotypically defined bovine lymphocyte subsets. - Source: PubMed
Publication date: 2026/04/23
Bauermann Fernando VicosaBayles Darrell OFalkenberg Shollie MMaggioli MayaraRidpath Julia F