AKR1C3 _ DDH3 Rabbit antibody Ab Aff - Purified
- Known as:
- AKR1C3 _ DDH3 Rabbit (anti-) Antibody Aff - Purified
- Catalog number:
- GTX113586
- Product Quantity:
- 0.1 ml
- Category:
- -
- Supplier:
- ACR
- Gene target:
- AKR1C3 _ DDH3 Rabbit antibody Aff - Purified
Related genes to: AKR1C3 _ DDH3 Rabbit antibody Ab Aff - Purified
- Gene:
- AKR1C3 NIH gene
- Name:
- aldo-keto reductase family 1 member C3
- Previous symbol:
- HSD17B5
- Synonyms:
- KIAA0119, DDX, HAKRB, PGFS
- Chromosome:
- 10p15.1
- Locus Type:
- gene with protein product
- Date approved:
- 1998-09-29
- Date modifiied:
- 2016-10-05
Related products to: AKR1C3 _ DDH3 Rabbit antibody Ab Aff - Purified
Related articles to: AKR1C3 _ DDH3 Rabbit antibody Ab Aff - Purified
- The potential contribution of foodborne pesticide residues to colorectal cancer (CRC) remains insufficiently understood. In this study, we integrated computational toxicology, network analysis, machine learning, molecular docking, and in vitro validation to investigate possible molecular links between representative foodborne pesticides and CRC. The toxicological properties of ten pesticides were evaluated using ADMETlab 3.0 and ProTox-III. Pesticide-associated target genes were predicted using TargetNet, SuperPred, and the Similarity Ensemble Approach and were intersected with CRC-related genes obtained from GeneCards and OMIM. Functional enrichment analysis showed that the overlapping genes were enriched in xenobiotic response, oxidative stress, and cancer-related pathways. Using random survival forest and least absolute shrinkage and selection operator regression, six hub genes, including PDGFRA, AKR1C3, PDGFRB, CDC42, PIK3CA, and CYP2C9, were identified and used to construct a prognostic model with favorable predictive performance. Among these candidates, AKR1C3 exhibited strong predicted binding affinity with several pesticides, particularly cypermethrin and deltamethrin. Cellular thermal shift assay further supported a direct interaction between cypermethrin and AKR1C3. Cypermethrin reduced cell viability, DNA synthesis, and AKR1C3 protein expression in NCM460 cells. To improve CRC relevance and functional validation, HCT116 colorectal cancer cells were further included. Cypermethrin decreased HCT116 cell viability and AKR1C3 expression, whereas AKR1C3 overexpression partially restored cypermethrin-induced viability reduction and attenuated intracellular ROS accumulation. Together, these findings identify AKR1C3 as a candidate functional target of cypermethrin and suggest that AKR1C3 may be involved, at least in part, in cypermethrin-induced CRC-relevant cellular injury and oxidative stress. This study provides an exploratory framework for identifying exposure-related molecular targets linking foodborne pesticide residues to CRC-associated biological alterations. - Source: PubMed
Publication date: 2026/06/03
Xu HouxiLi XiaoxiaoSun SongxianWang XiaolongLi Yu - The placenta produces a variety of steroid hormones through the catalytic activity of steroidogenic enzymes, including cytochrome P450 (CYP) hydroxylases and hydroxysteroid dehydrogenases (HSD). Large amounts of progesterone produced by the placenta are essential for the maintenance of pregnancy. Although androgens and estrogens are also elevated in maternal circulation during gestation, there are conflicting reports on whether de novo synthesis of these steroids occurs in the human placenta. To address this issue, we performed a comprehensive analysis of steroidogenic gene expression in early and term placenta. While none of the genes examined showed binary expression changes, 17β-HSDs, including HSD17B1 and AKR1C3, were markedly upregulated in the term placenta. CYP19A1 and HSD11B2 genes were also markedly upregulated. In contrast, CYP17A1, CYP21A2, CYP11B1, CYP11B2, and HSD17B3 were almost undetectable. Consistent with these findings, the plasma ratios of active to precursor sex steroids (estradiol/estrone and testosterone/androstenedione) were higher in pregnant than in non-pregnant women, although concentrations of all steroids increased. In contrast, plasma levels and profiles of 11-oxygenated androgens were unchanged. These results indicate that the human placenta does not significantly contribute to circulating levels of either classical or novel classes of androgens. Therefore, this study provides new insights into the tissue of origin and the physiological significance of sex steroids during gestation. - Source: PubMed
Publication date: 2026/05/12
Yokohama YukoWatanabe YugoNakajima Ke-IchiUmezawa AkihiroTakahashi SatoruMori YasuhiroKato YasuhitoKawabe Jun-IchiYazawa Takashi - Breast cancer remains the most prevalent cancer among women, driving the continuous search for novel anticancer agents. In this study, a series of 10-alkoxy steroids containing a 1,4-dien-3-one moiety was synthesized via a one-step dearomatization of the A-ring of estradiol and an estrane derivative with a D-homo lactone moiety. All compounds exhibited favorable in silico ADMET properties, while five derivatives showed significant cytotoxic activity in vitro. The strongest effects against the estrogen-positive breast cancer cell line MCF-7 were observed for two 10β-methoxy, one 10β-butoxy, and two 10β-propargyloxy derivatives. The activity of the 10β-butoxy-17β-hydroxy and 10β-propargyloxy-17β-hydroxy steroids correlates with strong binding affinity toward estrogen receptor α and inhibition of AKR1C3 and AKR1C4 enzymatic activity. Additionally, the propargyloxy derivative showed substrate-like binding to human recombinant aromatase. Molecular docking results are in qualitative agreement with these findings, predicting binding modes similar to known ligands and possible hydrophobic interactions between the C-10 alkoxy groups and the active sites of target proteins, particularly the heme group of aromatase. These results highlight novel estrane derivatives as promising candidates for estrogen-positive breast cancer therapy through combined effects involving AKR1C3 inhibition and interaction with aromatase and estrogen receptor. - Source: PubMed
Kuzminac IvanaBekić SofijaStevanović MilicaScholda JuliaKopp FlorianPetri EdwardĆelić AnđelkaSakač Marija - Arachidonic acid (AA), a membrane-abundant polyunsaturated fatty acid, is primarily liberated from membrane phospholipids by phospholipase A (PLA), and is subsequently metabolized into bioactive eicosanoids involved in vascular tone and inflammation. With lipidomics advances, AA metabolism's multifaceted roles in the tumor microenvironment (TME) have emerged, and it is recognized as a key driver and potential therapeutic axis in lung adenocarcinoma (LUAD). We utilized spatial transcriptomics sequencing (ST-seq) and LUAD-associated single-cell RNA sequencing (scRNA-seq) to explore crucial AA-related biomarkers in LUAD. - Source: PubMed
Publication date: 2026/05/19
Sun ChongqiZhang YuchenPan YunXia GuixiYang GuangrongHuang ChunkaiLi JunMa Pei - Hepatocellular carcinoma (HCC) is one of the most prevalent malignant tumors globally, with liver cirrhosis (LC) recognized as a significant precursor. Xenobiotic metabolism plays a pivotal role in liver diseases, where the liver's primary function as a detoxifying organ directly influences health and tumor development. Therefore, exploring the function of genes associated with xenobiotic metabolism in patients with HCC and LC is crucial for advancing diagnosis and treatment strategies. - Source: PubMed
Publication date: 2026/05/13
Xu HaoLi YanpengZhao RuiMa ShaoweiHu NingYu ShuoLv Xuefeng