Human Activating Transcription Factor 3 ELISA , ATF3
- Known as:
- Human Activating Transcription Factor 3 Enzyme-linked immunosorbent assay test , ATF3
- Catalog number:
- E01A0533
- Product Quantity:
- 96 Tests/kit
- Category:
- -
- Supplier:
- BGene
- Gene target:
- Human Activating Transcription Factor 3 ELISA ATF3
Related genes to: Human Activating Transcription Factor 3 ELISA , ATF3
- Gene:
- ATF3 NIH gene
- Name:
- activating transcription factor 3
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1q32.3
- Locus Type:
- gene with protein product
- Date approved:
- 1994-08-03
- Date modifiied:
- 2014-11-19
Related products to: Human Activating Transcription Factor 3 ELISA , ATF3
Related articles to: Human Activating Transcription Factor 3 ELISA , ATF3
- The global incidence of thyroid cancer is rising, yet its underlying molecular etiology remains elusive. This study aimed to investigate the roles of transcription factor ATF3 and miR-27b-3p in thyroid cancer. Bioinformatics analysis identified miR-27b-3p as the upstream regulatory miRNA of ATF3. In thyroid cancer, ATF3 was found to be downregulated, whereas miR-27b-3p was upregulated, and both were associated with aggressive tumor features. Their expression in clinical thyroid cancer samples was measured via qRT-PCR and immunohistochemistry (IHC), which confirmed decreased ATF3 expression and increased miR-27b-3p levels. Higher miR-27b-3p expression was associated with C-TIRADS classification, TNM stage, and serum TSH levels. A dual-luciferase reporter assay confirmed that miR-27b-3p directly targets ATF3, and inhibiting miR-27b-3p markedly increased ATF3 expression. Additionally, functional experiments demonstrated that overexpressing miR-27b-3p or knocking down ATF3 enhanced thyroid cancer cell proliferation, migration, and invasion. Conversely, inhibiting miR-27b-3p or overexpressing ATF3 suppressed these malignant phenotypes, while simultaneous silencing of miR-27b-3p and ATF3 partially rescued the inhibitory effects. These findings suggest that miR-27b-3p enhances thyroid cancer cell aggressiveness in part by inhibiting ATF3, providing insights that warrant further investigation. - Source: PubMed
Publication date: 2026/07/06
Lan HuihuaZeng JianghuiXiong YuMo Wuning - Interactions between sensory neurons and tumor cells are increasingly recognized as critical contributors to tumor progression and cancer-associated pain. Here, we describe a reproducible protocol for the isolation and culture of adult mouse dorsal root ganglion (DRG) neurons, followed by functional and molecular assays to assess their responses to tumor-derived secreted factors. Adult DRG were dissected, enzymatically digested with Liberase DH, and dissociated into single neurons. The cells were plated on laminin-coated coverslips and maintained in defined culture conditions that promote neuronal survival while minimizing glial overgrowth. Subsequently, the cultured neurons were exposed to tumor cells' conditioned medium to model paracrine signaling at the neuron-tumor interface. Functional responses were monitored by live cell calcium imaging using fluorescent indicators, enabling quantification of neuronal activity and stimulus-specific responsiveness. In parallel, neuronal stress and injury are evaluated by ATF3 immunostaining. Together, these complementary approaches provide a powerful platform to investigate how tumor-secreted factors sensitize or activate sensory neurons. This protocol integrates adult DRG culture, tumor-conditioned medium treatment, calcium imaging, and molecular readouts into a single workflow, enabling researchers to dissect mechanisms of neuron-tumor communication. The method is broadly applicable to studies of cancer neuroscience, pain signaling, and the identification of secreted mediators that modulate neuronal function in disease contexts. - Source: PubMed
Publication date: 2026/06/22
Kasm Khalil Ali AhmadDembla SandeepHirbe Angela CShepherd Andrew JPan Yuan - To investigate the contribution of endoplasmic reticulum (ER) stress to the progression of thyroid cancer (TC) and to construct a prognostic gene signature. - Source: PubMed
Publication date: 2026/07/06
Wang MinGuo Xiao-PengYuan Zi-BoGuo Ya-WenZheng Guo-WanLiang Rui-MinMa JieWang Jia-QiWang Xiao-GangSong Fa-HuanZhu XinJin Lu-Qiang - Intramuscular fat (IMF) is a key indicator of pork quality. Laiwu pigs represent a valuable model for studying IMF deposition due to their extremely high IMF content, yet the regulatory mechanisms of chromatin accessibility in skeletal muscle remain unclear. To dissect the epigenetic and transcriptional mechanisms underlying IMF deposition, we performed integrative ATAC-seq and RNA-seq analysis on longissimus thoracis (LT) muscle of six pigs with extreme IMF phenotypes, selected from a population of 79 Laiwu pigs. - Source: PubMed
Publication date: 2026/07/02
Li JingxuanWang JiyingWang YanpingMao RuihanZhao Xueyan - Chronic kidney disease (CKD) affects approximately 10% of global populations, pathologically characterized by renal fibrosis including extracellular matrix (ECM) deposition and renal tubular atrophy. Emerging evidence suggests that autophagy plays a crucial role in renal fibrosis, but the regulatory mechanisms are still not well understood. - Source: PubMed
Publication date: 2026/07/01
Yan RuiweiSong AnniHu MingcunYin XingjieXiong WeiGao PanZhang Chun