PUM2 Antibody (S182)
- Known as:
- PUM2 Antibody (S182)
- Catalog number:
- AP7734d
- Category:
- -
- Supplier:
- Abgen
- Gene target:
- PUM2 Antibody (S182)
Related genes to: PUM2 Antibody (S182)
- Gene:
- PSEN1 NIH gene
- Name:
- presenilin 1
- Previous symbol:
- AD3
- Synonyms:
- FAD, S182, PS1
- Chromosome:
- 14q24.2
- Locus Type:
- gene with protein product
- Date approved:
- 1992-11-05
- Date modifiied:
- 2019-04-23
- Gene:
- PUM2 NIH gene
- Name:
- pumilio RNA binding family member 2
- Previous symbol:
- -
- Synonyms:
- PUMH2, KIAA0235
- Chromosome:
- 2p24.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-03-27
- Date modifiied:
- 2015-11-05
- Gene:
- RNA5SP182 NIH gene
- Name:
- RNA, 5S ribosomal pseudogene 182
- Previous symbol:
- RN5S182
- Synonyms:
- -
- Chromosome:
- 5q11.2
- Locus Type:
- pseudogene
- Date approved:
- 2011-10-24
- Date modifiied:
- 2014-11-19
Related products to: PUM2 Antibody (S182)
Related articles to: PUM2 Antibody (S182)
- Translational control is essential for male germ cell development, yet how post-transcriptional regulation is coupled to chromatin remodeling during spermatogenesis remains poorly understood. Here, we show that the RNA-binding proteins PUMILIO1 (PUM1) and PUMILIO2 (PUM2) promote translation of mRNAs encoding chromatin regulators in the mouse testis. Conditional deletion of and in germ cells at multiple developmental stages results in spermatogenic failure, defective nuclear shaping, impaired histone-to-protamine exchange, and complete male sterility. Polysome profiling and ribosome sequencing reveal selective reductions in translation efficiency of chromatin regulators, including histone modifiers and nucleosome remodelers, in -deficient testes. Mechanistically, PUM1/2 associate with DAZL and PABPC1 to form a germ cell-specific translational activation complex that enhances protein output with little impact on mRNA stability. Together, these findings redefine mammalian PUM proteins as context-dependent translational activators in the germline and uncover a post-transcriptional mechanism that links RNA regulation to chromatin dynamics during spermatogenesis. - Source: PubMed
Publication date: 2026/07/17
Zang MinLiu SiyuXie ZhengyaoYang DingWang KeDing YanZhu TongZhang ShikunZhao TingtingYao BingLiu MingxiLin KaiboXu Eugene Yujun - Pumilio proteins (PUM1 and PUM2) are evolutionarily conserved RNA-binding proteins that regulate gene expression at the post-transcriptional level and have been implicated in tumourigenesis in various cancers. However, their role in gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) has not been previously investigated. This study aimed to evaluate the expression of PUM1 and PUM2 in GEP-NENs and determine their association with tumour grade and metastatic status. Transcriptomic data (GSE98894) were analyzed using GEO2R to assess differential gene expression in primary tumours, lymph node metastases, and distant meta- stases. Additionally, immunohistochemical analyses were performed on formalin- fixed paraffin-embedded samples, and protein expression was quantified using digital image analysis. Statistical comparisons were conducted across tumour sites and grades (G1-G3). At the mRNA level, PUM2 was significantly upregulated in distant metastases compared with primary tumours (log2FC = 0.151; adjusted p = 0.024), whereas PUM1 showed no significant difference. Protein-level analyses confirmed expression of both proteins in all samples; however, only PUM2 was significantly elevated in high-grade (G3) tumours compared to G1/G2. These findings suggest that PUM2, but not PUM1, is associated with tumour progression in GEP-NENs, although a causal role remains to be established. - Source: PubMed
Kraiński PatrykKluk AndrzejGruszczynski JanTrębacz KacperDwojak EwaDorota MaciejKołodziejski Paweł AntoniWozniak Aldona Wiesława - Long non-coding RNAs (lncRNAs) are increasingly implicated in neurodegenerative disease, yet their roles in tauopathy remain poorly understood. Here, we defined the lncRNA landscape across iPSC-derived neurons, astrocytes, and microglia harboring the frontotemporal dementia-associated IVS10+16 mutation and investigated how lncRNA dysregulation interfaces with tau pathology. Transcriptomic analyses revealed extensive cell-type specific lncRNA expression changes, with neurons exhibiting the greatest degree of mutation-associated remodeling. Comparative analyses with IVS10+16 patient brain tissue identified and as lncRNAs significantly dysregulated across all three cell types and human brains. was also altered in Alzheimer's disease and Parkinson's disease brains, suggesting a broader role in neurodegenerative disease. Mechanistically, -associated protein networks converged on pathways related to RNA regulation, cytoskeletal organization, proteostasis, and tau interaction networks. Given the established role of in regulating PUM1 and PUM2 RNA-binding (pumilio) proteins, we examined the -pumilio axis and identified enrichment of pumilio-associated pathways linked to autophagy, endocytosis, proteostasis, and cytoskeletal regulation. depletion reduced tau seeding and uptake, whereas functional depletion of or increased both processes, supporting an antagonistic relationship between and pumilio signaling in modulation of tau aggregation. Together, these findings identify widespread lncRNA dysregulation across neural cell types in the setting of a mutation and nominate the -pumilio axis as a regulatory pathway linking RNA homeostasis and tau propagation biology. - Source: PubMed
Publication date: 2026/07/03
Zemke Joseph EHuang GuangmingStarr EmmaBroder MatthewMarsh JacobRenganathan ArunPhillips BridgetMarsh Thomas WMinaya MiguelCruchaga CarlosIyer Abhirami K Karch Celeste M - Loss of the RNA-binding proteins PUM1 and PUM2 leads to gastrulation failure and embryonic lethality, but the mechanisms underlying this phenotype remain unclear. Using embryoid bodies as an in vitro model of early embryogenesis, we show that PUM proteins maintain lineage balance by post-transcriptionally repressing germline fate, silencing pluripotency networks, and promoting somatic differentiation. They directly inhibit Prdm1 to prevent premature germline specification and activate Wnt signaling to support germ layer formation. PUM-deficient cells exhibit impaired patterning along both the anterior-posterior and dorsoventral axes, along with defective neural differentiation. RIP-seq analyses identify key developmental mRNAs directly regulated by PUM1 and PUM2, linking these proteins to multiple regulatory modules. These findings establish PUM proteins as essential post-transcriptional regulators of early embryogenesis, demonstrating how RNA-binding proteins coordinate cell fate and patterning. - Source: PubMed
Publication date: 2026/07/09
Lu TingYang YiyingLi XiajunShi Shuo - Sevoflurane preconditioning (SPC) is a common protective strategy against myocardial ischemia-reperfusion injury (MI/RI). MIR210HG is a hypoxia-induced long non-coding RNA and involves multiple molecular pathways. - Source: PubMed
Publication date: 2026/07/01
Gan LuDu XiaobingLu LuWang XiaomingChai Fei