SERPINF1 Antibody (Center)
- Known as:
- SERPINF1 Antibody (Center)
- Catalog number:
- AP7315c
- Category:
- -
- Supplier:
- Abgen
- Gene target:
- SERPINF1 Antibody (Center)
Related genes to: SERPINF1 Antibody (Center)
- Gene:
- SERPINF1 NIH gene
- Name:
- serpin family F member 1
- Previous symbol:
- PEDF
- Synonyms:
- EPC-1, PIG35
- Chromosome:
- 17p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1993-05-18
- Date modifiied:
- 2018-03-02
Related products to: SERPINF1 Antibody (Center)
Related articles to: SERPINF1 Antibody (Center)
- Pigment epithelium-derived factor (PEDF) promotes photoreceptor survival through its receptor PEDF-R, a phospholipase involved in retinal lipid metabolism. To define the in vivo function of the PEDF/PEDF-R axis, we generated mice lacking (PEDF) and (PEDF-R). Combined loss of and resulted in severe retinal degeneration characterized by outer nuclear layer (ONL) thinning, outer segment (OS) shortening, reduced rhodopsin and cone opsin expression, increased TUNEL-positive nuclei, and enhanced retinal autofluorescence associated with altered lipid distribution. Lipid-associated markers, including TIP47, PLIN5, and BODIPY, exhibited abnormal distribution patterns in mutant retinas, indicating disrupted lipid storage and trafficking. Loss of PEDF/PEDF-R signaling also impaired photoreceptor-rod bipolar cell connectivity, as demonstrated by reduced PKCα/synaptophysin colocalization, and resulted in diminished electroretinographic responses. Lipid Imaging mass spectrometry revealed decreases in some lipid abundances in photoreceptor outer segment and inner segment/outer nucleus layer, while lipids containing arachidonic acid and docosahexaenoic acid-containing lipids showed increased abundance. Together these findings identify the PEDF/PEDF-R signaling axis as a key regulator of retinal phospholipid homeostasis that couples lipid metabolism to photoreceptor survival and visual function. - Source: PubMed
Publication date: 2026/08/09
Bernardo-Colón AlexandraCrawford Susan EAgbaga Martin PaulWang ZhenSchey KevinBecerra S Patricia - In recent years, chilled chicken has emerged as a new market trend. Skin feather follicle density, an important carcass appearance trait characterized by "fine and dense" features, is increasingly preferred by consumers. This study analyzed hair follicle density in the leg and back regions of 1,382 yellow-feathered broiler. The results showed that back feather follicle density was significantly higher than that of the leg (P < 0.01), with a significant positive correlation between the two regions. Meanwhile, feather follicle density in both the leg and back showed negative correlations with abdominal fat weight, body weight, dressed weight, full-eviscerated weight, semi-eviscerated weight. mRNA-seq analysis of leg and back skin tissues from four individuals identified differentially expressed genes (DEGs) affecting feather follicle density traits across different anatomical locations, including GTF2A1, RPSA2, COX3, and CNKSR2. Furthermore, Genome-Wide Association Study (GWAS) analysis of 505 chickens identified 223 single-nucleotide polymorphisms (SNPs) significantly associated with leg feather follicle density and 107 SNPs associated with back feather follicle density, including candidate SNPs such as C19014967G, C17514817T, G1847078C, and C34389948G, as well as candidate genes including ELF5, MAP3K1, HIF1AN, and SERPINF1. mRNA-seq analysis of skin tissues from high and low feather follicle density groups in the leg (TG vs. TD) and back (BG vs. BD) identified 541 leg-related DEGs and 1,130 back-related DEGs, respectively. Joint analysis of TG vs. TD and BG vs. BD revealed 174 commonly DEGs, including candidate genes such as NSA2, IGF2, RPL17, and NRP1. Through integrated GWAS and RNA-seq analysis, five genes significantly associated with leg feather follicle density and twelve candidate genes associated with back feather follicle density were identified. Notably, individuals with the AA genotype at the SERPINF1 SNP locus Chr19:5636537 showed extremely significantly higher feather follicle density than those with the GG genotype, and both mRNA and protein expression levels of SERPINF1 were significantly upregulated in high feather follicle density individuals. - Source: PubMed
Publication date: 2026/07/31
Chao XiaohuanChen ShuyaOuYang TongYe ChutianChen JieWu JiongwenMa XueRongLiu AijunLiang WeimingCui TianxiXia LuluXiao KaifanHe YouchengZhang XiquanFang ChengLuo Qingbin - To describe the clinical symptoms, obesity status and body composition distribution in children with osteogenesis imperfecta (OI). This retrospective cohort study included 45 children aged 0-18 years with OI, diagnosed by clinical and genetic testing at Children's Hospital, Zhejiang University School of Medicine, from October 2020 to February 2025. Clinical data were collected on age, sex, symptoms, imaging examinations and genetic testing results. According to body mass index (BMI) criteria or weight-for-length Z-score, the OI patients were divided into non-overweight or obese group and overweight or obese group. Concurrently, 60 children with simple overweight or obesity, matched for sex, age and BMI, were enrolled as a control group. The -test and Mann-Whitney test were used to compare the clinical characteristics between the 2 OI groups, and body composition (lean mass index (LMI)and fat mass index (FMI)) between the overweight or obese OI group and the control group. Fracture frequency distribution between the two OI groups were compared using the ² test. Of the 45 OI children, 26 were male and 19 female, with an average age of (7.2±3.9) years. Genetic variants involved COL1A1 or COL1A2 in 40 cases, IFITM5 in 3, SERPINF1 in 1, and BMP1 in 1. Of the OI patients, 18 (40%) were in the overweight or obesity group and 27 (60%) in the non-overweight or obesity group; 19 (42%) had short stature or growth retardation. Between the 2 OI groups, none of sex, osteocalcin, bone mineral density Z-score, or vitamin D level differed significantly (all >0.05), whereas fracture frequency did differ significantly (<0.05). Twenty seven OI children (13 in the overweight or obesity group and 14 in the non-overweight or obesity group) completed the dual-energy X-ray absorptiometry, and all of them had COL1A1 or COL1A2 genotypes. The FMI level was higher in the overweight or obese group than in the non-overweight or obese group (9.0 (6.1, 9.6) 4.4 (3.7, 5.6) kg/m², =3.21, =0.005), whereas LMI and abdominal-to-gluteal fat percentage ratio did not differ significantly (both >0.05). In the OI overweight or obesity group, both FMI and LMI were lower than in the control group (9.0 (6.1, 9.6) 10.4 (9.2, 11.7) kg/m², 12.1 (11.7, 12.9) 13.9 (13.0, 15.1) kg/m², =3.45,3.72, both <0.05). Overweight or obesity was prevalent among children with OI and was closely associated with fracture frequency. Among those with COL1A1 or COL1A2 variants, the overweight or obese OI children was characterized by higher FMI and lower LMI. - Source: PubMed
Xu X QZheng J PZhou X LLin HChen X FYuan THuang KDong G PChen J SFu J FWu W - Adipose-derived stem cells (ASCs) possess substantial regenerative potential, with lineage fate tightly coupled to metabolic dynamics. In prior work, we found that expression of the tardigrade-derived protein Mitochondrial Abundant Heat Soluble (MAHS) increased stress tolerance and promoted ASC osteogenic differentiation over adipogenic differentiation, although the mechanism remained unclear. Here, we leverage transcriptomic and functional assays to investigate how MAHS expression influences ASC metabolism. Transcriptomic profiling revealed incomplete adipogenic activation alongside upregulation of ossification-associated genes (e.g., ), as well as broader changes in metabolic, Wnt, and YAP signaling pathways consistent with a shift toward osteogenic programming. Functionally, MAHS-expressing ASCs exhibited increased matrix calcium deposition in osteogenic culture, as well as decreased ATP and cAMP levels during basal culture. Further, treatment with forskolin increased cAMP levels, while forskolin or Compound C increased lipid accumulation in MAHS-expressing ASCs. Together, these findings suggest that MAHS expression perturbs metabolic dynamics and signaling networks in ASCs and may contribute to impaired adipogenesis and reinforcement of osteogenic bias. More broadly, this work highlights potential regulatory points that could be targeted to restore lineage balance in metabolically disrupted ASCs. - Source: PubMed
Publication date: 2026/07/15
Rolsma Jordan LLi JulieannaSoh RuthiaNieh ConnorYeh BryceDarch WilliamNieden Nicole ZurMorgan Joshua T - Neonatal calf health critically depends on successful passive transfer of immunity (PTI) via colostrum. Although PTI is routinely assessed by serum IgG concentration, associated systemic proteomic responses remain poorly defined. This study aimed to relate distinct PTI categories to neonatal calf serum proteomes and to evaluate colostrum proteomes to identify proteins influencing passive transfer success. Forty Holstein cow-calf pairs were enrolled. Calf serum was collected before colostrum feeding (0 h) and at 48 h. Serum IgG was quantified by ELISA, and calves were classified at 48 h as failed (FPTI, <1000 mg/dL, n = 11), adequate (APTI, 1000-2000 mg/dL, n = 11), or excellent (EPTI, >2000 mg/dL, n = 18). For proteomic analyses, five calves were purposefully selected in each group; serum samples were pooled by PTI group and time point, and colostrum samples by PTI group, then analyzed by LC-MS/MS in DIA mode. Proteomics identified 54 proteins in colostrum and 80 in serum, 27 of which were shared between matrices. Post-colostrum intake, 21 (FPTI), 5 (APTI), and 30 (EPTI) serum proteins changed significantly from 0 to 48 h. In EPTI calves, haptoglobin (Hp) (51.10-fold) and pigment epithelium-derived factor (PEDF) (27.24-fold) showed the strongest upregulation. At 48 h, lipopolysaccharide-binding protein (LBP) and haemoglobin subunit epsilon (Hb-ε) were lower in EPTI, whereas PEDF, polymeric immunoglobulin receptor (PIGR), and SERPINA3-6 were higher, consistent with coordinated activation of acute-phase, mucosal/transport, and protease-antiprotease pathways. These findings indicate that PTI involves coordinated proteomic changes beyond IgG alone and highlight candidate biomarkers of successful passive immunity in neonatal calves. - Source: PubMed
Publication date: 2026/07/17
Kaçar YiğitTopal OnurMecitoğlu ZaferSağlam NezaketFthenakis George CBatmaz Hasan