Mouse Camk2a Differentiation Reporter (pGreenZeo, Virus)
- Known as:
- Mouse Camk2a Differentiation Reporter (pGreenZeo, Virus)
- Catalog number:
- SR10022VA-1
- Product Quantity:
- >2 x 10^6 IFUs
- Category:
- -
- Supplier:
- SBI
- Gene target:
- Mouse Camk2a Differentiation Reporter (pGreenZeo Virus)
Related genes to: Mouse Camk2a Differentiation Reporter (pGreenZeo, Virus)
- Gene:
- CAMK2A NIH gene
- Name:
- calcium/calmodulin dependent protein kinase II alpha
- Previous symbol:
- CAMKA
- Synonyms:
- KIAA0968, CaMKIINalpha
- Chromosome:
- 5q32
- Locus Type:
- gene with protein product
- Date approved:
- 1993-11-24
- Date modifiied:
- 2016-04-06
Related products to: Mouse Camk2a Differentiation Reporter (pGreenZeo, Virus)
Related articles to: Mouse Camk2a Differentiation Reporter (pGreenZeo, Virus)
- Sevoflurane is one of the most commonly used general anesthetics in pediatric clinical practice worldwide. Although accumulating preclinical evidence indicates that neonatal sevoflurane exposure causes persistent cognitive impairments, the extracellular mechanisms remain unclear. Herein, we focus on perineuronal nets (PNNs), extracellular matrix (ECM) structures that constrain neuronal excitatory plasticity and are highly enriched in the hippocampal CA2, a region critical for social recognition memory. Neonatal mice repeatedly exposed to 3% sevoflurane (2 h/day, P6-P8) exhibited persistent CA2-specific PNN degradation in adulthood. Mechanistically, sevoflurane depleted H3K27me3 at the Mmp9 promoter, elevating matrix metalloproteinase 9 (MMP9) expression. This epigenetic dysregulation impaired BDNF/ TrkB signaling, reduced PSD-95 puncta density and dendritic spine abundance, and suppressed mEPSC frequency, leading to impaired novel object recognition and social discrimination. Spatial transcriptomics validated CA2-specific ECM pathway dysregulation. Notably, CA2-targeted Hapln1 overexpression in the CA2 excitatory neurons of Camk2a-Cre mice effectively restored PNN integrity, rescued synaptic dysfunction, and reversed cognitive deficits. The pharmacological inhibition of MMP9 yielded comparable neuroprotective effects. Collectively, this study identified PNNs as pivotal mediators of anesthetic neurotoxicity, uncovered a previously unrecognized epigenetic-ECM coupling mechanism driving developmental brain injury, and highlighted PNN preservation as a promising translational strategy for preventing pediatric anesthesia-associated cognitive impairment. - Source: PubMed
Publication date: 2026/09/29
Liang LirongWang JiachenWang TaozhiLi YanlingZhao YouyiHao GuanghuiGong PeiqinChen RulingFang YilinWu ShengxiZhang HaopengZhang Hui - Chaihu Shugan San (CSS), a classical seven-herb formula from the Ming-dynasty text Jing-Yue Quanshu, is widely prescribed for depressive disorders in China. Previous preclinical studies reported antidepressant-like effects only after weeks of repeated administration, mostly in male animals. Whether a single dose of CSS can produce rapid antidepressant-like effects in female animals, and through what mechanism, remains unclear. - Source: PubMed
Publication date: 2026/09/22
Wu LeiCheng WenqingXiang HaokeZhu YapingJiang YiranWei ZixuanShao MengqiTian LiyuanSun YanLu Chao - To investigate the molecular mechanisms by which calcium signaling pathways regulate the paclitaxel resistance and the survival of oral squamous cell carcinoma (OSCC) cells. - Source: PubMed
Wang JunfengChen HongliChen JiaoFeng YunYuan YaoZhang Ping - CaMKIIα functional knockout mouse (K42R) showed substantial changes in the entrainment pattern of running-wheel (RW) rhythm to a short (LD6:18) but not to a long (LD18:6) photoperiod. In wild type mice (WT), entrainment to LD6:18 occurred at the light-off phase, whereas in most K42R entrainment took place at the light-on phase. These differences are well explained by lengthening of circadian period (τ) and compromising the couplings between the activity-onset and -end in K42R. Indeed, τ in K42R and WT were longer and shorter than 24 h, respectively, under the following constant darkness (DD). Without RW τ was lengthened in WT, whereas it was shortened in K42R. Similarly, activity time was lengthened in WT but shortened in K42R. Importantly, τ without RW was not different between the two genotypes, which were confirmed in naive mice. Tau and α returned to the pre-removal levels after RW re-introduction in both WT and K42R. These results indicate that the changes of behavioral rhythm in K42R are due to a lack of feedback effects of RW activity, which are mediated by CaMKIIα-dependent mechanisms. A two-oscillator model assuming involvement of CaMKIIα in the coupling between them successfully simulated the effects of RW activity in K42R. - Source: PubMed
Publication date: 2026/09/02
Yoshikawa TomokoHonma Ken-IchiTokuda IsaoHonma Sato - β-Amyloid precursor protein (APP), an amyloid β-peptide (Aβ) generating protein, plays a central role in Alzheimer's disease (AD). Its intracellular trafficking and proteolytic processing are precisely regulated, but the mechanisms remain elusive. Here, we identified LAMTOR1 as a novel APP intracellular domain binding partner that sorts APP from early endosomes to late endosomes. Knockout of LAMTOR1 decreased APP trafficking to late endosomes, which promoted its amyloidogenic processing on early endosomes, resulting in increased Aβ. Conditional knockout of LAMTOR1 in Camk2a-expressing neurons impaired memory function in AD mouse model. Furthermore, the targeting of APP to late endosomes is mediated by adaptor protein complex 3 (AP-3). AP-3 bound to LAMTOR1, and its depletion caused accumulation of LAMTOR1 and APP within early endosomes, subsequently increasing Aβ. Thus, LAMTOR1 functions as a regulator of APP trafficking to late endosomes and may serve as a potential therapeutic target for AD. - Source: PubMed
Publication date: 2026/08/26
Zhou ChenqianCao WenyuanCong RixuShan ChunyanTian YongluXu SizhengFeng WeiranLi YuanjunWang XunTeng JunlinZheng PengliHuang NingChen Jianguo