Concentrated polyclonal antibodies TGFb3
- Known as:
- Concentrated pab antibodies TGFb3
- Catalog number:
- RP148-05
- Product Quantity:
- 0.5ml
- Category:
- -
- Supplier:
- Diagnostic Biosystems
- Gene target:
- Concentrated polyclonal antibodies TGFb3
Related genes to: Concentrated polyclonal antibodies TGFb3
- Gene:
- TGFB3 NIH gene
- Name:
- transforming growth factor beta 3
- Previous symbol:
- ARVD1, ARVD
- Synonyms:
- -
- Chromosome:
- 14q24
- Locus Type:
- gene with protein product
- Date approved:
- 1989-05-10
- Date modifiied:
- 2019-04-23
Related products to: Concentrated polyclonal antibodies TGFb3
Related articles to: Concentrated polyclonal antibodies TGFb3
- Transforming growth factor-β (TGF-β) genes play critical roles in immune regulation, but their functions in teleost fish, particularly TGF-β2 and TGF-β3, remain poorly understood. In this study, two TGF-β genes, namely ToTGF-β2 and ToTGF-β3 were identified in pufferfish, Takifugu obscurus. Bioinformatics analysis revealed that both genes possess conserved TGFb_propeptide and TGFB domains, with ToTGF-β2 additionally containing a low-complexity region. Tissue distribution analysis by RT-qPCR indicated that ToTGF-β2 showed the highest expression in the intestine, whereas ToTGF-β3 was most abundant in the head kidney. Following challenge with Vibrio parahaemolyticus or Vibrio harveyi, both genes were significantly upregulated in head kidney and spleen in a time-dependent manner, suggesting active participation in antibacterial immune responses. To further investigate their functional roles, in vivo RNA interference was performed. Knockdown of ToTGF-β2 significantly reduced the expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and impaired bacterial clearance, whereas knockdown of ToTGF-β3 markedly increased cytokine levels and enhanced bacterial elimination. These results demonstrate that ToTGF-β2 acts as a positive regulator of inflammation and host defense, while ToTGF-β3 functions as a negative regulator. This study provides novel insights into the functional diversification of TGF-β genes in fish antibacterial immunity. - Source: PubMed
Publication date: 2026/06/05
Liang FenfeiLiu KunZhou XiaoruiWang JinghanLi YufengSun YongqingLi ZhaoyangLiu XinXu YuweiZhang YihanZhang Guosong - Loeys-Dietz Syndrome (LDS) is a heritable connective tissue disorder characterized by widespread arterial tortuosity and aneurysmal disease. Although intracranial aneurysms occur frequently in LDS, it is unclear whether cervical arterial tortuosity is associated with intracranial aneurysm risk. - Source: PubMed
Publication date: 2026/05/23
Lee Jin VivianHuguenard Anna LConnor Michelle RBraverman Alan CDacey Ralph GOsbun Joshua W - Despite advances in tissue engineering, the clinical application of scaffolds for meniscal repair remains limited by implantation challenges and the difficulty of directing stem cells toward a stable meniscal phenotype. Here, we present an injectable hydrogel composed of silk fibroin (SF) and tannic acid (TA), codelivering transforming growth factor-β (TGF-β) and the transcription factor Mohawk (MKX). TA enhanced the β-sheet content of SF, yielding a hydrogel with superior rheological stability and shear-thinning behavior suitable for minimally invasive administration. The ST hydrogel provided sustained release of both bioactive factors over 35 days, with cumulative release below 16%. MKX attenuated TGF-β-induced hypertrophic differentiation of adipose-derived mesenchymal stem cells, promoting a fibrochondrocyte-like phenotype characterized by elevated expression of , , and aggrecan. Inflammatory stimulation assays demonstrated downregulation of osteoarthritis-related genes (e.g., , , and ) in the ST-TM group. In a rabbit meniscus tear model, ST hydrogels loaded with TGF-β and MKX significantly enhanced extracellular matrix deposition, promoted structural repair of the meniscus, and improved biomechanical properties at 10 weeks postimplantation. These findings support the potential of this dual-factor hydrogel system as a cell-free therapeutic strategy for functional meniscus regeneration, with potential relevance to the modulation of OA-related degeneration.Impact StatementThis study presents a novel injectable silk fibroin/tannic acid hydrogel codelivering transforming growth factor-β and Mohawk, which synergistically promotes fibrochondrocyte differentiation and inhibits hypertrophy of stem cells. Demonstrating injectable handling characteristics, controlled release, and efficacy, this cell-free platform offers a clinically translatable strategy for functional meniscus repair, with potential protective effects against osteoarthritis-related degeneration. - Source: PubMed
Publication date: 2026/05/23
Chen MingxueWang HaoZhao ZhijieLi ShenGuan MingjingLi Yangyang - Chronic wounds impose a serious healthcare burden with current options limited by efficacy, high cost, and risks of infection or scarring. Transforming growth factor3 (TGF-3) is attractive for regulating healing, limiting scarring, and promoting extracellular matrix remodeling, yet its translation ability impacted by rapid degradation and need for controlled local delivery. The eggshell membrane (ESM) is a biocompatible, collagen-rich, low-cost natural scaffold suitable for growth factor delivery. Although chicken ESM (CESM) is better studied, comparative assessment of duck (DESM) and ostrich (OESM) remains scarce. Here we systematically evaluate CESM, DESM, and OESM to establish their suitability for localized TGF-3 delivery. Using Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry, dynamic mechanical analysis, water contact angle measurement, and scanning electron microscopy, we show that all three membranes possess collagen-rich fibrous networks, high porosity, and thermal stability (> 135 °C). Hydrated membranes were hydrophilic, supporting a moist wound milieu. DESM resisted hydration-induced change, whereas OESM showed superior extensibility that may benefit dynamic wound regions.release revealed sustained TGF-3 delivery within 72 h across all membranes, with CESM and DESM providing more stable release than OESM. Cytotoxicity lactate dehydrogenase (LDH), viability (MTS), and live/dead assays indicated safety; dermal fibroblasts maintained ∼ 105% viability at 0.01-300 ng ml.chorioallantoic membrane (CAM) assays showed that TGF-3 increased vessel thickness, with DESM plus TGF-3 yielding the greatest effect, while branching responses were variable. This first comparative evaluation of CESM, DESM, and OESM as TGF-3 delivery platforms integrates materials characterization withandassays and supports ESM-based dressings for chronic wound care. - Source: PubMed
Publication date: 2026/05/26
Lu JunyiOyhanart Sharon RKnowles Jonathan CChau David Y S - Biophysical and biochemical cues in the local cellular microenvironment, including topography, matrix mechanics, and growth factors, significantly regulate stem cell fate. However, strategies for in vitro replicating such complex organized three-dimensional (3D) cellular microenvironments and modulating cell alignment and differentiation by these cues in cell-laden hydrogels are far less developed. This study introduces light-responsive collagen peptide hydrogels mixed with magnetic nanoparticles as physical crosslinkers, leading to in situ formation of an organized network of human bone marrow mesenchymal stem cells (hMSCs) under magnetic-driven anisotropy. Moreover, by simply tailoring the nanoparticle surface with dopamine methacrylamide, chemical nanoparticle crosslinkers with dual magnetic-light-responsiveness are developed to tune matrix mechanical dynamics without significantly changing hydrogel stiffness and components. The encapsulated hMSCs exhibit enhanced spreading, alignment, and differentiation into ligamentocytes/tenocytes in anisotropic hydrogels with faster mechanical dynamics and transforming growth factor beta-3, contributing to the synergistic effects of these biophysical and biochemical cues. These simple manufacturing and conditioning strategies, which directly incorporate stimuli-responsive nanoparticle crosslinkers into cell-laden hydrogels, show great potential in developing advanced 3D organized in vitro models to modulate stem cell organization and fate. - Source: PubMed
Publication date: 2026/05/14
Weng HongjuanChen WenHe LeiRademakers Timovan Rijt SabineDecarli Monize CBernaerts Katrien VMoroni Lorenzo