BHMT
- Known as:
- BHMT
- Catalog number:
- ENZ-292
- Product Quantity:
- 10µg
- Category:
- -
- Supplier:
- Prospecbio
- Gene target:
- BHMT
Related genes to: BHMT
- Gene:
- BHMT NIH gene
- Name:
- betaine--homocysteine S-methyltransferase
- Previous symbol:
- -
- Synonyms:
- BHMT1
- Chromosome:
- 5q14.1
- Locus Type:
- gene with protein product
- Date approved:
- 1997-06-09
- Date modifiied:
- 2014-11-19
Related products to: BHMT
Related articles to: BHMT
- Abnormally high levels of hypochlorite (ClO) can cause serious damage to environmental and biological systems, thereby highlighting the necessity for detectors capable of sensitive monitoring and quantification of ClO in these systems. In this study, we synthesized and evaluated a practical and portable fluorescent turn-off sensor, BHMT-Cl ((E)-2-(2-(3-(benzo[d]thiazol-2-yl)-2-hydroxy-5-methylbenzylidene)hydrazineyl)-N,N,N-trimethyl-2-oxoethan-1-aminium chloride), for the detection of ClO. BHMT-Cl exhibited selective and sensitive detection of ClO in pure water via a green fluorescence turn-off, with a critically low detection limit of 2.78 μM. The sensing mechanism was elucidated to involve the cleavage of the imine bond in BHMT-Cl, supported by H NMR titration, ESI-mass analysis, FT-IR and DFT calculations. BHMT-Cl was readily applied for the sensitive detection and quantification of ClO in diverse environmental water samples and demonstrated portability through smartphone-based analysis and test kits. In biological applications, BHMT-Cl enabled bioimaging of ClO in zebrafish as well as in fungal and plant systems, confirming its broad applicability. Overall, BHMT-Cl showed strong potential as a versatile tool for monitoring ClO in biological and environmental matrix. - Source: PubMed
Publication date: 2026/08/03
Lee AyinBatsaikhan OtgontsetsegLee Han-SeulKim Ki-TaeKim Cheal - Protein biomarkers are increasingly recognized as indicators of metabolic status, immune response, and reproductive performance in cattle. However, comparative data among different breeds commonly raised in Indonesia remain limited. - Source: PubMed
Publication date: 2026/08/04
Gustina SriHasbi HasbiAzis Ismah UlfiyahMaulana TulusHamsir Siti Nuraisya - : Dihydromyricetin (DHM) is a food-derived flavonoid enriched in vine tea and has been reported to possess antioxidant and metabolism-regulating properties. This study was designed to characterize the multi-level nutritional responses to dietary DHM supplementation, with emphasis on hepatic redox-inflammatory status, jejunal barrier-related phenotypes, cecal microbiota remodeling, hepatic metabolomic alterations, and homocysteine (Hcy) metabolism-related markers in mice. : Forty-eight healthy mice were assigned to a basal-diet control group or diets containing 50, 100, or 200 mg/kg DHM for 4 weeks. Growth performance, serum biochemistry, antioxidant parameters, hepatic antioxidant-related expression, hepatic inflammatory cytokines, jejunal morphology and tight junction proteins, cecal 16S rRNA profiles, hepatic metabolomics, and Hcy metabolism-related markers were assessed. : Dietary DHM improved serum and hepatic antioxidant status, as reflected by increased T-AOC and GSH-Px activity and decreased MDA concentrations ( < 0.05). DHM also modulated the hepatic cytokine profile, with decreased TNF-α concentration ( < 0.05) and increased IL-10 concentration ( < 0.01). DHM increased hepatic Nrf2 protein abundance, HO-1 protein abundance, and Gclc mRNA expression ( < 0.05). DHM also improved jejunal villus architecture, as indicated by increased villus height, decreased crypt depth, and an increased villus height-to-crypt depth ratio ( < 0.05). Jejunal Occludin and ZO-1 protein expression were increased in the DHM-treated groups ( < 0.05). Cecal microbiota analysis showed increased richness and diversity indices and altered microbial community structure. Hepatic metabolomics revealed changes involving vitamin B6 metabolism, purine metabolism, the pentose phosphate pathway, and α-linolenic acid metabolism. Serum Hcy levels decreased ( < 0.05), accompanied by increased hepatic BHMT and MTHFR protein abundance ( < 0.01). : Dietary DHM supplementation improved hepatic redox status and supported a less pro-inflammatory cytokine profile in mice, accompanied by enhanced jejunal barrier-related phenotypes, cecal microbiota remodeling, hepatic metabolic alterations, and Hcy metabolism-related responses. These findings provide a multi-level nutritional evaluation of DHM and suggest its potential relevance for supporting intestinal barrier integrity and hepatic metabolic homeostasis under basal physiological conditions. - Source: PubMed
Publication date: 2026/07/22
Liang WenjiaoTang LishiyuanFan JinghuiHuang RuiChen JiaxuanQian Lichun - Imbalance of maternal diets can impair offspring development and contribute to congenital anomalies of the kidney and urinary tract (CAKUT). Excess maternal methionine intake is known to impair fetal development. The placenta serves as a crucial interface for maternal-fetal exchange and may contribute to these adverse outcomes. We hypothesize that excess methionine intake during pregnancy in mice increases the incidence of CAKUT in offspring. Therefore, we established a mouse model of 10% (w/w) methionine intake during pregnancy from Embryonic Day 8.5 to11.5 (E8.5-E11.5) and assessed the kidney phenotype. Crucial kidney developmental pathways were analyzed by RT-qPCR and immunofluorescence at E11.5. Changes in methionine metabolism in maternal plasma and placental tissues at E11.5 were analyzed using liquid chromatography-tandem mass spectrometry, and placental RNA-seq was performed. Offspring from dams exposed to excess methionine exhibited increased incidence of CAKUT (relative risk = 4.15, 95% confidence interval: 1.40-12.34, P = .010), primarily presenting as hydronephrosis and/or duplex collecting system. E11.5 embryonic kidneys showed shorter common nephric duct and decreased activity of the Gdnf/Ret/p-Akt pathway. At E11.5, homocysteine was elevated in the placentas, together with decreased mRNA expression of key metabolic enzymes betaine-homocysteine S-methyltransferase (Bhmt) and cystathionine γ-lyase (Cth) in the placentas. Concurrently, RNA-seq of E11.5 placentas revealed alterations in immune-inflammatory and vascular-related pathways. Overall, excessive maternal methionine intake in mice increased the incidence of CAKUT in offspring, which may be associated with altered activity of the Gdnf/Ret/PI3K/p-Akt pathway in embryonic kidneys as well as elevated placental homocysteine. - Source: PubMed
Publication date: 2026/07/06
Ye NingliYu MinghuiJu HaixinJiang YuanyanZhang YumengSun LeiWu XiaohuiChen JingXu HongShen Qian - Grass carp () is a major freshwater aquaculture species in China, but its growth is limited under intensive high-density farming. This study aimed to investigate the characteristics of plasma exosomes associated with distinct growth performance by isolating and characterizing exosomes from fast- and slow-growing grass carp after nine months of culture. Exosomes showed typical morphology and expressed characteristic markers (CD63, CD81, TSG101). Small RNA sequencing identified 3325 miRNAs, with 177 highly abundant miRNAs differentially expressed: immune-related miRNAs were upregulated, while development-inhibitory miRNAs were downregulated in fast-growing fish. Target gene enrichment highlighted pathways in neural and skeletal development and amino acid metabolism. Integrative analysis across tissues revealed 26 miRNAs with coordinated expression patterns between plasma exosomes and brain, liver, or muscle, validated by qPCR. DIA proteomics quantified 4203 proteins, identifying 843 differentially enriched proteins linked to immune response, energy metabolism, and endoplasmic reticulum stress. Notably, TYMP was upregulated in muscle and exosomes, while several proteins (e.g., GYG2, BHMT) showed coordinated downregulation across tissues and exosomes in large fish. These results provide comprehensive evidence of exosome-mediated cross-tissue communication in teleosts and suggest a potential role for plasma exosomal miRNAs and proteins as non-invasive biomarkers correlated with growth status in aquaculture. - Source: PubMed
Publication date: 2026/07/06
Zhu TengfeiZheng ZhipengChen HaoYu YingyingGuo HuayangLiu BaosuoZhu KechengZhang NanXian LinZheng ShuhuiLiu YangChen SonglinZhang Dianchang