TACS Blue Label
- Known as:
- TACS Blue Label
- Catalog number:
- 4800-30-11
- Product Quantity:
- 3 ml
- Category:
- -
- Supplier:
- Trevigen
- Gene target:
- TACS Blue Label
Related genes to: TACS Blue Label
- Gene:
- EPCAM NIH gene
- Name:
- epithelial cell adhesion molecule
- Previous symbol:
- M4S1, MIC18, TACSTD1
- Synonyms:
- Ly74, TROP1, GA733-2, EGP34, EGP40, EGP-2, KSA, CD326, Ep-CAM, HEA125, KS1/4, MK-1, MH99, MOC31, 323/A3, 17-1A, TACST-1, CO-17A, ESA
- Chromosome:
- 2p21
- Locus Type:
- gene with protein product
- Date approved:
- 1995-10-02
- Date modifiied:
- 2019-04-23
Related products to: TACS Blue Label
Related articles to: TACS Blue Label
- Penile squamous cell carcinoma (PSCC) can affect men of any age, but we know little about the differences between non-old and old patients with PSCC, which severely limits the precise diagnosis and treatment of PSCC. - Source: PubMed
Publication date: 2026/06/06
Cao JianDu LinQin ZailongMa AiweiBo HaoZhao XuehengLuo YanweiOu ChunlinLiu ZhizhongXie YuZhang WancongTang ShijieTian YuYuan JunbinGong ZhaojianGuo Jie - Extracellular vesicles (EV) serve as critical mediators in physiological and pathological processes, holding great promise for cancer diagnosis, real-time monitoring, and prognostic applications. However, their small size and low density present considerable challenges in achieving efficient, specific, and mild isolation, which currently hinders widespread clinical translation. Here, we report a proximity-induced pH-responsive DNA switch (PPS) that enables reversible EV capture via Hoogsteen triplex formation. Specifically, dual-aptamer probes targeting EpCAM and CD63 on EV exploit membrane fluidity to form proximity-induced duplex helix, which assemble into Hoogsteen triplex helix with magnetic bead-conjugated strands at mildly acidic conditions (pH6.5) for EV capture. Crucially, simply adjusting the pH to neutral (pH7.4) triggers triplex dissociation, releasing intact EV without using chemical denaturants and preserving vesicle integrity for high-purity isolation. Under optimized conditions, this method achieves 79.4% and 72.6% EV purification efficiency in PBS and complex biological matrices, respectively, within 1 h. Furthermore, its modular aptamer design enables rapid adaptation to diverse EV subpopulations through simple probe substitution, without modifying the core framework, thereby reducing cost and complexity for broad applications. Meanwhile, due to the gentle capture-release process, the structural integrity and bioactivity of EV are well preserved, as demonstrated by wound-healing and cellular uptake assays. These advantageous features─rapid processing, high specificity, mild operation, and preservation of EV activity─indicate that the PPS strategy is a robust, nondestructive method for EV isolation. It thus holds significant potential for further application in diverse EV-related research fields such as disease diagnosis and drug delivery. - Source: PubMed
Publication date: 2026/06/06
Wang LeiShi YantingCao ChenyuShen YuqianLiu Dong - Epithelial cell adhesion molecule ()-associated Lynch syndrome arises from deletions at the 3'-end of that disrupt transcriptional termination, generate read-through transcripts and cause epigenetic silencing of MSH2 in EPCAM-expressing tissues. However, the clinical significance of deletions confined to the intergenic region remains uncertain without in-depth investigation.We investigated a family with a strong history of Lynch syndrome-related cancers in whom diagnostic testing by short-read sequencing identified a heterozygous deletion spanning the intergenic region that was initially classified as a variant of uncertain significance. The variant was further characterised using long-read Oxford Nanopore sequencing with adaptive sampling and methylation profiling.Long-read sequencing defined precise breakpoints, and tumour analysis demonstrated promoter hypermethylation with complete loss of MSH2 protein expression in the absence of germline promoter methylation. The molecular phenotype closely mirrored the recognised mechanism for 3'-end deletions, whereby aberrant transcription interferes with promoter regulation in a tissue-specific manner.These findings support reclassification of this variant to likely pathogenic and establish a diagnosis of -associated Lynch syndrome. This report provides the first evidence that intergenic deletions are associated with epimutations and highlights the diagnostic utility of long-read sequencing for noncoding structural variants. - Source: PubMed
Publication date: 2026/06/05
Steffens Reinhardt LuizaCoster AlexanderBurnard Sean MRomanis Caitlin SZiolkowski AndrewPecenpetelovska GordanaMathe AndreaHedley AmeliaMaltby Vicki ELechner-Scott JeannetteAshton KatieAvery-Kiejda Kelly AScott Rodney J - Nectin‑4, a cell adhesion molecule and emerging therapeutic target in several malignancies, has not been fully characterized in renal cell carcinoma (RCC). This study aimed to define the clinical significance, biological function, and therapeutic implications of Nectin‑4 across RCC subtypes. Immunohistochemistry of 273 primary RCC samples revealed that the expression of Nectin‑4, although present in a minority of clear cell RCC (ccRCC) cases, was significantly enriched in nonclear cell RCC (nccRCC). Nectin‑4 positivity was correlated with adverse pathological features, including higher pT stage, tumor grade, and venous invasion. In ccRCC, the expression of Nectin‑4 was associated with inferior overall survival, consistent with the findings from a public dataset analysis. In vitro experiments demonstrated that Nectin‑4 overexpression enhanced the proliferation and invasion of multiple RCC cell lines, supporting a tumor‑promoting role. Transcriptomic analyses across TCGA‑KIRC, TCGA‑KIRP, JAVELIN Renal 101, and CheckMate‑025 cohorts showed that the high expression of NECTIN4 was strongly associated with epithelial-mesenchymal transition, inflammatory signaling, sarcomatoid features, and genomic dedifferentiation. Despite these aggressive biological features, the expression of NECTIN4 was not correlated with immune infiltration markers and did not predict the clinical benefit of immune checkpoint inhibitors. These findings suggest that Nectin‑4 is a marker of tumor aggressiveness and dedifferentiation, rather than an immunotherapy biomarker. Given its relatively high expression in nccRCC and its functional contribution to malignant behavior, Nectin‑4-directed antibody-drug conjugates may represent a promising therapeutic option for this underserved population and warrant further investigation. - Source: PubMed
Publication date: 2026/06/05
Hatayama TomoyaSekino YoheiBabasaki TakashiSentani KazuhiroNakahara HikaruShikuma HiroyukiIwane KyosukeYukihiro KazumaKohada YukiNaito MikiMiyamoto ShunsukeKobatake KoheiKitano HiroyukiGoto KeisukeHieda KeisukeHinoi TakaoHinata Nobuyuki - Direct lineage reprogramming offers a highly promising non-genetic pathway for generating functional somatic cells. In this study, a single small molecule inhibitor of the BMP pathway, LDN193189, was able to directly reprogram goat ear fibroblasts into Chemically induced Mammary Epithelial Cells (CiMECs) in vitro within 8 days. The generated CiMECs displayed typical epithelial morphology, expressed key mammary epithelial markers (including KRT8/14/18/19, CD49f, and EpCAM), and exhibited potent secretory functions: they could form lipid droplets and produce milk proteins such as lactoferrin (LTF) and αS2-casein (αS2-CSN). Single-cell RNA sequencing revealed a stepwise reprogramming trajectory: starting from mesenchymal-epithelial transition (MET), through ectoderm-like and epidermal-like intermediate states to differentiate into basal and lumenal mammary epithelial cells, which involves multiple signaling pathways related to mammary gland development, such as TGF-β, MAPK, and PI3K-Akt. Functionally, CiMECs could self-assemble in vitro to form three-dimensional mammary-like organs. In addition, in vivo transplantation experiments in nude mice showed that these CiMECs survived and contributed to ductal- and basal-like tissue structures, confirming their in vivo regenerative potential. In conclusion, this study establishes a simple somatic cell-based platform with a well-defined chemical composition to efficiently generate functional mammary epithelial cells, providing an important model for mammary developmental studies and opening up promising strategies for regenerative medicine and bioreactor engineering applications. - Source: PubMed
Liu QuanhuiZhang DandanWang GuodongLei ZhigangDeng ShanXiao LiangguiQin MengqinHuang Ben