UBE2D1 Antibody
- Known as:
- UBE2D1 Antibody
- Catalog number:
- ENZ-007321-M01
- Product Quantity:
- 0.1mg
- Category:
- Antibodies
- Supplier:
- Zyagen
- Gene target:
- UBE2D1 Antibody
Related genes to: UBE2D1 Antibody
- Gene:
- UBE2D1 NIH gene
- Name:
- ubiquitin conjugating enzyme E2 D1
- Previous symbol:
- SFT
- Synonyms:
- UbcH5A, UBCH5, UBC4/5, E2(17)KB1
- Chromosome:
- 10q21.1
- Locus Type:
- gene with protein product
- Date approved:
- 1997-03-21
- Date modifiied:
- 2016-03-14
Related products to: UBE2D1 Antibody
Related articles to: UBE2D1 Antibody
- Type 2 diabetes mellitus (T2DM) and sarcopenia demonstrate a significant comorbidity, particularly in the elderly, yet the molecular mechanisms linking them, especially through oxidative stress, remain incompletely understood. This study aimed to identify oxidative stress-related hub genes involved in T2DM-associated sarcopenia (T2DS) by integrating single-cell RNA sequencing (scRNA-seq) and bulk RNA-seq data with machine learning. We analyzed scRNA-seq datasets (GSE244515, GSE268953) to characterize cellular heterogeneity and bulk RNA-seq datasets (GSE202295, GSE226151) for differential expression. Cell type annotation revealed key involvement of neuromuscular junctions and myofibers. Functional enrichment analyses highlighted pathways like the proteasome, TNF signaling, and ubiquitin-mediated proteolysis. From an initial set of oxidative stress-related genes, a comprehensive machine learning framework comprising 127 algorithm combinations was employed. The Lasso+Stepglm[both] model identified 12 candidate genes. Subsequent Protein-Protein Interaction (PPI) network analysis refined this to seven core hub genes: TNFRSF1B, PSMA2, UBE2D1, UBE2N, HSP90AA1, RAD23A, and DNAJB1. These genes are functionally interconnected, primarily implicating TNFRSF1B-mediated inflammatory signaling that activates the ubiquitin-proteasome system, leading to enhanced protein degradation-a key pathway in muscle atrophy. ROC curve analysis confirmed the strong diagnostic value of these hub genes across training, test, and external validation sets. Our findings systematically reveal novel oxidative stress-related hub genes and mechanisms in T2DS, providing potential biomarkers and therapeutic targets for this debilitating condition. - Source: PubMed
Publication date: 2026/07/07
Zhu GuangwenZou KaiLiang YiXie LitingChen Qiu - Here, we present a protocol for the recombinant production of DTX3L and USP28, along with in vitro and in vivo procedures for examining their regulatory functions. We describe steps for reconstituting DTX3L-dependent ubiquitination in vitro with purified UBA1, UbE2D1, ubiquitin, and DTX3L and assessing USP28-mediated deubiquitination. We then detail procedures for the in vivo detection of ubiquitin chain linkage-type through co-immunoprecipitation and Western blotting. This adaptable approach enables analysis of regulatory crosstalk between E3 ligases and deubiquitinases within the ubiquitin system. For complete details on the use and execution of this protocol, please refer to Mennerich et al.. - Source: PubMed
Publication date: 2026/07/02
Mennerich DanielaVela-Rodriguez CarlosLehtiö LariKietzmann Thomas - [This corrects the article on p. 2948 in vol. 13, PMID: 37560007.]. - Source: PubMed
Publication date: 2026/05/25
Guan Xiao-QingYuan Xiao-NingFeng Kai-XiangShao You-ChengLiu QinYang Ze-LinChen Yan-YanDeng JinHu Mei-ShunLi JunTian Yi-HaoChu Meng-FeiZhang Jing-WeiWei Lei - Systemic juvenile idiopathic arthritis (sJIA) is an autoinflammatory disorder characterized by systemic immune dysregulation, yet reliable biomarkers to predict its unpredictable disease course are lacking. Identifying immune cell subsets and molecular drivers of disease progression is essential for improving prognosis and developing targeted therapies. Here, we performed comprehensive immunophenotypic profiling of PBMCs from sJIA patients across five clinical centers. We identified an unrecognized CD14CXCL10 monocyte subset in sJIA distinguished by a unique transcriptomic signature enriched in immune regulatory genes. Deconvolution analysis with longitudinal follow-up in the Chongqing cohort revealed a previously unrecognized CD14CXCL10 monocyte subset that was markedly expanded during active sJIA and diminished during remission, correlating strongly with disease activity. Flow cytometry confirmed its dynamic changes, and inflammatory stimulation promoted the differentiation of monocytes into the CXCL10 phenotype. To validate these observations , we used Ube2d1 knockout mice, which exhibit impaired CXCL10 induction and attenuated arthritis severity, highlighting the pivotal role of Ube2d1 in driving this inflammatory program. Furthermore, a cross-disease single-cell reference atlas demonstrated that this monocyte subset displayed a distinct expression profile in sJIA compared with other JIA subtypes and inflammation-related diseases. Collectively, our findings indicate that UBE2D1-driven CD14CXCL10 monocytes are central to sJIA pathogenesis and may represent both a biomarker and a therapeutic target for disease monitoring and intervention. - Source: PubMed
Publication date: 2025/11/19
Luo QiangYang JunYu HaiguoHao HanWu XinglinLuo XiwenMa MingshengYang XiZhang ZhiyongAn YunfeiZhao XiaodongSong HongmeiTang Xuemei - Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy with poor clinical outcomes. Ubiquitin-conjugating enzyme E2 D1 (UBE2D1), a key component of the ubiquitination machinery, has been implicated in tumor progression in several cancers; however, its relevance in HNSCC remains unclear. This study aimed to investigate UBE2D1 expression in HNSCC, evaluate its prognostic significance while acknowledging disease heterogeneity, including HPV and TP53 status, and explore its potential association with response to anti-PD-1 immunotherapy. - Source: PubMed
Publication date: 2026/02/14
Prasad PrathibhaArumugam Paramasivam