Sheep Versican ELISA, VCAN
- Known as:
- Sheep Versican Enzyme-linked immunosorbent assay test, VCAN
- Catalog number:
- E14V0050
- Category:
- -
- Supplier:
- Blue Gene Biotech
- Gene target:
- Sheep Versican ELISA VCAN
Related genes to: Sheep Versican ELISA, VCAN
- Gene:
- VCAN NIH gene
- Name:
- versican
- Previous symbol:
- CSPG2
- Synonyms:
- PG-M
- Chromosome:
- 5q14.2-q14.3
- Locus Type:
- gene with protein product
- Date approved:
- 1991-07-18
- Date modifiied:
- 2018-02-13
Related products to: Sheep Versican ELISA, VCAN
Related articles to: Sheep Versican ELISA, VCAN
- The extracellular matrix (ECM) is a major component of the tumor microenvironment (TME) and plays an important role in cervical cancer progression. Versican, encoded by the VCAN gene, is a member of the aggrecan/proteoglycan family and has been reported as a marker of poor prognosis in cervical cancer. However, the versican-producing cells in cervical cancer tissue are not clearly identified. To identify the versican-producing cells in cervical cancer tissue, single-cell RNA sequencing (scRNA-seq) data of cervical cancer tissue are re-analyzed, and we find that versican is highly expressed in monocytes and fibroblasts. Immunohistochemical staining of cervical cancer and adjacent normal tissues showed that versican was predominantly expressed in non-parenchymal cells. Multiplex immunofluorescence staining further identified versican-expressing macrophages in cervical cancer tissues. We subsequently collected peripheral blood mononuclear cells (PBMCs) from a patient with cervical cancer before and after radiotherapy and performed scRNA-seq to investigate the highly variable genes in PBMCs. Notably, VCAN was identified as a monocyte-enriched gene and showed relatively high expression in the patient's circulating monocytes. We further investigated the effect of Versican core protein (VCAN-V3) on macrophages. The results suggest that VCAN-V3 predominantly suppresses M1-associated gene expression and induces a partial shift toward an M2-like transcriptional phenotype in RAW264.7 cells. In conclusion, our study demonstrates that stromal VCAN is upregulated in cervical cancer tissue. VCAN-V3 may influence the tumor microenvironment by modulating macrophage polarization-associated responses. - Source: PubMed
Publication date: 2026/08/28
Liu ShijiaYang TongguoChu AlanSun ChenChai TingLi MengxiLiu ZongwenZhang Mengfan - Endometriosis is a multifaceted disease, causing debilitating pelvic pain in some patients, while being completely asymptomatic in others. The pathophysiology of endometriosis pain is not well understood and poorly correlated to stage. We hypothesized that this clinical heterogeneity may be explained by examining differences in gene expression. We performed RNA sequencing of 27 formalin-fixed, paraffin-embedded peritoneal biopsies from 9 symptomatic (Sx) and 10 asymptomatic (ASx) subjects. 890 genes were differentially expressed between Sx and ASx samples. Of these, the most significant including genes involved inflammatory signaling (IL16, IL17RA, JAK3, SMPD3, RELT), cell adhesion (OLFML1, CDON, VCAN), and neuromodulation (SEMA6D, ADRA2C, SLC7A5). Gene Set Enrichment Analysis identified functional enrichment in 22 gene ontology (GO) pathways, of which 7 represented immunologic pathways. Weighted Gene Correlation Network Analysis (WGCNA) identified 11 co-expression modules significantly correlated with symptomaticity, including one module strongly enriched for immunologic/inflammatory functions. Of all molecules identified as associated with pain, IL16 expression also correlated with symptom severity when cases were stratified into mild vs. severe pain based on clinical criteria. These findings suggest that endometriotic lesions of symptomatic subjects are characterized by distinct molecular signatures, including altered expression of inflammatory pathways, highlighting potential mechanisms underlying symptom variability and identifying candidate pathways for future therapeutic interventions. - Source: PubMed
Publication date: 2026/09/10
Mamillapalli RamanaiahLi Howard JApelian ShantKumar MonishWang Sarah FPondugula NishitaCampos Gabriela de QueirozSayeed SumaiyaCho YongheeTaylor Hugh S - The cellular composition and disease susceptibilities of the distinct zones of the human prostate remain incompletely understood. Benign prostatic hyperplasia (BPH) is a common condition that causes widespread morbidity and is nearly exclusively localized to the transition zone (TZ). Through extensive single-cell RNA sequencing (scRNA-seq) of benign regions from prostatectomy specimens, we identified a basal cell population expressing WIF1, VCAN, and NRG1, among other genes, that was significantly enriched in the TZ. Analysis of previously published scRNA-seq datasets further confirmed that WIF1 basal cells were significantly enriched in BPH compared with normal prostate. Pathway and cell-cell communication analyses revealed that this basal subtype is associated with programs related to cell proliferation, epithelial-mesenchymal transition, immune regulation, angiogenesis, and hormone response. Together, the molecular signature, zonal distribution, and pathway enrichment suggest that TZ-enriched WIF1 basal cells may contribute to BPH pathogenesis by promoting epithelial and stromal remodeling. © 2026 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. - Source: PubMed
Publication date: 2026/09/10
Wang RulinZheng QizhiGraham Mindy KVaghasia AjayLiu JianyongGregg JordanJones TracyGupta AnujCastagna NicoleZhang YanSchuebel KornelMeyers JenniferSkaist AlyzaHoyle DixieKung JasmineHicks JessicaMishra AlokYang YuhanNelson William GDe Marzo Angelo MYegnasubramanian Srinivasan - Poor pathological response to neoadjuvant immunotherapy combined with chemotherapy (nICT) limits long-term survival in resectable lung cancer. How to improve response rates and the regulatory mechanism of tumor inflammatory signaling in nICT efficacy remain unclear. - Source: PubMed
Publication date: 2026/09/02
Chen XinxinZhang SiangAi XuefengFan YongfeiChen Wenjie - Nephrotic syndrome (NS) carries a high and heterogeneous risk of venous thromboembolism (VTE). Hypercoagulability has been considered as key factors, but in-depth molecular mechanisms remain unrevealed. - Source: PubMed
Publication date: 2026/07/14
Liu XinMa JingLyu YanshuangZhang XiaomengSong WanluYang PeiranZhai ZhenguoFan GuohuiWang Dingyi