Porcine Matrix extracellular phosphoglycoprotein ELISA, MEPE
- Known as:
- Porcine Matrix extracellular phosphoglycoprotein Enzyme-linked immunosorbent assay test, MEPE
- Catalog number:
- E07M0259
- Category:
- -
- Supplier:
- Blue Gene Biotech
- Gene target:
- Porcine Matrix extracellular phosphoglycoprotein ELISA MEPE
Related genes to: Porcine Matrix extracellular phosphoglycoprotein ELISA, MEPE
- Gene:
- MEPE NIH gene
- Name:
- matrix extracellular phosphoglycoprotein
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 4q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2000-09-19
- Date modifiied:
- 2016-10-05
Related products to: Porcine Matrix extracellular phosphoglycoprotein ELISA, MEPE
Related articles to: Porcine Matrix extracellular phosphoglycoprotein ELISA, MEPE
- Sleep disorders present major public health issues because of their associations with chronic diseases. Despite extensive research efforts, the underlying biological mechanisms remain inadequately understood. We performed bidirectional two-sample Mendelian randomization (MR) to investigate relationships between sleep disorders and common brain diseases, followed by summary-data-based Mendelian randomization (SMR), heterogeneity in dependent instruments (HEIDI), and colocalization analyses integrating protein quantitative trait locus (pQTL) and expression quantitative trait locus (eQTL) data to prioritize genetically supported candidate genes. Compound-gene associations were subsequently evaluated, and selected clinically used compounds underwent exploratory molecular docking and molecular dynamics simulation. Two-step MR mediation analyses and MR phenome-wide association study (MR-PheWAS) were used to investigate potential mediating traits and broader phenotypic associations. Multi-omic genetic analyses prioritized six candidate genes-BCAN, HDGF, MEPE, PAM, PRR4, and IGLON5-with BCAN, HDGF, and MEPE showing the strongest genetic support. Compound prioritization retained four clinically used drugs associated with the candidate genes for structure-based assessment. Exploratory molecular docking yielded the most favorable predicted Vina score for the BCAN-danazol pair, and molecular dynamics simulation showed persistent local BCAN-danazol contacts despite substantial global conformational rearrangement of BCAN during the trajectory. Mediation analyses identified several risk factors, cerebrospinal fluid metabolites, and structural connectivity measures showing statistical evidence consistent with partial mediation of selected gene/protein-sleep disorder associations. MR-PheWAS further characterized broader phenotypic associations of the genetically supported genes. Integrating genetic and multi-omic evidence identified candidate molecular targets and compound-gene associations relevant to sleep disorders. These findings provide hypotheses for subsequent functional and pharmacological validation. - Source: PubMed
Publication date: 2026/09/19
Wang ZiyuanZhang MiaoLiu CongZhang YiyinCheng RongZhang SuliYang BinWang LiGuo JunhongWang Xiaohui - Under matrix isolation conditions, weakly bound complexes can be stabilized and even higher energy species can be kinetically trapped and subsequently characterized spectroscopically. Halogen bonded and π-hole complexes of iodotrifluoroethylene and different tertiary amines are identified in pH2 matrices. For ITFE-trimethylamine, the CC stretching mode of ITFE is found to show a characteristic red-shift associated with σ-hole (halogen bonding) interactions with either N or p-systems as acceptors. In contrast, a blue-shift is characteristic of a π-hole interaction with either donor moiety. We demonstrate the transferability of these findings to other bulkier amines by studying complexes of ITFE with α,,-trimethyl benzyl amine (TMBA) and -methyl-,-bis(α-phenylethyl)amine (MePEA), for which only C-I⋯N and C-I⋯π complexes could be identified. The origin of the characteristic shifts of the CC stretching mode is discussed. Furthermore, it is shown that the matrix environment plays a key role in the complex formation process, that is, it blocks π-surfaces for secondary interactions after formation of stronger σ-hole contacts. - Source: PubMed
Publication date: 2026/09/18
Feßner MalteBeyer FrederikeKreienborg Nora MMerten Christian - Human phosphorylated acidic serine- and aspartate-rich motif (pASARM), derived from matrix extracellular phosphoglycoprotein, exhibits anti-calcification activity and induces hypophosphatemia by inhibiting phosphate uptake in the intestine and kidneys. pASARM is considered a potential therapeutic candidate for hyperphosphatemia and ectopic calcification in chronic kidney disease-mineral and bone disorder owing to its inhibitory effects on phosphate uptake and mineralization. However, developing effective monoclonal antibodies is challenging because of the high amino acid sequence homology among mammalian species and the low immunogenicity of pASARM in mice and rabbits. In contrast, chickens have attracted attention as an alternative immune animal model because of their greater phylogenetic distance from humans. In this study, we aimed to generate anti-ASARM mAbs using chicken-derived phage display technology. First, a highly diverse library of single-chain variable fragment (scFv) antibodies was constructed from chickens immunized with ASARM. Second, biopanning and cloning were performed using ASARM to isolate highly reactive scFv antibodies. Third, the selected scFv antibodies were converted into chicken-human chimeric anti-ASARM IgG (anti-ASARM IgG) for future effector-function applications. An enzyme-linked immunosorbent assay demonstrated that the two anti-ASARM IgG antibodies possessed high reactivity toward ASARM. Immunohistochemical analyses showed that one antibody successfully detected ASARM localization in the femoral tissues of 8-week-old male mice and age-matched male rats. The anti-ASARM IgG generated in this study exhibited low non-specificity and high reproducibility, making them potentially valuable tools for basic research involving ASARM. - Source: PubMed
Publication date: 2026/08/05
Kawanabe YodaiHasegawa TomokaYamamoto TomomayaNishimichi NorihisaYoshiko YujiWatanabe TenkaiMatsuzaki MeiEzaki RyoHoriuchi Hiroyuki - Graphitic carbon nitride nanoparticles (g-C3N4) are a promising material in wound healing; however, their effects and potential synergy with PRP (platelet-rich plasma) remain unexplored. This study evaluates the single and synergistic effects of PRP injection and g-C3N4 nanoparticle ointment on wound healing in dogs. Six full-thick skin wounds per group (a total of 6 groups) were surgically created on the chest region of six adult mongrel dogs, with each dog having three bilateral chest wounds. Wounds were treated with either normal saline, lanolin dressings, PRP infiltration, PRP infiltration combined with lanolin dressings, g-C3N4 NPs ointment, or PRP infiltration with g-C3N4 NPs application. Healing progression was monitored, with the evaluation of epithelialization and wound contraction. Total antioxidant capacity (TAC), malondialdehyde (MDA), and PDGFβ concentration were measured in wound fluid. Matrix extracellular phosphoglycoprotien (MEPE) and transforming growth factor beta (TGF-β) gene expressions were assessed in skin biopsies at 0, 5, 10 and 20 days. Wound histopathology and tumor necrosis factor alpha (TNF-α) immunohistochemistry (IHC) were performed. - Source: PubMed
Publication date: 2026/07/23
Wafy Mona NHassan Elham ASaeed SamarKhattab Marwa SAbuBakr Huda OAbu-Seida Ashraf M - Flooding compromises the microbiological quality of domestic and recreational water sources, increasing exposure to waterborne and opportunistic pathogens in affected communities. Following widespread flooding triggered by the September 2023 controlled spillage of the Akosombo and Kpong dams in Ghana, which led to massive flooding, we assessed the microbial quality of water bodies in communities downstream of the Volta River and associated public and ecosystem health implications. In July 2024, water was collected from multiple source types across four downstream townships (Asutsuare, Aveyime, Battor, and Mepe), including river sections, ponds, puddles, wells, boreholes, and canal-associated sites. Samples were filtered, microbial DNA was extracted, and bacterial community composition was profiled using 16S rRNA gene sequencing, with downstream bioinformatics analyses used to characterize microbial diversity, assess spatial variation across sites, and stratify detected taxa according to ecological origin and public health risk. Sequence data obtained from 17 out of 22 samples revealed communities dominated by Proteobacteria with frequent co-occurrence of Bacteroidetes and Firmicutes. Across all sites, 56 microbial taxa and 118 microorganisms were detected. Community composition varied by location and water source type, with bank and mid-river sites generally exhibiting higher richness than puddles and a canal drain. Thirteen samples contained site-specific taxa, indicating marked spatial heterogeneity within and between communities. Microorganisms of public health relevance were frequently detected, including enteric-associated bacteria such as Escherichia coli, Salmonella enterica, Shigella species, and Cronobacter sakazakii, as well as opportunistic and healthcare-associated bacteria including Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. Higher-risk organisms were most widely distributed in Mepe and Battor, including in groundwater-associated sources. These findings provide molecular evidence of persistent, spatially heterogeneous microbial hazards months after flooding, underscoring continued exposure risks in downstream communities and the need for targeted water safety interventions and risk-informed public health responses. - Source: PubMed
Publication date: 2026/07/02
Kpeli Grace SemabiaAgyirey-Kwakye PrinceLivingstone Counseller NutifafaCudjoe Lillian TeyeDotse Emmanuel EdemNyarko EbenezerZar EbenezerNyarko-Otu ObedAgbogli Hubert KwameGlilekpeh GodwinKorankye SolomonEssandoh PriscillaKabotso Daniel Elorm