Guinea pig Matrix extracellular phosphoglycoprotein ELISA, MEPE
- Known as:
- Guinea pig Matrix extracellular phosphoglycoprotein Enzyme-linked immunosorbent assay test, MEPE
- Catalog number:
- E05M0259
- Category:
- -
- Supplier:
- Blue Gene Biotech
- Gene target:
- Guinea pig Matrix extracellular phosphoglycoprotein ELISA MEPE
Related genes to: Guinea pig Matrix extracellular phosphoglycoprotein ELISA, MEPE
- Gene:
- MEPE NIH gene
- Name:
- matrix extracellular phosphoglycoprotein
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 4q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2000-09-19
- Date modifiied:
- 2016-10-05
Related products to: Guinea pig Matrix extracellular phosphoglycoprotein ELISA, MEPE
Related articles to: Guinea pig Matrix extracellular phosphoglycoprotein ELISA, MEPE
- Human phosphorylated acidic serine- and aspartate-rich motif (pASARM), derived from matrix extracellular phosphoglycoprotein, exhibits anti-calcification activity and induces hypophosphatemia by inhibiting phosphate uptake in the intestine and kidneys. pASARM is considered a potential therapeutic candidate for hyperphosphatemia and ectopic calcification in chronic kidney disease-mineral and bone disorder owing to its inhibitory effects on phosphate uptake and mineralization. However, developing effective monoclonal antibodies is challenging because of the high amino acid sequence homology among mammalian species and the low immunogenicity of pASARM in mice and rabbits. In contrast, chickens have attracted attention as an alternative immune animal model because of their greater phylogenetic distance from humans. In this study, we aimed to generate anti-ASARM mAbs using chicken-derived phage display technology. First, a highly diverse library of single-chain variable fragment (scFv) antibodies was constructed from chickens immunized with ASARM. Second, biopanning and cloning were performed using ASARM to isolate highly reactive scFv antibodies. Third, the selected scFv antibodies were converted into chicken-human chimeric anti-ASARM IgG (anti-ASARM IgG) for future effector-function applications. An enzyme-linked immunosorbent assay demonstrated that the two anti-ASARM IgG antibodies possessed high reactivity toward ASARM. Immunohistochemical analyses showed that one antibody successfully detected ASARM localization in the femoral tissues of 8-week-old male mice and age-matched male rats. The anti-ASARM IgG generated in this study exhibited low non-specificity and high reproducibility, making them potentially valuable tools for basic research involving ASARM. - Source: PubMed
Publication date: 2026/08/05
Kawanabe YodaiHasegawa TomokaYamamoto TomomayaNishimichi NorihisaYoshiko YujiWatanabe TenkaiMatsuzaki MeiEzaki RyoHoriuchi Hiroyuki - Graphitic carbon nitride nanoparticles (g-C3N4) are a promising material in wound healing; however, their effects and potential synergy with PRP (platelet-rich plasma) remain unexplored. This study evaluates the single and synergistic effects of PRP injection and g-C3N4 nanoparticle ointment on wound healing in dogs. Six full-thick skin wounds per group (a total of 6 groups) were surgically created on the chest region of six adult mongrel dogs, with each dog having three bilateral chest wounds. Wounds were treated with either normal saline, lanolin dressings, PRP infiltration, PRP infiltration combined with lanolin dressings, g-C3N4 NPs ointment, or PRP infiltration with g-C3N4 NPs application. Healing progression was monitored, with the evaluation of epithelialization and wound contraction. Total antioxidant capacity (TAC), malondialdehyde (MDA), and PDGFβ concentration were measured in wound fluid. Matrix extracellular phosphoglycoprotien (MEPE) and transforming growth factor beta (TGF-β) gene expressions were assessed in skin biopsies at 0, 5, 10 and 20 days. Wound histopathology and tumor necrosis factor alpha (TNF-α) immunohistochemistry (IHC) were performed. - Source: PubMed
Publication date: 2026/07/23
Wafy Mona NHassan Elham ASaeed SamarKhattab Marwa SAbuBakr Huda OAbu-Seida Ashraf M - Flooding compromises the microbiological quality of domestic and recreational water sources, increasing exposure to waterborne and opportunistic pathogens in affected communities. Following widespread flooding triggered by the September 2023 controlled spillage of the Akosombo and Kpong dams in Ghana, which led to massive flooding, we assessed the microbial quality of water bodies in communities downstream of the Volta River and associated public and ecosystem health implications. In July 2024, water was collected from multiple source types across four downstream townships (Asutsuare, Aveyime, Battor, and Mepe), including river sections, ponds, puddles, wells, boreholes, and canal-associated sites. Samples were filtered, microbial DNA was extracted, and bacterial community composition was profiled using 16S rRNA gene sequencing, with downstream bioinformatics analyses used to characterize microbial diversity, assess spatial variation across sites, and stratify detected taxa according to ecological origin and public health risk. Sequence data obtained from 17 out of 22 samples revealed communities dominated by Proteobacteria with frequent co-occurrence of Bacteroidetes and Firmicutes. Across all sites, 56 microbial taxa and 118 microorganisms were detected. Community composition varied by location and water source type, with bank and mid-river sites generally exhibiting higher richness than puddles and a canal drain. Thirteen samples contained site-specific taxa, indicating marked spatial heterogeneity within and between communities. Microorganisms of public health relevance were frequently detected, including enteric-associated bacteria such as Escherichia coli, Salmonella enterica, Shigella species, and Cronobacter sakazakii, as well as opportunistic and healthcare-associated bacteria including Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. Higher-risk organisms were most widely distributed in Mepe and Battor, including in groundwater-associated sources. These findings provide molecular evidence of persistent, spatially heterogeneous microbial hazards months after flooding, underscoring continued exposure risks in downstream communities and the need for targeted water safety interventions and risk-informed public health responses. - Source: PubMed
Publication date: 2026/07/02
Kpeli Grace SemabiaAgyirey-Kwakye PrinceLivingstone Counseller NutifafaCudjoe Lillian TeyeDotse Emmanuel EdemNyarko EbenezerZar EbenezerNyarko-Otu ObedAgbogli Hubert KwameGlilekpeh GodwinKorankye SolomonEssandoh PriscillaKabotso Daniel Elorm - Otosclerosis is a common cause of conductive hearing loss thought to result from dysregulated bone remodeling in the embryonic tissues of the globuli interossei. Both familial and sporadic cases have been reported. To date, 10 published loci and four genes ( (), (), , ) have been identified in autosomal dominant families. Using a combined genetic and genomics approach in five affected siblings, we identified a nonsense mutation in Karyopherin subunit α7 (, c.49C>T, p.R17X), the newest of the importin-α family of nuclear transporters. KPNA7 is a key maternal factor involved in the classical transport of NLS-containing cargo proteins, active during early embryonic cleavage events and zygotic genome activation. So far, 377 cargo proteins associated with KPNA7 have been identified. Recessive variants cause skeletal abnormalities, epilepsy, intellectual disabilities and preimplantation embryo arrest (PREMBA). A closer look at the genes reveals their involvement in endochondral ossification signaling pathways. We explore how KPNA7 haploinsufficiency in the embryonic tissues of the otic capsule may cause dysregulated bone remodeling. This study expands the phenotypic spectrum of KPNA7 and provides new insights into the pathobiology of otosclerosis. - Source: PubMed
Publication date: 2026/05/30
Benteau TammyAbdelfatah NellyGriffin AnnePenney CindyHu PingzhaoStanton Susan GZhai GuangjuMaheu MaximeFrench Curtis RYoung Terry-Lynn - Osteoporosis is a highly heritable metabolic bone disorder characterized by low bone mass and increased fracture risk. However, the causal genes underlying disease susceptibility remain incompletely understood. In this study, we employed a summary-data-based Mendelian randomization (SMR) framework to identify genes with potential causal effects on osteoporosis by integrating genome-wide association study summary statistics from the FinnGen consortium with multilayer molecular quantitative trait loci (xQTL) data, including expression, splicing, methylation, and protein QTLs across multiple tissues (106 xQTL datasets in total). The heterogeneity in dependent instruments (HEIDI) test was applied to distinguish pleiotropic associations from linkage disequilibrium-driven effects. Functional characterization was further conducted using Gene Ontology and KEGG pathway enrichment analyses, protein-protein interaction network construction, drug-gene enrichment analysis, and molecular docking. Using this integrative approach, we identified 15 high-confidence genes - CEP112, CKB, GID4, MEOX1, MEPE, PPP6R3, RGS9, RSPO3, SERPINA1, SFRP4, SOST, SPP1, SREBF1, TOM1L2, and ZBTB48 - showing evidence of causal associations with osteoporosis after stringent multiple-testing correction and HEIDI filtering. These genes included established regulators of bone metabolism as well as novel candidates involved in metabolic regulation and signal transduction. Enrichment analyses highlighted pathways related to Wnt and bone morphogenetic protein signaling, extracellular matrix organization, and metabolic processes, while network analysis revealed substantial functional connectivity among the prioritized genes. In addition, drug-gene enrichment analysis prioritized β-carotene, apocarotenal, and bezafibrate as potential therapeutic candidates, with molecular docking supporting stable interactions with key protein targets. Overall, this study provides robust genetic evidence for causal molecular regulators of osteoporosis and highlights potential therapeutic targets, offering a clinically relevant resource for future functional validation and translational research. - Source: PubMed
Yu FeiWan XiwenQiu Jiaxuan