EphB4, Mab anti_; Clone AB107
- Known as:
- EphB4, Mab anti_; Clone AB107
- Catalog number:
- AB107-1000
- Product Quantity:
- 1000 ug.
- Category:
- -
- Supplier:
- Accu
- Gene target:
- EphB4 Mab anti_; Clone AB107
Related genes to: EphB4, Mab anti_; Clone AB107
- Gene:
- EPHB4 NIH gene
- Name:
- EPH receptor B4
- Previous symbol:
- HTK
- Synonyms:
- Tyro11
- Chromosome:
- 7q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 1994-12-15
- Date modifiied:
- 2016-10-05
- Gene:
- GOLGB1 NIH gene
- Name:
- golgin B1
- Previous symbol:
- -
- Synonyms:
- GCP, GCP372, giantin, GOLIM1
- Chromosome:
- 3q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 1997-11-05
- Date modifiied:
- 2016-10-05
- Gene:
- SERPINA1 NIH gene
- Name:
- serpin family A member 1
- Previous symbol:
- PI
- Synonyms:
- AAT, A1A, PI1, alpha-1-antitrypsin, A1AT, alpha1AT
- Chromosome:
- 14q32.13
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2016-10-05
- Gene:
- SERPINB1 NIH gene
- Name:
- serpin family B member 1
- Previous symbol:
- ELANH2
- Synonyms:
- EI, PI2, anti-elastase
- Chromosome:
- 6p25.2
- Locus Type:
- gene with protein product
- Date approved:
- 1993-07-27
- Date modifiied:
- 2016-04-06
Related products to: EphB4, Mab anti_; Clone AB107
Related articles to: EphB4, Mab anti_; Clone AB107
- Ovarian cancer remains challenging to treat because of late diagnosis, heterogeneity, and poor response to existing therapies. Here, we present a streamlined and scalable extracellular vesicle (EV) engineering approach that enables efficient cargo packaging and enhances targeted cargo delivery to ovarian tumors in vivo. Systematic comparison of vesicle-anchoring domains identifies an optimized scaffold for loading bioluminescent cargo into EVs. We characterized EV production from five cell lines commonly used for biopharmaceutical manufacturing and selected and stably engineered ExpiCHO cells as a robust, large-scale source of engineered EVs (eEVs). To achieve molecular targeting, the transmembrane scaffold Δ688 PTGFRN is modified to display tissue-targeting ligand Ephrin-B2 (EB2) on the EV surface, exploiting its high-affinity interaction with the Eph receptor B4 (EphB4), which is overexpressed in advanced ovarian carcinoma. Following systemic administration, these eEVs carrying bioluminescent cargo preferentially accumulate in EphB4-positive cells in vivo and permit non-invasive, spatiotemporal tracking via cargo-mediated bioluminescence resonance energy transfer. In patient-derived xenograft models with differential EphB4 expression, Ephrin-B2-displaying eEVs show selective localization to EphB4-positive tumors and report intratumoral cargo distribution. This work establishes a translational strategy for large-scale eEV production, advancing EV-based delivery platforms for precision targeting of EphB4-expressing ovarian cancer. - Source: PubMed
Publication date: 2026/04/23
Godbole NiharLai AndrewQuinn AlexanderGillard MarianneGuanzon DominicScholz-Romero KatherinSalas-Burgos AlexisDapremont Bastián LilloLourie RohanChetty NavenLobb RichardBeecher KateReed Amy E McCartFerguson KaltinSun BiaoHe YaowuHooper John DSalomon Carlos - Chimeric antigen receptor (CAR)-T cells generated using ephrin-B2, the natural ligand for ephrin type-B receptor 4 (EphB4), have demonstrated antitumor activity; however, their efficacy remains unvalidated. Therefore, we evaluated the dual-targeting antitumor ability of these cells and confirmed their efficacy against lung adenocarcinoma. First, we evaluated EphB4 and EphA2 expression in samples from 74 patients with lung adenocarcinoma using immunohistochemistry. Next, EphB4 CAR-T cells were generated via piggyBac-mediated gene transfer, and their phenotype was evaluated. Subsequently, we conducted an antigen stimulation assay to assess the bispecificity of the EphB4 CAR-T cells. Finally, the antitumor effects of EphB4 CAR-T cells on lung adenocarcinoma cell lines were assessed. EphB4 and EphA2 positivity in lung adenocarcinoma samples was 93% and 100%, respectively. EphB4 CAR-T cells were successfully generated with high CAR positivity and a high proportion of naïve/stem cell memory-like T cells. In the antigen stimulation assay, the cells responded in an antigen-specific manner after stimulation with both EphB4 and EphA2 proteins. In the co-culture experiment, EphB4 CAR-T cells significantly suppressed the growth of all four lung adenocarcinoma cell lines compared to mock-T cells. In vivo, mice treated with EphB4 CAR-T cells displayed a significantly lower tumor burden than those treated with either CD19 CAR-T cells or phosphate-buffered saline. Additionally, mice treated with EphB4 CAR-T cells survived significantly longer than those in the other groups. In conclusion, ligand-based EphB4 CAR-T cells are bispecific, targeting both EphB4 and EphA2. Furthermore, these cells exert significant antitumor effects against lung adenocarcinoma. - Source: PubMed
Publication date: 2026/04/21
Kumeda HirotakaHirabayashi KoichiMishima ShujiMorita KotaroFurui YuFujioka MarinaNakajima TomoyukiTanaka MiyukiSato YoshinoriUehara TakeshiYagyu ShigekiShimizu KimihiroNakazawa Yozo - This study aimed to assess the detection rate and spectrum of pathogenic variants (PVs) and candidate variants (variants of uncertain significance, VUS) in AVM patients. - Source: PubMed
Publication date: 2026/04/16
Schmidt Vanessa FSchanze DennyBrill RichardLoeser Julius HUller WibkeDoppler MichaelCangir ÖzlemHengst SusanneVielsmeier VeronikaPech MaciejObereisenbuchner FlorianSchirren MirjamSint AlenaPuhr-Westerheide DanielDeniz SinanWeiß Jakob B WHäberle BeateHartel AlexandraFröba-Pohl AlexandraHaehl JuliaHolm AnnegretSporns Peter BScherf ThomasRicke JensLassmann SilkeSeidensticker MaxWohlgemuth Walter AKimm Melanie AZenker MartinWildgruber MoritzKapp Friedrich G - The receptor tyrosine kinase EphB4 is frequently overexpressed in epithelial cancers, including prostate cancer (PCa). SUMOylation is a post-translational modification that influences protein interactions, localisation and stability. This study investigated how SUMOylation regulates EphB4 localisation, stability and function in PCa. - Source: PubMed
Publication date: 2026/04/15
Maharaj Mohanan Sada NandMertens-Walker IngaLisle Jessica EHerington AdrianStephens CarsonChai MelissaMeutermans WimStephenson Sally-Anne - Capillary malformation-arteriovenous malformation (CM-AVM) is an inherited autosomal dominant vascular disorder associated with RAS p21 protein activator () or EPH receptor B4 () mutations. We aimed to investigate the clinical features of eight Chinese families with CM-AVM and the genetic characteristics of or gene variants. - Source: PubMed
Publication date: 2026/03/27
Lin YanyanDong ShuyanZhu ChanghuaDong LinxinLin LihangXiao Xuemin