EEF2 antibody Polyclonal Antibodies Primary antibodies
- Known as:
- EEF2 (anti-) Polyclonal Antibodies Primary antibodies
- Catalog number:
- orb34307
- Product Quantity:
- 5
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- EEF2 antibody Polyclonal Antibodies Primary antibodies
Related genes to: EEF2 antibody Polyclonal Antibodies Primary antibodies
- Gene:
- EEF2 NIH gene
- Name:
- eukaryotic translation elongation factor 2
- Previous symbol:
- EF2
- Synonyms:
- EEF-2
- Chromosome:
- 19p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1991-03-11
- Date modifiied:
- 2015-09-11
Related products to: EEF2 antibody Polyclonal Antibodies Primary antibodies
Related articles to: EEF2 antibody Polyclonal Antibodies Primary antibodies
- Newcastle disease (ND) caused by Newcastle disease virus (NDV) infection is a highly contagious avian disease that causes substantial threat to the global poultry industry. Emodin (EMO), a member of the free anthraquinone compounds derived from traditional Chinese medicines, is known for its antibacterial, antiviral, antitumor, and antioxidant activity. However, the anti-NDV activity and potential mechanism of action of EMO remains unknown. In this study, EMO was found to exert a remarkable anti-NDV activity in a dose- and phase-dependent manner. In addition, EMO restricted NDV replication mainly by affecting the production of viral proteins, but not by inhibiting the viral RNA synthesis and transcription. Moreover, the EMO treatment-induced reduction of viral proteins was correlated well with the increased phosphorylation level of eukaryotic elongation factor 2 (eEF2) in NDV-infected cells, which was further confirmed by inhibition of eEF2 kinase (eEF2K) activity with pharmacological inhibitors NH125 and A-484954, respectively. Meanwhile, multiple signaling cascades, including p38 MAPK, mTORC1/p70 S6K, and ERK1/2/p90 RSK1, were found to regulate the phosphorylation levels and activities of eEF2K and eEF2 by which EMO reduced viral protein production and inhibited NDV replication. Furthermore, molecular docking combined with protein-protein interaction analysis showed that EMO could form stable hydrogen bond structures with the NDV N protein at the Lys32 and Leu225 residues, and attenuate its interaction with eIF4E to decrease the production of viral proteins. These findings reveal the anti-NDV effect and the underlying mechanism of EMO, offering a potential natural compound for the development of antiviral strategies against NDV. - Source: PubMed
Publication date: 2026/05/25
Wu WenjieXia RongjingMa SiHu ZengleiDuan Zhiqiang - We utilized the nedicistrovirus (NediV) intergenic region (IGR) internal ribosomal entry site (IRES)-mediated, initiation factor-independent translation initiation system and determined high-resolution structures of 80S ribosome complexes with the NediV IRES in various functional states, including binary complexes, aminoacyl-transfer RNA (tRNA)-bound complexes, and complexes with elongation factor eEF2. In binary complexes, the NediV IRES primarily occupies the ribosomal P site, exhibiting conformational flexibility and engaging the ribosome at multiple interaction sites. Upon translocation, the IRES undergoes structural rearrangements, including destabilization of its PKI domain, facilitating the transition to canonical elongation. Crucially, we captured an eEF2-bound complex, along with an eEF1A-bound failed decoding complex featuring a mismatched tRNA, the latter representing the first instance of a canonical elongation complex visualized in the presence of a natural, hydrolysable nucleotide and without the addition of any trapping agents. These findings provide a comprehensive structural overview of IGR IRES-mediated translation initiation and its transition to elongation, revealing key mechanistic details of viral translation and proofreading. - Source: PubMed
De SwastikAltomare Clara GAbaeva Irina SDadhwal PrikshatGarg PriyankaAcosta-Reyes FranciscoBrown Zuben PPestova Tatyana VHellen Christopher U TFrank Joachim - Digital PCR (dPCR) enables absolute, precise nucleic acid quantification without the need for standard curves. Still, technical variability across the sample life cycle remains a challenge in complex multi-organ preclinical studies. This study evaluated the necessity of internal reference genes (i.e., housekeeping genes; HKGs) for normalizing reverse transcription (RT)-dPCR data from recombinant adeno-associated virus (rAAV) studies in mice and non-human primates (NHPs). HKGs were ranked using statistical analysis and further evaluated with the BestKeeper and geNorm algorithms. In mice, , , and were identified as the most stable HKGs. Normalization to a single HKG () consistently and markedly reduced transgene-expression variability across tissues. In NHPs, and demonstrated the highest stability. Importantly, multiplexing HKGs allowed to differentiate technical from biological variation, thereby mitigating common assay pitfalls. Collectively, these findings underscore that normalization is essential for reliable inter- and intra-group comparability in RT-dPCR. We strongly recommend multiplexing at least two medium-expression, validated HKGs to derive a robust normalization factor, thereby significantly improving accuracy in complex preclinical settings. - Source: PubMed
Publication date: 2026/04/16
Mackeben KlausMüller SarahDolim KimberlyOtteneder Michael BRos FrancescaFakhiri Julia - In June of 2022, 3 creole wrasses, Bodianus parrae, from a public display aquarium were submitted for diagnostic examination at Mississippi State University's College of Veterinary Medicine. Fresh myxospores recovered from the intestines were morphologically consistent with previous accounts of Enteromyxum leei (Diamant, Lom & Dyková, 1994). Molecularly, myxospores from the present study were 99.7-100% and 99.7-99.8% similar to publicly available E. leei sequences at 18S small subunit ribosomal (18S) and 28S large subunit ribosomal (28S) genes, respectively. Internal transcribed spacer region 1 (ITS1), 5.8S ribosomal rRNA gene (5.8S), and internal transcribed spacer region 2 (ITS2) sequences were 95.8-98.0%, 100%, and 99.9% similar, respectively, to the only publicly available data from these regions from E. leei. Eukaryotic elongation factor 2 (EEF2) sequences were 98.8% similar to the available EEF2 sequence of E. leei in GenBank. Lastly, cytochrome c oxidase subunit 1 (COI) sequences were 98.6% similar to previously published data. Bayesian phylogenetic analyses using both single locus 18S sequence data, concatenated 18S, 28S, and EEF2 data, and concatenated 18S, 28S, and COI amino acid sequences from each of the 3 wrasses grouped E. leei from the present study with previously published E. leei sequences with high Bayesian and maximum likelihood support. This is the first account of E. leei from B. parrae. Histological assessment reveals pathological changes to intestinal epithelia consistent with previous accounts of E. leei, with 18S, ITS1, 5.8S, ITS2, 28S, EEF2, and COI sequence data providing greater phylogenetic resolution of this enigmatic species. These morphological and molecular data from an enteromyxosis outbreak in a mixed-species exhibit at a public aquarium offer robust species-level identification of the causative agent, E. leei. - Source: PubMed
Publication date: 2026/05/13
Woodyard Ethan TStilwell Justin MPerry Sean MDelaune Alexa JStilwell Natalie KRosser T GrahamNguyen Jonah AGriffin Matt J - The river buffalo is an economically important livestock species supplying milk and meat. However, a multi-tissue transcriptomic atlas for the key dairy river buffalo breeds, Murrah and Nili-Ravi, has not yet been established, and the lack of stable reference genes has hindered in-depth studies of their biological functions and the molecular mechanisms underlying key economic traits such as lactation. We established a multi-tissue gene expression atlas across 20 tissues and identified 717 housekeeping genes (HKGs), and and were further shown to be stable candidate reference genes under the conditions tested. We found 8368 tissue-specific genes (TSGs), predominantly enriched in the reproductive system. Exploratory analysis of mammary tissue (dry-period vs. public lactating samples, confounded by batch effects) revealed mammary-enriched hub genes including ; these findings are preliminary and require validation. Dynamic analysis across lactation stages (early, peak, mid-, late) identified candidate genes including and . Phenotypic data showed strong negative correlations between milk yield and protein/fat content, and a positive correlation with lactose content. However, causal or regulatory roles were not inferred due to lack of paired individual-level data. Cross-dataset comparisons are descriptive only, and are not key conclusions. In summary, this study lays the foundation for advancing research in lactation trait genetics and functional genomics in river buffalo, with novel reference genes and lactation stage-specific transcriptional dynamics as its main contributions. - Source: PubMed
Publication date: 2026/04/30
Song XinhuiWang DongLuo XierQin ChaobinLi LingYang YanyanPi YifeiDeng YanfeiCui KuiqingLi ZhipengXu WeiLiu Qingyou