EEF2 antibody Polyclonal Antibodies Primary antibodies
- Known as:
- EEF2 (anti-) Polyclonal Antibodies Primary antibodies
- Catalog number:
- orb34307
- Product Quantity:
- 5
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- EEF2 antibody Polyclonal Antibodies Primary antibodies
Related genes to: EEF2 antibody Polyclonal Antibodies Primary antibodies
- Gene:
- EEF2 NIH gene
- Name:
- eukaryotic translation elongation factor 2
- Previous symbol:
- EF2
- Synonyms:
- EEF-2
- Chromosome:
- 19p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1991-03-11
- Date modifiied:
- 2015-09-11
Related products to: EEF2 antibody Polyclonal Antibodies Primary antibodies
Related articles to: EEF2 antibody Polyclonal Antibodies Primary antibodies
- Eggs serve as an indispensable global nutritional resource, sustaining the economic foundation of the commercial poultry industry. To meet this continuous demand, egg formation involves an exceptionally energy-intensive biological process requiring continuous yolk precursor synthesis, which imposes a massive metabolic burden on laying hens. However, the comprehensive metabolic differences across the gut-liver-adipose axis between peak laying hens with high or low egg production remain incompletely characterized. A total of 180 healthy Hy-Line Brown laying hens (45-week-old) were continuously fed and monitored for production performance over a 6-week period. Following the exclusion of individuals with extremely low egg production (≤10 eggs during the 6-week monitoring period; n = 5) and candidate hens that repeatedly produced unqualified eggs over multiple weeks (n = 20), the remaining hens were ranked by 6-week average laying rate and allocated into FH (high-production hens at 50 weeks of age, n = 15) and FL (low-production hens at 50 weeks of age, n = 14) groups. Initial body weight did not differ significantly between groups (FH: 1936 ± 29.0 g; FL: 1894 ± 48.2 g; P-value = 0.446). We integrated transcriptomic, untargeted metabolomic, targeted bile acid metabolomics, and microbiome (16S rRNA and metagenomic) profiles to characterize comprehensive metabolic changes across the gut-liver-adipose axis associated with divergent egg-production phenotypes. The results showed that: (1) FH hens exhibited higher serum APOB and lower conjugated bile acids (TCDCA, TCA, and THDCA), with hepatic upregulation of FASN, PPARA, CPT1A, and VTG1 along with downregulation of CYP7A1, CYP7B1, CYP8B1, and CYP27A1; (2) intersecting module hub genes (MHGs) with differentially expressed genes (DEGs) identified 354 upregulated and 299 downregulated core genes, with EEF2 identified as the primary hepatic downregulated hub gene; (3) in abdominal fat, GSEA revealed significant enrichment in fatty acid transport (NES = 1.54), long-chain fatty acid metabolic process (NES = 1.39), and steroid hormone biosynthesis (NES = 1.85), accompanied by significant downregulation of ANGPTL4 and upregulation of HSD3B1, VTG1, VTG2, and VTG3; (4) ileal mucosal transcriptomics identified 619 DEGs (502 upregulated), with GSEA highlighting enrichment in cell junction organization (NES = 1.52) and tube morphogenesis (NES = 1.39), which were further categorized into functional modules including enteric synaptic signaling, epithelial adhesion, mucosal vascularization, and tissue renewal; (5) the functional profile of the ileal microbiota in FH hens showed enrichment of functions related to complex carbohydrate degradation and carbohydrate-binding modules, with keystone taxa including Blautia and Bifidobacterium associated with production and lipid markers. Collectively, these findings suggest that high egg production during the peak laying period is associated with coordinated metabolic differences across the liver, abdominal fat tissue, and intestine. The observed profiles included reduced hepatic translation-related and primary bile acid synthesis-related signatures, adipose endocrine-related changes and microbial functional potential related to carbohydrate utilization and antioxidant-related functional potential. These findings provide candidate multi-omics features for precision nutritional strategies and genetic improvement in commercial poultry. - Source: PubMed
Publication date: 2026/07/27
Ma JunjieQin KailongQiao ZhihaoRen ZhouzhengYang XiaojunLiu Yanli - Protein synthesis is a highly energy-dependent process that consists of initiation, elongation, termination, and ribosome recycling. Historically, initiation has been viewed as the major site of translation regulation. However, growing recognition of elongation as a regulatory node has highlighted the importance of eukaryotic elongation factor (eEF)1A-dependent decoding, eEF2-catalyzed ribosome translocation, conditional elongation factors, ribosome speed, and tRNA dynamics. In neurons, these mechanisms are especially important because protein synthesis must be regulated across highly polarized cellular compartments. In this review, we summarize evidence from diverse model systems showing that tightly regulated translation elongation supports cellular function and viability, whereas disrupted elongation contributes to dysfunction and disease throughout the nervous system. - Source: PubMed
Publication date: 2026/08/11
Mikesell Alexander RWillis Anne ELoerch SarahCampbell Zachary T - As a non-coding RNA (lncRNA), the RNA component of mitochondrial RNA processing endoribonuclease (RMRP) is implicated in ribosome biogenesis. In recent years, its role in the neurodegenerative system has been reported; however, the molecular mechanism underlying RMRP-mediated neuroprotective effects remains elusive. In the present study, we identified that RMRP expression is regulated by the RNA-binding protein RBM3. The overexpression of RBM3 significantly upregulated RMRP transcription in SH-SY5Y neural cells, whereas RBM3 knockdown led to a marked reduction in RMRP expression. Furthermore, RNA Immunoprecipitation (RIP) assays confirmed the potential interaction between RMRP and RBM3. We then investigated the functional significance of RMRP regulated by RBM3 in Parkinson's disease (PD) cell models. Exogenous overexpression of RMRP strongly attenuated cytotoxicity induced by neurotoxins rotenone (ROT) and MPP in SH-SY5Y cells, as evidenced by decreased levels of cleaved poly ADP-ribose polymerase 1 (PARP1) and enhanced cell viability. Given that RBM3 exerts robust neuroprotective effects by accelerating global protein synthesis (GPS), we hypothesized that RMRP is a key mediator of RBM3-conferred neuroprotection. Consistent with this hypothesis, RMRP overexpression enhanced the activity of eukaryotic elongation factor 2 (eEF2), a hallmark of cellular GPS. Its stimulatory effect on GPS was further validated using a puromycin incorporation assay. Collectively, our data reveal that RMRP acts as a novel effector of RBM3 in stimulating cellular GPS and conferring neuroprotective effects in SH-SY5Y cells, providing a new therapeutic target for PD. - Source: PubMed
Publication date: 2026/07/28
Ma ShuangpingLiu XiantingXie YimingHan YuxuanWang NingLi ShenxueCheng BinfengJu FeiWang Lei - Alzheimer's disease (AD), the most common form of dementia, remains without effective therapies. Dysregulated eukaryotic elongation factor 2 kinase/eukaryotic elongation factor 2 (eEF2K/eEF2) pathway leads to aberrant protein synthesis and impaired neuronal function in AD, positioning this axis as a promising therapeutic target. However, effective pharmacological modulators of eEF2K/eEF2 remain limited. Betulinaldehyde (Betu), an active ingredient derived from traditional Chinese medicine, remains to be thoroughly evaluated as a potential neuroprotective agent. - Source: PubMed
Wang ChaoqunHan XiaoheLin YaliPan YanMiao QiupingWang YapingWang Shu-QinZhang ZhuWang LuyaoTang Zhi-RiPeng YinghuiYung Ken Kin-LamMa NanLu DanZhang ShiqingShi Lei - Major depressive disorder (MDD) is recognized as a pressing global public health burden. However, its molecular mechanisms remain incompletely understood. - Source: PubMed
Publication date: 2026/06/19
Fang HaiyanZhu GuangjunChen JinZou Tao