RAB-14 antibody Polyclonal Antibodies Primary antibodies
- Known as:
- RAB-14 (anti-) Polyclonal Antibodies Primary antibodies
- Catalog number:
- orb100703
- Product Quantity:
- 100
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- RAB-14 antibody Polyclonal Antibodies Primary antibodies
Related genes to: RAB-14 antibody Polyclonal Antibodies Primary antibodies
- Gene:
- RAB14 NIH gene
- Name:
- RAB14, member RAS oncogene family
- Previous symbol:
- -
- Synonyms:
- FBP, RAB-14
- Chromosome:
- 9q33.2
- Locus Type:
- gene with protein product
- Date approved:
- 2001-09-14
- Date modifiied:
- 2015-02-02
Related products to: RAB-14 antibody Polyclonal Antibodies Primary antibodies
Related articles to: RAB-14 antibody Polyclonal Antibodies Primary antibodies
- Tumor CMTM6 is known to regulate programmed death-ligand 1 expression and affect the cytotoxicity of infiltrating T cells, while its impact on natural killer (NK) cells and tumor malignancy remains to be fully understood. This study aimed to investigate the influences of tumor CMTM6 on NK cell infiltration and tumor progression. - Source: PubMed
Publication date: 2026/03/16
Ye FangzhouLi JianWang JiayiFan JiajunSheng XiaBei SonghuaZhang XiaohongJiang JunLi HuanqingFeng Li - Host restriction factors mediate intrinsic immunity against infections, thus serving as promising targets for host-directed therapy (HDT) against drug-resistant pathogens. While restriction factors counteracting viruses have been extensively studied, those targeting bacteria, particularly those with broad-spectrum activity, remain largely unexplored. Here, through screening for host factors promoting lysosomal acidification, a crucial process clearing pathogens, we identify the host small GTPase Rab14 as a restriction factor with broad-spectrum activity against multiple bacteria and viruses. Mechanistically, upon pathogen infections, GTP-bound Rab14 increases and binds to the calcium/calmodulin-dependent protein kinase type 2 delta (CAMK2D), suppressing CAMK2D-mediated phosphorylation of V0a1, the critical subunit determining V-ATPase localization, thus promoting V0a1 binding to the COPⅡ complex to facilitate V-ATPase trafficking from the endoplasmic reticulum to lysosomes, resulting in lysosomal acidification and pathogen clearance. Taken together, our data demonstrate an unrecognized intrinsic immune mechanism mediated by Rab14-CAMK2D-V-ATPase axis, which might be a promising target for infectious diseases. - Source: PubMed
Publication date: 2026/03/02
Lei ZehuiQiang LihuaGe PupuQiang YuyunSun TergelChai QiyaoWang YiruLv ShanQiu ChanggenLu ZheZhao MengyuanZhao ZhuoWu YouZhang XinwenZhong YanzhaoLi BingxiZhang LingqiangWang JingLiu Cui Hua - As health awareness grows, more people are turning to weight loss-yet excessive slimming or dietary imbalance is quietly triggering a wave of fertility problems. Researches show that low BMI or excessive exercise can negatively impact fertility, but its specific cellular mechanism remain unclear. In this study, we investigated how adipose tissue influences ovarian function under energy-restricted conditions by establishing a peri-ovarian adipose tissue (POAT) removal mouse model. We showed that POAT removal resulted in a significant decrease in litter size, impaired follicle development, and an increased apoptosis rate in granulosa cells (GCs). Additionally, the lipid droplets (LDs) content in the ovaries and GCs was significantly reduced. We also observed that Rab14 expression was upregulated in GCs after POAT removal. Functional experiments further showed that Rab14 overexpression significantly enhanced lipophagy activity, reduced the number and volume of LDs, and inhibited the expression of lipid droplet protein PLIN2. Meanwhile, Rab14 overexpression inhibited the phosphorylation level of AKT and induced the apoptosis of granulosa cells, suggesting that Rab14 may activate the cell death pathway mediated by lipophagy. Taken together, POAT may support ovarian function by maintaining lipid homeostasis within GCs. The role of Rab14 in the regulation of lipophagy may link local adipose-derived signaling with GCs survival and female fertility. - Source: PubMed
Zhong DanLi LiangtingZhang JuanGuo YapingSu YanZhao ShuangshuangAn HuiqingHou XiaojingJi ChenboShen Rong - Extracellular vesicles (EVs) play a pivotal role in tumor progression, influencing the tumor microenvironment. Despite significant research, the targeted analysis of EVs directly derived from primary tumors remains limited, particularly in ovarian cancer. The majority of existing studies have focused on EVs derived from peritoneal fluid (ascites), which encompasses contributions from different cell types. This study aims to isolate and characterize EVs secreted specifically by ovarian cancer spheroids derived from primary patient ascites. A three-dimensional cell culture model was employed to cultivate tumor spheroids in a defined medium, with EVs purified via differential ultracentrifugation and size-exclusion chromatography. Purified EVs were characterized by nanoparticle tracking analysis, nanoflow cytometry, and electron microscopy prior to performing high-resolution mass spectrometry. This approach allowed the identification of known cancer-associated proteins, including danger molecules, which are linked to poor prognosis. Moreover, enzyme-linked immunosorbent assay (ELISA) analysis demonstrated that the ascites abundance levels of novel candidates [RAB14 (Ras-related protein Rab-14), SCAMP3 (secretory carrier membrane protein 3), and FAM3C (FAM3 metabolism regulating signaling molecule C)] correlated with patients' progression-free survival, further validating their clinical relevance. Finally, we used the Gene Expression Profiling Interactive Analysis 2 (GEPIA2) database to compare our dataset with The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data. Thereby, we revealed a signature of three TOP genes encoding proteins within our dataset (CORO1B, LAMP2, MSLN), which were differentially expressed in ovarian cancer patients compared to healthy individuals. This study provides the first proteomic profile of EVs derived directly from primary tumor spheroids, and paves the way for a better mechanistic understanding of EV-associated proteins and for the development of biomarkers or therapeutic strategies. - Source: PubMed
Publication date: 2025/11/05
Preußer ChristianGläser MaxGraumann JohannesSzymański WitoldBachurski DanielGómez-Serrano MaríaJacob RalfReinartz Silkevon Strandmann Elke Pogge - Chronic obstructive pulmonary disease (COPD) is a common chronic respiratory disorder. Its prevention and management have become increasingly challenging owing to subtle and easily overlooked early symptoms, warranting the exploration of detection markers. Hence, this study aimed to explore COPD-related competitive endogenous RNA (ceRNA) mechanisms with notable predictive value by leveraging bioinformatics methods. Herein, Gene Expression Omnibus, lncRNASNP2, and miRDB were used to construct a COPD-related ceRNA network, which was then assessed through Kyoto Encyclopedia of Gene and Genomes pathway analysis. The targeting relationship of identified axis was confirmed through RNA pull-down and dual-luciferase reporter assays. In total, 106 patients with stable COPD and 98 patients with acute exacerbation COPD (AECOPD) were enrolled. Binary logistic regression and receiver operating characteristic curve analyses were performed to evaluate the clinical significance of the identified axis. Additionally, its role in COPD-related inflammation was investigated in BEAS-2B cells treated with cigarette smoke extract (CSE). A potential ceRNA mechanism was identified involving LINC01569, miR-4722-5p, and RAB14, where LINC01569 overexpression promoted RAB14 expression by competitively binding to miR-4722-5p. Both LINC01569 and RAB14 were highly expressed in AECOPD, making them risk factors of COPD (odds ratio [OR] = 5.100 and 8.076, respectively), with their area under the curve (AUC) values for predicting disease progression being 0.878 and 0.822, respectively. In contrast, miR-4722-5p acted as a potential protective factor (OR = 0.448), with an AUC of 0.724 for predicting AECOPD occurrence. Their serum expression strongly correlated with inflammatory markers. In CSE-treated BEAS-2B cells, silencing LINC01569 upregulated miR-4722-5p, thereby suppressing RAB14 expression and reducing pro-inflammatory factor production. Altogether, the LINC01569/miR-4722-5p/RAB14 regulatory axis represents a potential ceRNA mechanism influencing COPD progression. It demonstrates significant predictive value for disease development and plays a crucial role in COPD-associated inflammatory processes. - Source: PubMed
Xu GuangfeiWang NaWang Jianying