REC8 antibody Polyclonal Antibodies Primary antibodies
- Known as:
- REC8 (anti-) Polyclonal Antibodies Primary antibodies
- Catalog number:
- orb100627
- Product Quantity:
- 100
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- REC8 antibody Polyclonal Antibodies Primary antibodies
Related genes to: REC8 antibody Polyclonal Antibodies Primary antibodies
- Gene:
- REC8 NIH gene
- Name:
- REC8 meiotic recombination protein
- Previous symbol:
- REC8L1
- Synonyms:
- Rec8p, kleisin-alpha
- Chromosome:
- 14q12
- Locus Type:
- gene with protein product
- Date approved:
- 2003-09-29
- Date modifiied:
- 2016-01-15
Related products to: REC8 antibody Polyclonal Antibodies Primary antibodies
Related articles to: REC8 antibody Polyclonal Antibodies Primary antibodies
- Meiosis is a conserved yet evolutionarily varied process underpinning sexual reproduction in eukaryotes. In the malaria parasite Plasmodium, meiosis is unconventional: it occurs immediately after fertilisation (post-zygotic) and must be coordinated with the transformation of the zygote into a motile ookinete. The mechanisms synchronising these meiotic and morphogenetic programmes remain unknown. Here, we identify the Plasmodium berghei NIMA-related kinase NEK4 as a key regulator that couples meiotic initiation with zygote morphogenesis. Using ultrastructure expansion microscopy, we show that NEK4 accumulates at the microtubule-organising centre (MTOC) and the apical polar complex (APC) shortly after fertilisation, preceding the assembly of perinuclear and cortical microtubules. We reveal that Plasmodium zygotes undergo MTOC-associated nuclear migration, analogous to the meiotic nuclear movement in fission yeast. Deletion of the Pbnek4 gene results in complete developmental arrest: MTOC duplication and microtubule formation are blocked, chromatin remains uncondensed, and nuclear migration and cell polarity fail to establish. Transcriptomic and phosphoproteomic analyses reveal that absence of NEK4 causes a collapse in transcriptional and phosphoregulatory networks governing meiosis and cytoskeletal organisation, leading to reduced expression and phosphorylation of important players, including HOP1, REC8, and AP2-O. These findings establish NEK4 as a key regulator driving meiotic entry and zygote maturation. - Source: PubMed
Publication date: 2026/05/12
Yanase RyujiHair MollyZeeshan MohammadFerguson David J PBrady DeclanPasquarello CarlaBottrill AndrewBhanvadia SuhaniNeal ArmundTromer Eelco CLe Roch Karine GHainard AlexandreHolder Anthony AVaughan SueGuttery David STewari Rita - Non-homologous end joining (NHEJ) is an error-prone but efficient primary repair pathway for DNA double-strand breaks (DSBs) during mitosis. A critical unresolved question is whether NHEJ acts as a backup when homologous recombination (HR) is impaired during meiosis. Here, we integrated biochemical, cytological, and genetic approaches to dissect the biological role of KU70/KU80, the core component of the NHEJ machinery, in both mitotic and meiotic DSB repair in rice (Oryza sativa). Biochemical analysis confirmed that KU70 and KU80 form a stable heterodimer. KU deficiency caused hypersensitivity to bleomycin in somatic cells, underscoring its essential function in mitotic DSB repair. KU70 localized to meiotic telomeres but was dispensable for normal meiotic progression and DSB repair efficiency. Moreover, KU deficiency neither altered the aberrant chromosome associations in HR-defective mutants (com1, rec8, meica1, hus1, and rad1) nor genetically interacted with key HR factors (ZIP4 and MER3). Taken together, our findings establish rice meiosis operates under an HR-dominant DSB repair regime independent of KU-mediated NHEJ. This stands in striking contrast to the critical role of NHEJ in mitotic DSB repair and clearly demonstrates that KU-mediated NHEJ does not serve as a backup repair pathway for impaired HR during plant meiosis. - Source: PubMed
Publication date: 2026/05/03
Zhao YangziWang BingxinPang YunfeiYou HanliCao LeiZhong WeijieWang YuhaoZhang JiaruiChen YangGong ZhiyunLi YafeiCheng Zhukuan - Cohesin is a ring-shaped protein complex that mediates sister-chromatid cohesion (SCC) to ensure accurate chromosome segregation during mitosis and meiosis. In Saccharomyces cerevisiae, cohesin consists of four core subunits-Smc1, Smc3, Scc1/Mcd1, and Scc3. During meiosis, the mitotic α-kleisin Scc1/Mcd1 is replaced by the meiosis-specific α-kleisin Rec8. Rec8-containing cohesin is essential for multiple meiotic processes, including chromosome morphogenesis, homologous recombination, axis and synaptonemal complex formation, SCC, and transcriptional regulation. While stable association of Rec8-cohesin with chromatin is required to maintain SCC from premeiotic S phase through anaphase II, dynamic chromatin association is thought to underlie its roles in recombination, chromosome architecture, and transcription via loop extrusion. Whether distinct stable and dynamic pools of Rec8-cohesin coexist during meiosis and how their functions are partitioned remained unclear. Here, we employed an anchor-away strategy to conditionally deplete only the dynamic pool of Rec8-cohesin from the nucleus while preserving the stable pool. Selective depletion reduced sporulation efficiency and spore viability without compromising SCC. Calibrated ChIP-seq revealed a genome-wide reduction in Rec8-cohesin levels rather than locus-specific loss. Functional analyses demonstrated that the dynamic pool of Rec8-cohesin is required for efficient meiotic recombination, establishment of meiosis-specific chromosome architecture and synaptonemal complex formation, and proper transcriptional regulation of key meiotic regulators. In contrast, the stable pool alone was sufficient to maintain spindle pole body cohesion. Together, our findings demonstrate the existence of two functionally distinct pools of Rec8-cohesin during yeast meiosis. - Source: PubMed
Publication date: 2026/04/24
Paliwal SheetalDey ParthaKumari AkritiSadasivam KirithikaJoshi SameerRaghuvanshi RitikaGoyal RohitSanyal KaustuvNishant K TShinohara AkiraMehta Gunjan - Oocyte meiosis determines chromosomes that are transmitted from the mother to the next generation. The molecular mechanisms underlying meiotic chromosome segregation are organized in distinct chromosomal regions-the core centromere, peri-centromere, and chromosome arms. A common molecular basis for mechanisms operating in these distinct regions is Rec8 cohesin, together with cooperating regulatory pathways. This Review describes Rec8-cohesin-based mechanisms operating in each chromosomal region and discusses how these mechanisms work coordinately to achieve chromosome segregation during meiosis I, focusing primarily on insights from studies using mouse oocytes as a model system. Based on this molecular understanding, it further discusses how age-associated decline of chromosomal Rec8 cohesin can lead to chromosome segregation errors, a major cause of infertility, miscarriage, and congenital disorders. - Source: PubMed
Publication date: 2026/04/25
Kitajima Tomoya S - The first division of meiosis is unique in its capacity to halve the ploidy of future gametes. To this end, one key innovation compared with mitosis is the monopolar orientation of the pairs of sister kinetochores required for the proper separation of homologs at meiosis I. How monopolar orientation is imposed is unclear and seems to vary in eukaryotes. Here, we performed a forward genetic screen in Arabidopsis thaliana, specifically designed to identify the molecular components imposing monopolar orientation, based on mutants' ability to restore fertility in spo11 osd1 haploid plants. We show that monopolar orientation involves all four cohesin subunits (REC8, SCC3, SMC1, and SMC3), the cohesion establishment factors CTF18 and DCC1, the cohesin protectors SGO1/2 and PATRONUS (PANS1), the inner kinetochore protein CENP-C, and the deSUMOylase SUMO PROTEASE RELATED TO FERTILITY 2 (SPF2). The mutants show bipolar orientation of achiasmatic chromosomes; however, monopolar orientation is maintained in the presence of crossovers, despite most of them displaying the splitting of sister kinetochores and reduced levels of cohesin at metaphase I. Taken together, the findings demonstrate that cohesion establishment and maintenance, kinetochore function, and deSUMOylation, together with crossovers, promote monopolar orientation in plants and support a cohesion-driven model of kinetochore orientation at meiosis I that is conserved across kingdoms. - Source: PubMed
Publication date: 2026/03/31
Singh Dipesh-KumarMahlandt AlexanderDurand StephanieJolivet SylvieWalkemeier BirgitTaochy ChristelleSolier VictorDerkacheva MariaSiddiqi ImranCromer LaurenceMercier Raphael