UTS2D antibody Polyclonal Antibodies Primary antibodies
- Known as:
- UTS2D (anti-) Polyclonal Antibodies Primary antibodies
- Catalog number:
- orb100386
- Product Quantity:
- 100
- Category:
- -
- Supplier:
- Biorb
- Gene target:
- UTS2D antibody Polyclonal Antibodies Primary antibodies
Related genes to: UTS2D antibody Polyclonal Antibodies Primary antibodies
- Gene:
- UTS2B NIH gene
- Name:
- urotensin 2B
- Previous symbol:
- UTS2D
- Synonyms:
- URP, U2B
- Chromosome:
- 3q28
- Locus Type:
- gene with protein product
- Date approved:
- 2005-02-25
- Date modifiied:
- 2014-11-19
Related products to: UTS2D antibody Polyclonal Antibodies Primary antibodies
Related articles to: UTS2D antibody Polyclonal Antibodies Primary antibodies
- The caudal neurosecretory system (CNSS) is a neuroendocrine complex unique to fish, first described in 1955. Since then, it has been hypothesized to contribute to several physiological processes, but its real functions remain largely unclear. However, so far, the many studies devoted to it agree that it could play an important role in osmoregulation. More recently, it has also been suggested that it could be involved in thermal adaptation. The zebrafish (Danio rerio) is a well-established model organism for functional studies. Yet so far, the functions of the CNSS have not been thoroughly investigated in this species. As a first step in such investigations, the present study aimed to identify environmental factors whose variations induce changes in CNSS endocrine activity. For this purpose, juvenile zebrafish were submitted to acute (2, 8, and 24 h) pH, salinity, and temperature challenges. As indicators of the CNSS endocrine activity, the expression levels of peptide hormone-encoding genes known or suspected to be synthesized in the CNSS were measured using quantitative PCR. The genes selected for this study were as follows: corticotropin-releasing hormone b (crhb), oxytocin (oxt), proenkephalin (penka and penkb), parathyroid hormone-like hormone (pthlha and pthlhb), stanniocalcin 2 (stc2a and stc2b), urotensin 1 (uts1) and urotensin 2 (uts2a and uts2b). Our findings revealed that the pH challenge affected the expression of three genes - crhb, penka, and stc2b - and the salinity challenge altered four genes - oxt, uts1, uts2a, and uts2b - while the temperature challenge modified the expression of all genes of our panel. These results indicated that the zebrafish CNSS is sensitive to changes in these environmental parameters and support the use of the zebrafish as a relevant model for studying the functions of the CNSS. - Source: PubMed
Publication date: 2025/06/11
Bichon BéréniceAlfama GladysGaillard Anne-LaureTostivint HervéPézeron Guillaume - In this work, we have analyzed the transcriptomic changes in the brainstem of male Wistar rats 2 h after an acute stress exposure. We performed duplex-specific nuclease normalization of cDNA libraries and compared the results back-to-back for the first time. Based on our RNAseq data, we selected reference genes for RT-qPCR that are best suited for acute stress experiments. Most genes were upregulated. We detected a massive shift in neuropeptide Crh, Trh,Cga, Tshb, Uts2b, Tac4, Lep and neuropeptide receptor Hcrtr1, Sstr5, Bdkrb2, Crhr2 signaling, as well as glutamate Grin3b, Grm2 and GABA Gpr156, acetylcholine Chrm4,Chrne, adrenergic Adra2b receptors expression. A strong increase in the expression of intermediate filaments Krt83/Krt86/Krt80/Krt84/Krt87/Krt4/Krt76 and motor proteins Myo7a, Klc3 was detected. Remarkably, in the absence of astrocyte activation, we also observed signs of microglial activation at this time point. Both expression of anti-inflammatory cytokines Il13, Ccl24 and pro-inflammatory cytokine receptors Il9r, Il12rb1, Tnfrsf14, Tnfrsf13c, Tnfrsf25, Tnfrsf1b were increased. In the Wnt signaling pathway, we observed increased expression of ligands-receptors Wnt1, Wnt11, Ror2 and also negative regulators Notum, Sfrp5, Sost. RNAseq results after DSN treatment correlated at a high level with RNAseq results without DSN, but there was a proportion of genes that shifted their logFC values. They are mostly rare transcripts TPM 1-10 with higher 0.5-0.9 GC content. - Source: PubMed
Publication date: 2024/09/19
Lanshakov Dmitriy ASukhareva Ekaterina VBulygina Veta VKhozyainova Anna AGerashchenko Tatiana SDenisov Evgeny VKalinina Tatyana S - Gene expression of the chick retina was examined during the early development of lens-induced myopia (LIM) using whole transcriptome sequencing. Monocular treatment of the right eyes with -10 diopter (D) lenses was performed on newly born chicks for one day (LIM-24) or two days (LIM-48), while the contralateral eyes without lenses served as controls. Myopia development was confirmed by demonstrating significant elongation of the optical axis in lens-treated eyes compared to untreated control eyes. RNA was extracted and RNA-seq was performed using the Illumina HiSeq 2000 platform. Data analysis was carried out on a Partek® Flow platform. Using screening criteria of ≥1.30-fold change and a false discovery rate <1%, 11 (five down-regulated and six up-regulated) and 35 differentially expressed genes (six down-regulated and twenty-nine up-regulated) were identified at 24 hours and 48 hours, respectively. Using another cohort for validation, Quantitative PCR confirmed significant changes in the expression of and mRNA ( <0.05) after only 24 hours of LIM treatment and numerical changes in the expression for and , which were consistent with transcriptome sequencing but did not reach statistical significance. These data suggest that concerted changes of retinal gene expression may be instrumental in the initiation of axial elongation and myopia development. - Source: PubMed
Publication date: 2022/06/13
Shan Sze WanWang Pan FengCheung Jimmy Ka WaiYu FengjuanZheng HuiLuo ShumengYip Shea PingTo Chi HoLam Thomas Chuen - Enteroendocrine cells (EECs) are the primary sensory cells that sense the gut luminal environment and secret hormones to regulate organ function. Recent studies revealed that vagal afferent neurons are connected to EECs and relay sensory information from EECs to the brain stem. To date, however, the identity of vagal afferent neurons connected to a given EEC subtype and the mode of their gene responses to its intestinal hormone have remained unknown. Hypothesizing that EEC-associated vagal afferent neurons change their gene expression in response to the microbiota-related extracellular stimuli, we conducted comparative gene expression analyses of the nodose-petrosal ganglion complex (NPG) using specific pathogen-free (SPF) and germ-free (GF) mice. We report here that the Uts2b gene, which encodes a functionally unknown neuropeptide, urotensin 2B (UTS2B), is expressed in a microbiota-dependent manner in NPG neurons. In cultured NPG neurons, expression of Uts2b was induced by AR420626, the selective agonist for FFAR3. Moreover, distinct gastrointestinal hormones exerted differential effects on Uts2b expression in NPG neurons, where cholecystokinin (CCK) significantly increased its expression. The majority of Uts2b-expressing NPG neurons expressed CCK-A, the receptor for CCK, which comprised approximately 25% of all CCK-A-expressing NPG neurons. Selective fluorescent labeling of Uts2b-expressing NPG neurons revealed a direct contact of their nerve fibers to CCK-expressing EECs. This study identifies the Uts2b as a microbiota-regulated gene, demonstrates that Uts2b-expressing vagal afferent neurons transduce sensory information from CCK-expressing EECs to the brain, and suggests potential involvement of UTS2B in a modality of CCK actions. - Source: PubMed
Publication date: 2022/03/31
Yoshioka YutaTachibana YoshihisaUesaka ToshihiroHioki HiroyukiSato YuyaFukumoto TakumiEnomoto Hideki - Urotensin II (UII) and UII-related peptide (URP) are vasoactive peptide hormones causing strong vasoconstriction or vasodilation, depending on the type of blood vessel. In humans, the active forms are resulting from proteolytic cleavage of their inactive precursor protein. In blood plasma, a defined protease converting the inactive UII and URP precursors into their active forms has not been identified yet. Using mass spectrometry-based enzyme screening for detecting UII- and URP-converting enzymes, the human plasma fraction Cohn IV-4 was chromatographed, and the resulting fractions were screened for UII- or URP-generating activity. Plasma kallikrein (PK) as a UII- and URP-generating protease was identified. URP generation was also found for the serine protease factor XIa, plasmin, thrombin, and, to a smaller extent, factor XIIa. It was demonstrated that in the Cohn IV-4 fraction, PK accounts for a significant amount of UII- and URP-generating activity. - Source: PubMed
Publication date: 2021/11/04
Schuster RaphaelSteffen PascalDreyer BenjaminRohn SaschaSchlüter HartmutRiedner Maria