CENP_A mAb;human (3_19)
- Known as:
- CENP_A mAb;H. sapiens (3_19)
- Catalog number:
- ASAKAM-CC006E
- Product Quantity:
- 100 µg
- Category:
- -
- Supplier:
- Other suppliers
- Gene target:
- CENP_A mAb;human (3_19)
Related genes to: CENP_A mAb;human (3_19)
- Gene:
- ARMC10 NIH gene
- Name:
- armadillo repeat containing 10
- Previous symbol:
- -
- Synonyms:
- MGC3195, SVH
- Chromosome:
- 7q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2007-01-26
- Date modifiied:
- 2014-11-19
- Gene:
- ATXN7L1 NIH gene
- Name:
- ataxin 7 like 1
- Previous symbol:
- ATXN7L4
- Synonyms:
- KIAA1218, MGC33190
- Chromosome:
- 7q22.3
- Locus Type:
- gene with protein product
- Date approved:
- 2004-08-18
- Date modifiied:
- 2016-10-05
- Gene:
- CCDC107 NIH gene
- Name:
- coiled-coil domain containing 107
- Previous symbol:
- -
- Synonyms:
- MGC31967
- Chromosome:
- 9p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 2006-05-12
- Date modifiied:
- 2016-10-05
- Gene:
- CENPA NIH gene
- Name:
- centromere protein A
- Previous symbol:
- -
- Synonyms:
- CENP-A, CenH3
- Chromosome:
- 2p23.3
- Locus Type:
- gene with protein product
- Date approved:
- 1988-05-31
- Date modifiied:
- 2014-11-19
- Gene:
- CGN NIH gene
- Name:
- cingulin
- Previous symbol:
- -
- Synonyms:
- KIAA1319
- Chromosome:
- 1q21.3
- Locus Type:
- gene with protein product
- Date approved:
- 2003-07-24
- Date modifiied:
- 2016-10-05
Related products to: CENP_A mAb;human (3_19)
Related articles to: CENP_A mAb;human (3_19)
- Centromeres are essential chromosomal loci specified epigenetically by CENP-A chromatin, yet they undergo rapid sequence turnover, structural remodeling, and occasional repositioning. In this review, we integrate recent advances enabled by long-read genome assemblies and high-resolution chromatin mapping to synthesize current understanding of centromere organization across taxa. We examine how satellite repeats, transposable elements, molecular drive, and meiotic conflict generate extreme centromere diversity. We further explore how DNA methylation and H3K9me3 heterochromatin constrain CENP-A positioning, stabilize centromeric domains, and shape boundary dynamics during centromere drift, duplication, and de novo formation. Together, these perspectives show how centromeres accommodate evolutionary change while preserving the stringent requirements of faithful chromosome segregation. - Source: PubMed
Publication date: 2026/07/15
Anderson Emma MMellone Barbara G - Centromeres ensure faithful chromosome segregation despite being embedded within rapidly evolving repetitive DNA, a contradiction known as the centromere paradox. While centromere identity is defined by the histone variant CENP-A, how conserved function is maintained amid rapid DNA turnover remains unclear. Here, we generate highly contiguous genome assemblies from single individuals that, for the first time, resolve a chromosome through its centromere, linking the chromosome 3 arms within a continuous sequence. Comparative assemblies from wild-derived strains reveal extensive structural variation in pericentromeric satellites, including large-scale expansions, contractions, and sequence divergence. Despite this variation, the CENP-A-associated centromeric core exhibits conserved organization across strains. Integration of Hi-C interaction maps with sequence analyses shows that flanking satellite arrays form a spatially interacting domain that bridges both sides of the centromere, whereas adjacent arrays are more variable and show weaker interactions. These results support a model in which rapidly evolving centromeric DNA is constrained by conserved higher-order architecture, providing a framework for reconciling the rapid evolution of centromere sequence with its conserved function. - Source: PubMed
Publication date: 2026/07/01
Samano AlejandraChakraborty Mahul - Centromeres, the chromosomal loci responsible for segregation during cell division, play a key role in genome evolution and speciation. While centromere function is highly conserved and epigenetically defined by CENP-A, the underlying DNA sequences are among the most rapidly evolving. Although mammalian centromeres are typically associated with satellite DNA, we previously showed that equids carry numerous satellite-free centromeres. Here, we investigate centromere and karyotype evolution in Tapirus indicus, a non-equid perissodactyl with exceptional karyotypic plasticity. Through CENP-A ChIP-seq analysis on the same individual for which a near-gapless diploid genome assembly generated by the Vertebrate Genome Project was available, we identify 23 canonical satellite-based centromeres, two completely satellite-free centromeres, and one centromere with a very low content of satellite tracts. The unconventional centromeres arose through centromere repositioning, thereby redefining the evolutionary prevalence of satellite-free centromeres across mammals. Comparative genomic analysis uncovers evolutionary hotspots for satellite-free centromere formation across Perissodactyla. Finally, analysis of CENP-B binding shows that T. indicus displays uncoupling between CENP-A and CENP-B, a feature previously observed only in equids. These findings reveal that high centromere plasticity is not unique to equids and support a broader model in which centromere plasticity and CENP-B uncoupling contribute to karyotype evolution in mammals. - Source: PubMed
Publication date: 2026/07/07
Biundo MarialauraPiras Francesca MRapisarda EdoardoRyder Oliver ANergadze Solomon GGiulotto ElenaCappelletti Eleonora - We previously identified, using a synthetic peptide, namely peptide 4.33 (p4.33), a subgroup of anti-CENP-A antibodies (Abs) recognizing an epitope shared between the CENP-A region spanning amino acids 1-17 (Ap1-17) and the E2 component of the mitochondrial pyruvate dehydrogenase complex (PDC-E2), the major mitochondrial target autoantigen in primary biliary cholangitis (PBC). Here, we evaluated whether anti-p4.33 Ab positivity may be associates with a higher prevalence of antimitochondrial Ab (AMA) in systemic sclerosis (SSc) patients. - Source: PubMed
Favoino ElviraSantis Silvia DeLiakouli VasilikiBarbuti GiovannaPiccolo SabinaCorrado AdaVomero MartaNavarini LucaPrete MarcellaLeone PatriziaRacanelli VitoGrembiale Rosa DanielaRuscitti PieroCantatore Francesco PaoloCiccia FrancescoGiacomelli RobertoPerosa Federico - In most animals and fungi, centromere identity and function depend on the Scm3/Holliday junction recognition protein (HJURP) chaperone, which deposits CENPA at centromeres. However, Scm3/HJURP orthologs appeared to be missing in insects, nematodes, many vertebrates, and other metazoans, suggesting radical chaperone replacement in these lineages. Here, we combine remote homology detection, AlphaFold-based structural modeling, and functional genetics in zebrafish and to identify previously unknown Scm3/HJURP orthologs that localize to centromeres and whose loss causes catastrophic mitotic failure. We further show that CAL1, long considered a functional analog, is instead a highly diverged Scm3/HJURP ortholog. Despite rapid primary-sequence divergence, predicted and known structures reveal a broadly conserved CENPA-H4-binding scm3 fold across fungi, vertebrates, nematodes, insects, and most metazoans. Our work demonstrates how rapid divergence can obscure the broad conservation of essential centromere machinery and provides a generalizable strategy for unmasking missing orthologs. - Source: PubMed
Publication date: 2026/07/01
Hollis Jeremy AStonick Jason ATopalidou IriniYoung Janet MMoens Cecilia BLehrbach Nicolas JCampbell Melody GMalik Harmit S