NAIF1 protein{ CB12-327}rabbit.pAb
- Known as:
- NAIF1 protein{ CB12-327}host: rabbit.pAb
- Catalog number:
- 201-20-7203
- Product Quantity:
- 0.2ml
- Category:
- -
- Supplier:
- Shanghai Sunred
- Gene target:
- NAIF1 protein{ CB12-327}rabbit.pAb
Related genes to: NAIF1 protein{ CB12-327}rabbit.pAb
- Gene:
- NAIF1 NIH gene
- Name:
- nuclear apoptosis inducing factor 1
- Previous symbol:
- C9orf90
- Synonyms:
- DKFZp762G199, bA379C10.2
- Chromosome:
- 9q34.11
- Locus Type:
- gene with protein product
- Date approved:
- 2004-02-19
- Date modifiied:
- 2015-08-25
Related products to: NAIF1 protein{ CB12-327}rabbit.pAb
Related articles to: NAIF1 protein{ CB12-327}rabbit.pAb
- Radiotherapy is a primary treatment for nasopharyngeal carcinoma (NPC), yet intrinsic and acquired radioresistance limits therapeutic efficacy. Here, we investigated the functional role and mechanism of miR-1202 in NPC radioresponse. Using cell models and xenografts, we show that miR-1202 overexpression enhances cell survival, clonogenic capacity, and tumor growth following ionizing radiation (IR), whereas miR-1202 suppression produces the opposite effects. Mechanistically, miR-1202 directly targets NAIF1, leading to activation of the MAPK/ERK signaling pathway and modulation of apoptosis-related proteins, characterized by increased Bcl-2 and decreased BAX expression under IR. Notably, miR-1202 regulates ERK1/2 phosphorylation without affecting total ERK1/2 levels, indicating post-translational control of pathway activation. These findings identify a miR-1202-NAIF1-MAPK/ERK regulatory axis that contributes to NPC radioresistance and highlight miR-1202 as a potential biomarker and therapeutic target to improve radiotherapy outcomes. - Source: PubMed
Publication date: 2026/02/25
Chen XuxiaDu YouqinChen WeilingYang XiaohuiFang JingweiQu Song - - Source: PubMed
Publication date: 2021/11/16
Wu XuanxuanHu ChonglingLong ChunxiZhai XuanLiang PingYu Zengpeng - Parkinson's disease (PD) is a neurodegenerative disorder. Studies have shown that long noncoding RNA SRY-box transcription factor 2 overlapping transcript (lncRNA SOX2-OT) is highly expressed in PD patients, but its specific functions and mechanisms require further research. To address this gap, this study utilized an PD cell model induced by 1-methyl-4-phenylpyridinium (MPP). Cell viability, apoptosis, lactate dehydrogenase (LDH) activity, inflammatory factor secretion, and oxidative stress indicators were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheyltetrazolium bromide assay, LDH assay, flow cytometry, enzyme linked immunosorbent assay (ELISA), and corresponding kits, respectively. Gene and protein expression were measured using quantitative real-time-PCR and western blotting, respectively. The results indicated that microRNA-942-5p (miR-942-5p) was a direct target of lncRNA SOX2-OT and nuclear apoptosis-inducing factor 1 (NAIF1) was a direct target of miR-942-5p. The expression levels of lncRNA SOX2-OT and NAIF1 were increased, and miR-942-5p expression was decreased in SH-SY5Y cells following MPP treatment. In addition, MPP treatment reduced SH-SY5Y cell viability, increased apoptosis; increased cleaved caspase-3 protein expression and cleaved caspase-3/caspase-3 ratio; enhanced lactate dehydrogenase viability; increased tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and reactive oxygen species, and decreased superoxide dismutase activity in SH-SY5Y cells were inhibited by SOX2-OT-siRNA, and these inhibitions were reversed by miR-942-5p inhibitor. Moreover, the protective role of miR-942-5p mimic in MPP-induced SH-SY5Y cells was eliminated by the NAIF1 plasmid. Overall, lncRNA SOX2-OT-mediated regulation of oxidative stress, inflammation, and neuronal apoptosis were directly controlled by the miR-942-5p/NAIF1 signal axis in MPP-induced SH-SY5Y cells. - Source: PubMed
Guo YabiLiu YanyangWang HongLiu Peijun - Prostate cancer is the leading cause of death among men worldwide. Deregulation of microRNAs has been reported in many cancers. Expression of microRNAs miR-20a-5p, miR-21-5p, miR-100-5p, miR-125a-5p and miR-146a-5p in tissue blocks of histologically confirmed prostate cancer patients compared with BPH patients, to identify potential microRNA biomarker for prostate cancer. MicroRNA was isolated and expression was quantified by qRT-PCR using Taqman Advanced microRNA assay kits. The interactions between the microRNA:target mRNA were predicted by using bioinformatics tools such as miRwalk and miRTargetlink. The experimentally validated targets were analysed using gprofiler to identify their molecular function, biological process and related pathways. The expression analysis revealed that miR-21 and miR-100 were significantly down-regulated whereas miR-125a was up-regulated in prostate cancer patients. Comparative analysis of the expression levels with tumor grading reveal that miR-100 was significantly down-regulated (p < 0.05) in high grade tumor, indicating that miR-100 associated with prostate cancer. ROC analysis revealed that combined analysis of down-regulated miRNAs (miR-21 and miR-100) shown AUC of 0.72 (95% CI 0.65-0.79). The combined analysis of all five miRNAs showed AUC of 0.87 (95% CI 0.81-0.92). The targets prediction analysis revealed several validated targets including BCL2, ROCK1, EGFR, PTEN, MTOR, NAIF1 and VEGFA. Our results provide evidence that combined analysis of all the five miRNAs as a panel can significantly improve the prediction level of the presence of prostate cancer and may be used as a potential diagnostic biomarker. - Source: PubMed
Publication date: 2021/05/04
Damodaran MohanChinambedu Dandapani MohanapriyaSimonDuraiRaj SandhyaSundaram VenkatRamanan SRamachandran IlangovanVenkatesan Vettriselvi - The original version of this article unfortunately contained mistakes in the Affiliation section. - Source: PubMed
Wu XuanxuanHu ChonglingLong ChunxiZhai XuanLiang PingYu Zengpeng