Proteins PDGF-BB , Human
- Known as:
- Proteins PDGF-BB , Human
- Catalog number:
- C199
- Product Quantity:
- 10μg
- Category:
- -
- Supplier:
- Novoprotein
- Gene target:
- Proteins PDGF- Human
Related genes to: Proteins PDGF-BB , Human
- Gene:
- PDGFA NIH gene
- Name:
- platelet derived growth factor subunit A
- Previous symbol:
- -
- Synonyms:
- PDGF1, PDGF-A
- Chromosome:
- 7p22.3
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2016-10-05
- Gene:
- PDGFRA NIH gene
- Name:
- platelet derived growth factor receptor alpha
- Previous symbol:
- -
- Synonyms:
- CD140a, PDGFR2, GAS9
- Chromosome:
- 4q12
- Locus Type:
- gene with protein product
- Date approved:
- 1989-05-19
- Date modifiied:
- 2019-04-23
- Gene:
- PDGFRB NIH gene
- Name:
- platelet derived growth factor receptor beta
- Previous symbol:
- PDGFR
- Synonyms:
- JTK12, CD140b, PDGFR1
- Chromosome:
- 5q32
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2016-10-05
Related products to: Proteins PDGF-BB , Human
Related articles to: Proteins PDGF-BB , Human
- The clinical treatment of castration-resistant prostate cancer (CRPC) is currently a major challenge. This study explored a new combination strategy for CRPC that targeted androgen receptor (AR)-dependent and AR-independent mechanisms. First, the degradation efficiency of AR by ARV-110 was verified. CCK-8 and CellTiter-Glo assays were used to evaluate the viability of CRPC cells after treatment. The combination index of platelet-derived growth factor receptor (PDGFR) inhibitors combined with ARV-110 was calculated using CompuSyn software. Transcriptome sequencing was used to explore the in-depth mechanisms of the combination strategy. Chromatin immunoprecipitation and dual-luciferase reporter assays were used to clarify the transcriptional regulatory relationships. Coimmunoprecipitation was used to evaluate protein interactions. The results showed that ARV-110 significantly promoted AR degradation. The combination of ARV-110 and ponatinib exerted a significant inhibitory and synergistic effect on CRPC cells. The effective targets were AR and PDGFR. The combination of ARV-110 and the PDGFR-selective inhibitor JNJ10198409 effectively induced the apoptosis of CRPC cells. ARV-110 alone promoted the transcription of PDGFA. And the combination strategy further induced JNK signaling pathway activation and promoted cell apoptosis by inhibiting PDGFR activity. Additionally, the substantial accumulation of reactive oxygen species induced by the combination strategy was related to the joint downregulation of catalase by the two drugs through different mechanisms. In conclusion, this study described a new strategy for the treatment of CRPC and clarified the molecular mechanisms of the combination strategy, providing a new theoretical basis for the precision treatment of CRPC. - Source: PubMed
Publication date: 2026/04/10
Fu YangSun ShanshanLiu GuangxuDong QingzhuoWang YutaoZheng HaoyuanPiao ChiyuanBi Jianbin - Exposure to immune stress or lipopolysaccharide (LPS) during critical developmental stages like puberty may lead to gut microbiome dysbiosis and epigenetic dysregulation in mammary glands, affecting gene expression and potentially elevating breast cancer susceptibility in adulthood. Although LPS's adverse impacts on intestinal and brain functions are well-documented, its effects on mammary glands remain underexplored. Using an immunocompetent BALB/c mouse model, we administered an acute LPS dose (1.5 mg/kg body weight) during puberty. The study evaluated the long-term consequences of LPS exposure alone and combined with AHCC (Lentinula edodes cultured extract, 2 g/kg body weight/day) on DNA methylation patterns, cytokine profiles, and microRNA expression in mammary glands at 9 weeks of age. Analyses included DNA methylation sequencing, multiplex immunoassays, quantitative PCR, and image processing. Pubertal LPS exposure produced persistent molecular dysregulation in mammary glands, including differential DNA methylation (> 5% change vs. control; FDR-adjusted p < 0.05), elevated inflammatory mediators, and altered microRNA expression. Differentially methylated regions were enriched in regulatory features, with decreased methylation at transcription start sites, promoters, and 5' UTRs of genes implicated in mammary development and oncogenic signaling (including Vav3, Pdgfa, Pdgfc, Jag2, Hras, Ksr1, Il2rb, Il17b, and Il17rb) in the LPS group, whereas the AHCC + LPS group exhibited a shift toward hypermethylation at these loci (approximately 5%-10% decrease). Inflammatory profiling showed increased IL-17A/F (∼2-fold vs. control; p < 0.05), while microRNA analyses indicated reduced let-7a/c (∼30% vs. control; p < 0.05). Notably, miR-130a and miR-34a increased ∼1.5-fold across all treatment groups relative to control. Pubertal LPS exposure induces enduring epigenetic and inflammatory changes in mammary glands that may heighten breast cancer risk. AHCC's mitigating role indicates potential for dietary interventions to counteract these effects. - Source: PubMed
Yasavoli-Sharahi HamedShahbazi RoghayehAlsadi NawalSahebi Nasim BondarCuenin CyrilleCahais VincentChung Felicia Fei-LeiHerceg ZdenkoMatar Chantal - Colorectal cancer (CRC) remains one of the most important causes of cancer-related mortality worldwide, underscoring the need to better understand systemic inflammatory pathways across the colorectal neoplasia spectrum. In this exploratory case-control study, we characterized plasma levels of key inflammatory mediators in healthy individuals and patients with colorectal polyps or CRC. Healthy controls ( = 10), patients with colorectal polyps (CP, n = 16), early-onset CRC (EO-CRC, n = 11), and late-onset CRC (LO-CRC, n = 51) were prospectively enrolled. Plasma levels of sTNF-R, total TNF-α, PDGF-AA, IL-17A, and IL-1β were measured by ELISA. Group comparisons used Kruskal-Wallis tests with epsilon-squared effect sizes. PDGF-AA showed the strongest differences between controls and all neoplastic groups (ε ≥ 0.15), and these comparisons remained significant after Benjamini-Hochberg false discovery rate correction. IL-17A levels were slightly higher in EO-CRC than in LO-CRC; however, this difference did not remain significant after adjustment for multiple testing. TNF-α and IL-1β showed no significant differences across groups. Overall, this study primarily provides descriptive and hypothesis-generating evidence of differential inflammatory patterns across colorectal neoplasia, with PDGF-AA emerging as the most robust signal in this exploratory dataset. These findings do not support immediate diagnostic application and require validation in larger, prospectively recruited cohorts. - Source: PubMed
Publication date: 2026/03/13
Ionescu Vlad-AlexandruGheorghe GinaTurculet Claudiu StefanGeorgescu Teodor FlorinBratu Razvan MateiMambet CristinaEnache ValentinGheorghiu MihaelaPasarica DanielaDiaconu Camelia CristinaDiaconu Carmen CristinaBleotu Coralia - Circulating platelet-derived growth factors (PDGFs) participate in the initiation and progression of cardiovascular diseases, yet their regulatory mechanisms remain poorly understood. This study aimed to investigate the influence of air pollution on PDGFs mRNA expression in platelets and its association with acute myocardial infarction (AMI) and major adverse cardiovascular events (MACEs). We included 153 AMI patients at admission to assess the relationship between pollutant levels and platelet PDGFs mRNA expression, and followed up 142 AMI patients after discharge to evaluate the prognostic value of PDGFs mRNA expression for MACEs. Results showed that PM was non-linearly associated with the mRNA expression levels of PDGFC and PDGFD, while SO was non-linearly associated with the mRNA expression level of PDGFA (P < 0.05). Compared to controls, AMI patients at admission had an elevated PDGFD mRNA expression with a 4.705-fold increase (P < 0.05). Furthermore, PDGFs mRNA level exhibited a non-linear relationship with AMI occurrence (P < 0.05). Higher PDGFA mRNA expression (Q4 vs. Q1) was associated with a reduced risk of MACEs. Similarly, medium to high expression levels of PDGFC mRNA (Q2-Q4 vs Q1) had a lower risk of MACEs with adjusted hazard ratio of 0.366 (95% CI: 0.155-0.861). These findings indicate that exposure to air pollutants is correlated with altered PDGFs mRNA expression in platelets and that such expression is significantly associated with both AMI and subsequent MACEs. The study highlights the potential of PDGFs mRNA in platelets as a biomarker for assessing individual susceptibility to air pollution-related cardiovascular events. - Source: PubMed
Publication date: 2026/03/16
Xie JichaoXie HankunXu LiangLiu FangyuanZhuang QianZhao XianghaiSun JunxiangWei PengfeiYin YunjieChen YanchunWang FeifanHan XuLi WenWu JiahuiZhou YuanHuan ChangshengYang SongShen Chong - This study aimed to investigate the effectiveness of intraepididymal platelet-rich plasma (PRP) administration in preventing cryopreservation-induced sperm damage in rams. Twelve adult rams were randomly assigned into two groups ( = 6) in the non-breeding season. Rams in the PRP group received 0.2 ml/per epididymis (150–200 × 10⁶ platelets) of PRP every 15 days for a total of six injections, while control group received the same volume of saline. Semen samples were collected biweekly and pooled within each group before undergoing standard cryopreservation procedures. Post-thaw analyses included morphological, functional, biochemical, and molecular assessments. Compared to the control, intraepididimal PRP significantly increased hypo-osmotic swelling (HOS) response, total and progressive motility, rapid sperm percentage, and kinetic parameters (VCL- curvilinear velocity, VSL- straight-line velocity, VAP- average path velocity), while reducing static and acrosome-damaged sperm in thawed semen. Intraepididimal PRP also enhanced catalase activity, cholesterol and myristic acid (C14:0) concentrations and the levels of steroidogenic acute regulatory protein (StAR), 3β-hydroxysteroid dehydrogenase type 1 (HSD3β1), cation channel of sperm (CatSper1), platelet-derived growth factor (PDGF), platelet-derived growth factor receptor (PDGFR), and reduced malondialdehyde level in thawed semen. Intraepididimal PRP significantly increased the mRNA presence of CatSper2, CatSper3, CatSper4, transient receptor potential melastatin 3 (TRPM3) and transient receptor potential vanilloid 5 (TRPV5) ion channels, oar-miR-3958-3p and oar-miR-125b, and decreased bta-miR-22-3p and rno-miR-494 in thawed semen. Additionally, intraepididimal PRP significantly upregulated the protein expressions of CatSper3, HSD3β2 and PDGFA, and decreased protein expressions of vascular endothelial growth factor A (VEGFA) and transforming growth factor beta 1 (TGFβ1) in thawed semen. In conclusion, intraepididymal PRP administration improved cryoresistance in ram spermatozoa, likely due to its growth factors, lipids, and antioxidants. These effects enhanced post-thaw sperm quality by modulating oxidative stress, cholesterol, ion channels, microRNAs, and steroidogenic proteins, indicating PRP as a promising tool for improving semen cryotolerance in rams. - Source: PubMed
Publication date: 2026/02/28
Cinkara Serap DayanBadıllı NidaGüngör İbrahim HalilCihangiroğlu Aslıhan ÇakırAcısu Tutku CanÖzmen Görkem KırmızıkayaArkalı GözdeBulan Mustafa SezerTektemur AhmetToraman EdipKaya Şeyma ÖzerSönmez MustafaGür SeyfettinYüce AbdurraufYılmaz ÖkkeşTürk Gaffari