Titan Taq DNA Polymerase

Titan Taq DNA Polymerase is highly processive, hot stable DNA polymerase. Titan Taq DNA Polymerase has 5’ to 3’ polymerisation-dependent exonuclease replacement activity but lacks 3’ to 5’ exonuclease activity. Titan Taq DNA Polymerase with its “extendase activity” permits  TA cloning. Vrisko Limited provides a Titan Taq DNA Polymerase under product name Vrisko Titan Taq.

Vrisko Titan Taq

Applications of  Vrisko Ltd´s Titan Taq DNA Polymerase

Titan Taq DNA Polymerase is useful for a wide range of PCR assays, primer extension, and TA cloning.

Figure 1. Amplification of 420 bp target DNA using Titan Taq DNA Polymerase.
M – Vrisko Star 100 bp DNA Ladder
1-3 – Vrisko Titan Taq DNA Polymerase

Concentration: 5 units/µl

Unit definition: One unit of  Titan Taq DNA Polymerase is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74ºC.

Source: Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Quality control: Titan Taq DNA Polymerase has not nicking-, priming- , exonucleases- or unspecific endonucleases activities. SDS/PAGE – 95 kD band, >98% pure. The activity and stability have been tested. The rate of error  per nucleotide per cycle is ~2.5 x 10-5; the accuracy is ~ 4 x 104. Estimated half life at 95°C is 1.5 hours.

Storage & Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Supplied with:
10 x Reaction Buffer (Mg2+ free): 800 mM Tris-HCl pH 9.4 at 25°C, 200 mM (NH4)2SO4, 0.2% w/v Tween-20.
25 mM MgCl2

Shipping & Storage conditions: Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent. However, routine storage at -20°C is strongly recommended.

Vrisko Titan Taq is supplied for research use only.

Posted in DNA polymerase, DNA Taq polymerases, Titan Taq DNA Polymerase | Tags: DNA polymerase, PCR,Taq DNA polymerase, Titan Taq DNA Polymerase