Clinispin CT20 Centrifuge
- Known as:
- Clinispin CT20 Centrifuge
- Catalog number:
- WD4103
- Product Quantity:
- 1
- Category:
- -
- Supplier:
- Woodle
- Gene target:
- Clinispin CT20 Centrifuge
Related genes to: Clinispin CT20 Centrifuge
- Gene:
- PRSS50 NIH gene
- Name:
- serine protease 50
- Previous symbol:
- -
- Synonyms:
- TSP50, CT20
- Chromosome:
- 3p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 2009-11-06
- Date modifiied:
- 2018-01-19
Related products to: Clinispin CT20 Centrifuge
Related articles to: Clinispin CT20 Centrifuge
- The gene regulatory mechanism of individual genes during spermatogenesis is well understood in model animals, but not in non-model animals such as human. The mouse Prss/Tessp locus encompassing six testis-specific protease genes is a model of gene function and regulation in the testis. Here we investigated the expression and regulation of PRSS locus in primates, especially human. Unlike high expression of all six genes in mouse testis, one or two different PRSS genes were predominantly expressed in six primate species. In human testis, PRSS50 was a predominant gene, and the other five PRSS genes were expressed at low levels. Interestingly, promoters of four lowly expressed PRSS genes were marked with both histone H3K4 trimethylation and histone H3K27 trimethylation in human spermatocytes and spermatids, while their orthologous mouse genes were marked only with histone H3K4 trimethylation. Additionally, an ATAC-seq peak in human testis exhibited silencer activity for PRSS45 which is a lowly expressed gene in human but a predominant gene in chimpanzee. These results suggest that bivalent chromatins and an active silencer cooperatively function to achieve appropriate levels of expression for individual PRSS genes during human spermatogenesis. These findings provide mechanistic insights into gene regulation in human spermatogenesis. - Source: PubMed
Iijima KoutaShibayama RyuzaburoFujimori ChikaKimura Atsushi P - Accessory breasts denote the formation of extra breast tissue along the milk line, and are known to be more prevalent among Black and Asian populations, affecting both genders. This first-ever study aimed to determine the genetic aetiology of accessory breasts in a multiplex family, where all female siblings present with bilateral accessory breasts. The study also ascertained secondary findings (SFs) responsible for comorbidities. Clinical data and saliva samples were obtained from all family members. Ultrasound and histopathology confirmed the diagnosis. Whole-exome sequencing was conducted on DNA samples obtained from the saliva, with variant calling conducted utilising the Sentieon workflow. Variant classification was based on American College of Medical Genetics and Genomics guidelines. After segregation analysis, 12 candidate genes emerged. Among these, and emerged as top candidates, being implicated in breast diseases. However, two variants in (c.360del; p.His120GlnfsTer24 and c.355_358del; p.Ser119IlefsTer24) were selected as the most probable causal variants because of the role of this gene in hereditary breast and ovarian cancer syndromes. The remaining ten genes were reported as potentially accounting for comorbidities segregating with accessory breasts. Reported SFs involve and . In conclusion, pathogenic variants in cause familial accessory breasts. These novel observations impact pathophysiology, genetic counselling, and personalised medicine. - Source: PubMed
Publication date: 2025/10/22
Danbaki Abass ShaibuAsamoah Christian OpokuMensah Gideon OkyereTsri BruceBusch Tamara DArthur Fareed Kow NanseKyei IshmaelBlay Lawrence KobinaMensah SamuelAdeyemo Adebowale AButali AzeezDonkor PeterGowans Lord Jephthah Joojo - Serine protease 50 (PRSS50/TSP50) is highly expressed in spermatocytes. Our study investigated its role in testicular development and spermatogenesis. Initially, PRSS50 knockdown was observed to impair DNA synthesis in spermatocytes. To further explore this, we generated PRSS50 knockout ( ) mice ( ), which exhibited abnormal spermatid nuclear compression and reduced male fertility. Furthermore, dysplastic seminiferous tubules and decreased sex hormones were observed in 4-week-old mice, accompanied by meiotic progression defects and increased apoptosis of spermatogenic cells. Mechanistic analysis indicated that PRSS50 deletion resulted in increased phosphorylation of extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and elevated levels of MAP kinase phosphatase 3 (MKP3), a specific ERK antagonist, potentially accounting for testicular dysplasia in adolescent mice. Taken together, these findings suggest that PRSS50 plays an important role in testicular development and spermatogenesis, with the MKP3/ERK signaling pathway playing a significant role in this process. - Source: PubMed
Niu Chun-XueLi Jia-WeiLi Xiao-LiZhang Lin-LinLang YanSong Zhen-BoYu Chun-LeiYang Xiao-GuangZhao Hai-FengSun Jia-LingZheng Li-HuaWang XueSun YingHan Xiao-HongWang Guan-NanBao Yong-Li - Diabetes is characterized by persistently high blood glucose levels and severe complications and affects millions of people worldwide. In this study, we explored the epigenetic landscape of diabetes using data from the Korean Genome and Epidemiology Study (KoGES), specifically the Ansung-Ansan (AS-AS) cohort. Using epigenome-wide association studies, we investigated DNA methylation patterns in patients with type 2 diabetes mellitus (T2DM) and those with normal glucose regulation. Differential methylation analysis revealed 106 differentially methylated probes (DMPs), with the 10 top DMPs prominently associated with TXNIP, PDK4, NBPF20, ARRDC4, UFM1, PFKFB2, C7orf50, and ABCG1, indicating significant changes in methylation. Correlation analysis highlighted the association between the leading DMPs (e.g., cg19693031 and cg26974062 for TXNIP and cg26823705 for NBPF20) and key glycemic markers (fasting plasma glucose and hemoglobin A1c), confirming their relevance in T2DM. Moreover, we identified 62 significantly differentially methylated regions (DMRs) spanning 61 genes. A DMR associated with PDE1C showed hypermethylation, whereas DMRs associated with DIP2C, FLJ90757, PRSS50, and TDRD9 showed hypomethylation. PDE1C and TDRD9 showed a strong positive correlation between the CpG sites included in each DMR, which have previously been implicated in T2DM-related processes. This study contributes to the understanding of epigenetic modifications in T2DM. These valuable insights can be utilized in identifying potential biomarkers and therapeutic targets for effective management and prevention of diabetes. - Source: PubMed
Publication date: 2023/12/13
Seo HyeinPark Jae-HoHwang Jin-TaekChoi Hyo-KyoungPark Soo-HyunLee Jangho - (1) Background: Epigenome-wide association studies (EWAS) in peripheral blood have repeatedly found associations between tobacco smoking and aberrant DNA methylation (DNAm), but little is known about DNAm signatures of smoking in the human brain, which may contribute to the pathophysiology of addictive behavior observed in chronic smokers. (2) Methods: We investigated the similarity of DNAm signatures in matched blood and postmortem brain samples ( = 10). In addition, we performed EWASs in five brain regions belonging to the neurocircuitry of addiction: anterior cingulate cortex (ACC), Brodmann Area 9, caudate nucleus, putamen, and ventral striatum ( = 38-72). (3) Results: cg15925993 within the gene was epigenome-wide significant in the ACC. Of 16 identified differentially methylated regions, two ( and ) overlapped between multiple brain regions. Functional enrichment was detected for biological processes related to neuronal development, inflammatory signaling and immune cell migration. Additionally, our results indicate the association of the well-known CpG site cg05575921 with smoking in the brain. (4) Conclusion: The present study provides further evidence of the strong relationship between aberrant DNAm and smoking. - Source: PubMed
Publication date: 2022/04/02
Zillich LeaPoisel EricStreit FabianFrank JosefFries Gabriel RFoo Jerome CFriske Marion MSirignano LeaHansson Anita CNöthen Markus MWitt Stephanie HWalss-Bass ConsueloSpanagel RainerRietschel Marcella