NME4, 33-187aa, Recombinant Protein
- Known as:
- NME4, 33-187aa, Recombinant Protein
- Catalog number:
- GWB-P0992D
- Product Quantity:
- 500ug
- Category:
- -
- Supplier:
- GenWay
- Gene target:
- NME4 33-187aa Recombinant Protein
Related genes to: NME4, 33-187aa, Recombinant Protein
- Gene:
- NME4 NIH gene
- Name:
- NME/NM23 nucleoside diphosphate kinase 4
- Previous symbol:
- -
- Synonyms:
- nm23-H4, NM23H4, NDPKD
- Chromosome:
- 16p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1996-12-19
- Date modifiied:
- 2016-07-15
Related products to: NME4, 33-187aa, Recombinant Protein
Related articles to: NME4, 33-187aa, Recombinant Protein
- Vitrification is a vital tool for the long-term preservation of animal genetic resources, yet cryoinjury-manifesting as oxidative stress, structural damage, and metabolic disorders-severely compromises its efficacy. Here, we investigated the protective effects of melatonin (MT) supplementation on the cryotolerance of mouse morulae. First, mouse morulae were assigned to four groups treated with vitrification and thawing media containing MT (0, 10, 10, and 10 M) to determine optimal MT concentration. Subsequently, embryos treated with the optimal MT concentration were evaluated for developmental competence, oxidative stress, apoptosis, and mitochondrial function. Furthermore, transcriptome sequencing was performed to elucidate MT-regulated molecular pathways. The results demonstrated that MT supplementation at 10 M significantly enhanced developmental competence, as evidenced by increased blastocyst rate, hatched blastocyst rate, total cell number and the inner cell mass (ICM)-to-total cell ratio compared to the MT-free group ( < 0.05). Consequently, embryo transfer outcomes showed higher live births and weaned pups in the 10 M MT group versus those in controls ( < 0.05), achieving levels comparable to fresh embryos ( > 0.05). Mechanistically, MT reversed cryoinjury-induced mitochondrial dysfunction by elevating membrane potential(MMP) and Adenosine Triphosphate(ATP) production while reducing Reactive Oxygen Species (ROS) accumulation ( < 0.05). Transcriptomic analysis further revealed that vitrification perturbed metabolic pathways, including amino acid/fatty acid degradation and glucose/pyruvate metabolism. MT downregulated cryoinjury-induced overexpression of and , inhibiting excessive NF-κB activation and alleviating metabolic dysfunction. Additionally, MT restored expression of nucleotide synthesis genes (, , , , , ) critical for cell proliferation, and reversed downregulation of mitochondrial genes and , confirming restoration of mitochondrial homeostasis. In conclusion, melatonin alleviates vitrification-induced cryoinjury by restoring mitochondrial function, which rescues nucleotide synthesis and partially reverses associated metabolic dysfunction. These findings advance MT-mediated cryoprotection and underscore its translational value for embryo cryopreservation in animal genetic resource conservation. - Source: PubMed
Publication date: 2026/05/26
Ji PengyunMa WenkuiZhao MengmengYan LaiqingLiu YunjieYin DepengChen QianruChen BodaWu HaoGao ShuaiWang BingyuanZhang LuLiu Guoshi - Minimising methane (CH) emissions from livestock production is a global priority, and feed modifications, such as supplementing diets with microalgae, have previously been shown to help reducing enteric CH production. This study explored blood-derived host gene expression profiles from twenty lambs supplemented with increasing levels of microalgae oil to investigate their transcriptional responses associated with varying microalgae oil levels while also exploring the host systemic responses towards varied CH productions. - Source: PubMed
Publication date: 2026/06/23
Chacko Kaitholil Steffimol RoseMooney Mark HAubry AurélieCristobal-Carballo OmarHillis RichardRazban VahidYan TianhaiRezwan Faisal IMorrison StevenHuws SharonShirali Masoud - Gastric cancer (GC) exhibits marked epidemiological differences between European (EUR) and East Asian (EAS) populations, with significantly higher incidence rates in EAS. Circulating proteins represent promising drug targets; however, most proteomic studies have focused primarily on EUR ancestry, leaving EAS-specific targets largely underexplored. This study aims to identify ancestry-specific plasma protein targets for GC using Mendelian randomization (MR). - Source: PubMed
Publication date: 2026/05/01
Zhi PengCui YanXue GuanghuiQiao LingyuGeng JieChang ZhengyaoXu YinmeiYan JuanjuanWang YingliZhao Chenghui - The development of circularly polarized luminescence (CPL) emitters with a luminescence dissymmetry factor (|g|) approaching the theoretical limit of 2 remains a central challenge in chiral photonics. Herein, considerable g values (1.48-1.65) were achieved in helicates (NMe)[Eu(R/S-)] (ΔΔ/ΛΛ--) with different structural rigidity, as well as in crown ether-modified (NMe)[Eu(R/S-)] (ΔΔ/ΛΛ-) that exhibits adjustable coordination geometry symmetry. Notably, encapsulating bulkier [NEt] instead of a [NMe] counterion within the inner cavity of ΔΔ/ΛΛ- further elevates |g| to an unprecedented 1.71, the highest value for any chiral molecular emitter. While the NMe-ΔΔ/ΛΛ- achieves an ultrahigh CPL brightness (B = 3625 M cm), resulting from its large g value (1.63) and high quantum yield (Φ = 32%). Structural and spectroscopic analyses demonstrate that the high CPL activity originates from a conformationally rigidified square antiprismatic (SAP) geometry around the Eu(III) center. This structure and property relationship is vividly demonstrated by the crown-ether-functionalized ΔΔ-, where gradual binding of Cs ions triggers a pronounced g fluctuation (1.48 → 0.62 → 1.41) through a perturbation and subsequent restoration of the SAP environment. Furthermore, the multicrown-ether binding sites in ΔΔ- enable cooperative guest binding, facilitating the first naked-eye CPL recognition of the antispasmodic drug tizanidine. This work establishes rigidifying SAP configuration as a general design principle for maximizing the CPL activity of lanthanide complexes and opening avenues for advanced CPL applications. - Source: PubMed
Publication date: 2026/04/15
Huang WenruYao ZhiweiYin SenGao TingCrassous JeanneZhou YanyanLi Hongfeng - This study aimed to comprehensively characterize the relationship between glycated hemoglobin (HbA1c) and intervertebral disc degeneration (IVDD) by integrating population-level epidemiological profiling, Mendelian randomization (MR) analysis, multi-omics data, and single-cell transcriptomics. The objectives included clarifying the causal effect of HbA1c on IVDD, identifying key HbA1c-related genes, elucidating their cellular and molecular roles in disc degeneration, and exploring potential therapeutic targets. Global Burden of Disease (GBD) 2021 database was used to evaluate the incidence rate of diabetes mellitus (DM) and low back pain (LBP) across the entire global population. To assess the causal effect of HbA1c on IVDD, MR approache was conducted using summary statistics from large genome-wide association studies. Subsequently, integrating cis-eQTL information, bulk RNA sequencing, and single-cell RNA sequencing were performed to identify HbA1c-related genes involved in disc degeneration, and explore their roles along pseudotime differentiation trajectories. Potential associations between hub genes and IVDD, as well as candidate therapeutic compounds targeting these genes, were further evaluated through database screening and molecular docking. The findings from GBD indicated a correlation between diabetes and low back pain in both 1990 and 2021. The results of MR analysis revealed a causal relationship between HbA1c levels and IVDD. After integrated analysis of the eQTLGen database and RNA-seq data, we identified five hub HRGs, including STAT5A, CDKN1C, TRPS1, NME4, and OASL, which have been implicated in multiple biological processes and pathways associated with IVDD. Moreover, the scRNA-seq analysis showed that hub HRGs were associated with specific cell subpopulations in human nucleus pulposus (NP) tissues, such as inflamed chondrocytes, fibrochondrocytes, chondrocytes, and calcifying chondrocytes. Additionally, hub HbA1c related genes (HRGs) have been found to be associated with several DDDs such as back pain, osteoporosis, and spinal stenosis. Meanwhile, 12 drugs, including resveratrol, dexamethasone, and simvastatin, were identified that have the potential to target hub HRGs. This study revealed a causal effect of HbA1c on IVDD and identified five key HbA1c-related genes (STAT5A, CDKN1C, TRPS1, NME4, and OASL) involved in disc degeneration, providing potential targets for future research. - Source: PubMed
Publication date: 2026/03/27
Li ZhonghuiMa WeibangSun LingzhiGuo JiajieZhuang YongNing XuSun HongLiu Miao