Mouse R-Spondin 1 / RSPO1 Protein
- Known as:
- Mouse R-Spondin 1 / RSPO1 Protein
- Catalog number:
- RS1-M5220
- Product Quantity:
- 1mg
- Category:
- -
- Supplier:
- acrobyosystems
- Gene target:
- Mouse R-Spondin 1 / RSPO1 Protein
Related genes to: Mouse R-Spondin 1 / RSPO1 Protein
- Gene:
- RSPO1 NIH gene
- Name:
- R-spondin 1
- Previous symbol:
- -
- Synonyms:
- FLJ40906, RSPONDIN
- Chromosome:
- 1p34.3
- Locus Type:
- gene with protein product
- Date approved:
- 2005-08-08
- Date modifiied:
- 2016-10-05
Related products to: Mouse R-Spondin 1 / RSPO1 Protein
Related articles to: Mouse R-Spondin 1 / RSPO1 Protein
- Intestinal organoids are widely used in mammalian studies to mimic and study intestinal function and host-pathogen interactions without using of whole animal studies. For chicken, this was not yet feasible. Recently, we have shown that embryonal intestinal organoids can grow for 10-15 passages (approximately 6 weeks) when using chicken-derived Rspo1 and Wnt3. In this paper, we show that replacing Wnt3 with chicken Wnt3a and adding chicken Nrg1 and human FGF1 results in efficient and long lasting growth, over 40 weeks, of embryonal chicken intestinal organoids. Moreover, the typical branching phenotype, which is normally observed for mammalian species such as mouse and pig intestinal organoids, is now also observed in the chicken organoids when Rspo1, Wnt3a, Nrg1 and hFGF1 are used. This branching is enhanced by inhibiting FoxO1 with AS1842856, resulting in an increase of growth. Moreover, we show the presence of Lgr5 positive stem cells, goblets, endocrine cells, Paneth cells and enterocytes. In agreement with the observed presence of goblet cells, we observed mucus secretion in the 2D cultures. This new and improved culture condition for chicken intestinal organoids allows for in vitro research that can partially replace animal testing with respect to, for instance, replacement of antibiotics, feed additives and drug development. - Source: PubMed
Publication date: 2026/06/24
Klooster Jean Paul Tenvan Summeren Kittyde Louwere JasperVelkers Francisca CPieters Raymond H H - The RING finger (RNF) protein family, the largest family of E3 ubiquitin ligases, plays a critical role in tumorigenesis and progression; however, a comprehensive macroscopic analysis of this field is still lacking. Colorectal cancer (CRC) is a highly prevalent malignancy worldwide, and its substantial heterogeneity urgently calls for more precise prognostic biomarkers. This study integrated bibliometric and bioinformatics approaches to systematically delineate the RNF research landscape, construct a CRC prognostic risk model based on the RNF43 interaction network, and explore its association with the tumor microenvironment (TME). Bibliometric analysis revealed that publications in the RNF field have increased rapidly (compound annual growth rate of 28.5%), with research hotspots shifting toward CRC and other diseases, and RNF43 emerging as a core frontier. Analysis of TCGA and GTEx data showed that RNF43 was significantly overexpressed in CRC. Through the STRING database, RNF43-interacting proteins were screened and intersected with CRC-related targets, identifying RNF43-mediated core CRC targets (RNF43, FZD1, FZD5, FZD8, LGR4, LGR5, RSPO1, RSPO2, RSPO3, RSPO4, and AKAP8L). Based on the TCGA-COAD+READ cohort (n = 541), these core targets were incorporated into LASSO regression analysis, resulting in a risk score model comprising LGR4, RSPO4, AKAP8L, and RNF43. In the training set (TCGA-COAD+READ), the high-risk group exhibited significantly shorter overall survival (OS), with AUCs of 0.61, 0.60, and 0.61 for 1-, 3-, and 5-year OS, respectively. The model was externally validated in the GSE39582 cohort (n = 579), although the AUC values remained modest. Multivariate Cox regression analysis demonstrated that the risk score, age, and tumor stage were independent prognostic factors for OS in CRC patients; a nomogram integrating these three factors showed acceptable calibration. TME analysis revealed that the high-risk group displayed a "pro-inflammatory phenotype under immunosuppression": increased infiltration of stromal components, increased CD8⁺ T cells but decreased CD4⁺ memory T cells, unexpectedly elevated M1 macrophages, and no significant difference in overall immune score. This complex imbalance pattern-characterized by increased effector cells, loss of memory cells, and pro-inflammatory polarization accompanied by potential functional suppression-suggests a possible mechanism of immune evasion. In conclusion, this study is the first to adopt a strategy combining "macro-level bibliometric insights" with "micro-level bioinformatics mining," systematically delineating the research trajectory of the RNF family and successfully constructing and validating a CRC prognostic risk model based on the RNF43 interaction network. This model may serve as an auxiliary stratification tool with potential prognostic value and may also reveal associations between the risk score and TME stromal activation and immune dysfunction, providing new insights for precise prognostic assessment and individualized treatment of CRC. - Source: PubMed
Publication date: 2026/08/21
Song JiayuZhang HuanhuanZheng ZelinShi RanranZhang Qingwei - is an economically important amphibian in Northeast China, valued for its medicinal oviduct (). However, the low proportion of females in artificial culture severely restricts industrial productivity. To elucidate the molecular mechanisms of hormone-induced sex reversal, based on our previous research results, we exposed tadpoles to 17β-estradiol (E) or testosterone propionate (TP). We then monitored gonadal histology, conducted multi-stage transcriptome sequencing, and validated key genes via qRT-PCR. The results show that induction treatment with 40 μg·L E and 80 μg·L TP resulted in 93.33% female and 100% male populations, respectively. Both hormones promoted germ cell proliferation at the undifferentiated stage and directed stable ovarian or testicular development without intersex abnormalities. Ovarian differentiation involved progressive multi-wave transcriptional reprogramming, whereas testicular differentiation exhibited a concentrated gene expression burst during gonadal maturation. Steroid hormone biosynthesis, Wnt, and PPAR pathways formed the core regulatory network. Three female-biased genes (, , ) and three male-biased genes (, , ) were identified, with their expression dynamics aligned with histological progression. This study defines the optimal parameters and molecular signatures of bidirectional sex reversal in . It provides a practical foundation for sex-control breeding and offers amphibian-based evidence for the conservation and plasticity of vertebrate sex determination. - Source: PubMed
Publication date: 2026/08/06
Fu HualinSun YanqiuSun YiwenMeng XiangyuXu WeiXu YuanDu Zhiheng - Broader research and translational use of patient-derived organoids (PDOs) in pancreatic ductal adenocarcinoma (PDAC) remain constrained by complex and costly culture requirements. Here, we developed a conditional transgenic strategy to generate self-sustaining primary PDAC organoids and explored its feasibility for therapeutic evaluation. - Source: PubMed
Publication date: 2026/07/24
Wang XuWang RuijieLin XuanZou XuanChen YushengMa MingjianPan MengxingChen JinxinYan YuDing FeiZhou ShuaiYu XianjunLiu ChenWang XuCheng He - The molecular architecture underlying diverse vertebrate sex-determining systems remains elusive despite fragmentary evidence of changes in upstream regulators and downstream mediators. Here we modeled species-specific regulatory networks of urogonadal development for turtles with contrasting mechanisms [Apalone spinifera - ZZ/ZW genotypic sex determination (GSD), and Chrysemys picta - temperature-dependent sex determination (TSD)] using matched data from time-course sampling. We uncovered key steps in the evolutionary transition of sex determination by testing for conservation or divergence of network modular components. Specifically, we tested these alternative hypotheses: first, transcription factor (TF) hubs and their target genes are conserved between species (null H0); second, the same TF hub acquired a new set of target genes in a species, retaining or not ancestral functions (H1 and variants); third, a new TF hub took over the regulation of the former gene targets of an ancestral TF (H2); and finally, complete overhaul occured where both ancestral TF hubs and their target genes were replaced in a species (H3). Results implicate primary cilia as integrators of environmental signals underlying TSD, because known thermosensitive TSD components (e.g., calcium-redox, pSTAT3, Wnt/Rspo1/β-catenin, Dhh) overrepresented in our results are linked to primary cilia. TFs that evolved between species also regulate primary cilia and point to key changes in their sensory machinery that accompanied TSD-GSD transitions (e.g., calcium/ion channels or membrane transport components in Chrysemys versus structural elements and ciliogenesis in Apalone). This novel Primary Cilia Integration hypothesis expands current models of epigenetic regulation of turtle sexual development, the evolution of plasticity versus canalization, and warrants functional validation. - Source: PubMed
Publication date: 2026/07/29
Gessler Thea BAdams Dean CValenzuela Nicole