Midori Green Advance
- Known as:
- Midori Green Advance
- Catalog number:
- MG03
- Product Quantity:
- 50
- Category:
- -
- Supplier:
- Other suppliers
- Gene target:
- Midori Green Advance
Related genes to: Midori Green Advance
- Gene:
- ALPK3 NIH gene
- Name:
- alpha kinase 3
- Previous symbol:
- -
- Synonyms:
- MAK, KIAA1330, Midori
- Chromosome:
- 15q25.3
- Locus Type:
- gene with protein product
- Date approved:
- 2004-12-01
- Date modifiied:
- 2016-10-05
Related products to: Midori Green Advance
Related articles to: Midori Green Advance
- Heart specialization involves nuclear programs however, chamber-specific regulation of the nuclear proteome landscape remains unknown. In this study we isolated the nucleus from four major anatomical regions of healthy mouse heart (fresh) and employed quantitative mass spectrometry-based proteomics to construct a comprehensive nuclear proteome landscape of left ventricle (LV, 2403 proteins), right ventricle (RV, 2242 proteins), left atrium (LA, 2368 proteins), right atrium (RA, 1816 proteins). This led to the discovery of nuclear regional proteome signatures [ventricular signature, 297 proteins; atrial signature, 183 proteins]; associated with oxidative metabolism and redox regulation, ferroptosis, extracellular-matrix remodeling, SUMO- and stress-responsive control and transcriptional regulation. Chamber-level analyses further identify distinct nuclear features in LV (120 proteins), LA (188 proteins), and RA (72 proteins). In addition, we defined conserved core nuclear proteome (230 proteins) shared across all anatomical regions, enriched for transcription-regulator complexes, nucleolar/ribosome-associated, RNA-processing, and chromatin-organization components. Within this core network we report 78 transcription factors/co-factors and select nuclear, chromatin and RNA export-associated proteins including 29 specific factors (e.g., Alpk3, Rbm14, Arglu1, Hmgb1, Myef2, Sf1) associated with the heart. Regionally, we verified spatial localization in heart of H2ac21 and Sun2 in LA and Ptbp2 in LV by immunofluorescence. This study provides insights into the chamber-resolved view of the nuclear proteome in the heart, establishes a framework for linking nuclear proteomic signatures to atrial and ventricular biology, unique features of the heart nuclear proteome landscape relative to other organs, and a baseline for studying nuclear remodeling in cardiac pathophysiology. - Source: PubMed
Publication date: 2026/05/14
Eslami Seyed SadeghRai AlinFang HaoyunThomas AnitaCross JonathonDonner DanielGreening David W - Equus caballus is a species of considerable economic and cultural significance. However, the regulatory networks involved in equine sexual maturation remain unclear and eventually limit its reproduction and utilization. In this study, testicular tissues from eight Kazakh horses at two developmental stages (2 years, representing pre-maturation, and 3 years, representing post-maturation) were analyzed using whole transcriptome sequencing, data-independent acquisition (DIA) proteomics, and untargeted metabolomics. An integrated regulatory network was constructed encompassing ceRNA, mRNA, protein, and metabolite interactions. Transcriptome analysis revealed that significantly changes circRNAs, LncRNAs, including eca-miR-34-5p, eca-miR-34c, eca-miR-449a, eca-miR-486-3p, and target genes such as TRAF1, TRAF3, ALPK3, SLC29A4 and CPNE3. A total of 277 differentially expressed genes were identified at both the mRNA and protein levels, which are primarily involved in biological processes including PI3K-AKT signaling, focal adhesion, extracellular matrix (ECM) receptor interactions, phospholipase D signaling, and ovarian steroidogenesis. LncRNAs and circRNA were found to modulate COL1A1 expression by competitively binding to miR-7844-x and regulating SPARC through interactions with eca-miR-146-5p and eca-miR-328. Integrated proteomic and metabolomic analyses highlighted TAP1, COL14A1, PRDX3, and SEBENP1 central proteins, along with key metabolites such as guanosine, Austocystin B, methanone, and glycerophosphocholine. These molecules were mainly enriched in pathways related to ovarian steroidogenesis, steroid hormone biosynthesis, and propionate metabolism. In conclusion, this study presents a comprehensive regulatory network involving LncRNA, circRNA, miRNA, mRNA, proteins, and metabolites during equine testicular development. The findings provide novel insights into the molecular basis of equine sexual maturation and offer a valuable foundation for improving reproductive regulation and breeding efficiency in horses. - Source: PubMed
Publication date: 2026/04/02
Yang XixiWen LiuxiangWen MingyueYao XinkuiMeng JunZeng YaqiRen Wanlu - Hypertrophic cardiomyopathy (HCM) is a common inherited disease and a leading known cause of sudden cardiac arrest in young adults and athletes. While genetic testing has advanced rapidly in the past decade, the yield of genetic testing remains low. The Clinical Genome Resource (ClinGen) initiative has become a leading resource for defining the clinical relevance of genetic variants with expert groups focusing on evaluating the strength of evidence for each HCM implicated gene. With the rise of large biobanks and population databases, genetic discovery has been significantly advanced. However, whether these databases can be used to validate gene-disease associations curated by ClinGen and provide evidence for novel gene-disease associations remains unclear. Here, we utilized a publicly available database containing 748,879 individuals across three large biobanks (All of Us, UK biobank, Mass General Brigham biobank). We tested the association of rare coding variants in each gene in the HCM ClinGen panel with HCM. In total, 38 genes were tested, and Bonferroni correction was applied accordingly. Of the 12 genes with definitive evidence for HCM (e.g., , , , ), 8 (67%) demonstrated nominally significant association with HCM on a population level, and 5 (42%) remained significant after Bonferroni correction, further supporting the validity of these genes in HCM panels. Several definitive genes which are much less commonly affected in HCM (, , , , , , and ) did not pass our Bonferroni corrected-significance threshold, but all had positively associated effect sizes with HCM. No genes deemed to have moderate or limited evidence had any significant associations with HCM even before Bonferroni correction. Altogether, we show that large biobanks and population databases generally recapitulate established gene-disease associations for HCM and support the ClinGen group's gene curations. The utilization of such publicly accessible databases represents an additional tool for assessing gene validity in monogenic cardiac disorders with an established phenotype, although it may have limited sensitivity and should not be solely relied on. - Source: PubMed
Publication date: 2026/03/21
Dababneh Saif FOng KevinYeung DarwinHawkins Nathaniel MKrahn AndrewLaksman ZacharyTadros RafikRoston Thomas M - To explore the clinical and genetic characteristics of a Chinese pedigree affected with Hypertrophic cardiomyopathy (HCM) due to a truncating variant of ALPK3 gene. - Source: PubMed
Hong ChenliangDing XianhongLu YangZhu JiaWang JinweiXu MengyiChen ShuaishuaiShen BoGe Weili - Hypertrophic cardiomyopathy (HCM) is an autosomal dominant cardiovascular disease characterised by myocardial hypertrophy with a prevalence of approximately 0.2%-0.5%. Recently, in addition to mutations in genes encoding sarcomeric proteins, which have traditionally been implicated in the development of HCM, mutations in genes encoding non-sarcomeric proteins have also been found to be associated with the development of HCM.This report details the first documented case in China of severe HCM caused by compound heterozygous mutations in the non-sarcomeric proteins () gene. - Source: PubMed
Publication date: 2025/12/08
Liu Wen-JingHua YangJiao Feng-HuiHu Hai-YingXu Wen-JuanWang Ya-NanDuan Li-PingZhao Xiu-FengZhang Ren-JieChang Chao