ELISA Human , TNF-alpha
- Known as:
- Enzyme-linked immunosorbent assay test Human , TNF-a
- Catalog number:
- RBMS223/4R
- Product Quantity:
- 96 wells (1 kit)
- Category:
- -
- Supplier:
- Biovend
- Gene target:
- ELISA Human TNF-alpha
Related products to: ELISA Human , TNF-alpha
Related articles to: ELISA Human , TNF-alpha
- Septic arthritis is a severe infection caused by bacteria directly invading the synovial joints. It leads to inflammatory damage to articular cartilage and may result in significant functional impairment, with a high recurrence rate. The rapid spread of antibiotic-resistant bacterial strains has further exacerbated the threat to human health, particularly in septic arthritis cases. Based on the antibacterial activity and regenerative properties of pancreatitis associated protein (PAP), this study aims to investigate the therapeutic potential of exogenous recombinant PAP for treating methicillin-resistant Staphylococcus aureus (MRSA)-induced septic arthritis. The prepared PAP exhibited favorable bactericidal effects against both Staphylococcus aureus (S. aureus) and MRSA, with a dose-dependent manner. The antibacterial efficacy in the high-dose group was comparable to that of vancomycin. Additionally, 10 nM PAP significantly promoted the proliferation of RAW264.7 macrophages and enhanced their resistance to oxidative stress. Intra-articular injection of PAP accelerated the recovery from MRSA-induced septic arthritis by alleviating joint swelling, clearing the bacteria within the joint cavity, and mitigating inflammation. Transcriptomic results indicated that PAP administration significantly downregulated the expression of inflammatory markers and slightly increased the proportion of monocytes/macrophages. Further studies on the immunomodulatory effects of PAP revealed that it attenuated lipopolysaccharide (LPS)-induced M1 polarization and markedly diminished the expression of pro-inflammatory cytokines, including TNF-α, IFN-γ, and IL-6. Meanwhile, PAP partially induced M2 polarization of macrophages and elevated anti-inflammatory IL-4 levels. In conclusion, PAP exhibited antibacterial, tissue repair, and immunomodulatory activities, highlighting its potential as a therapeutic candidate for septic arthritis. - Source: PubMed
Publication date: 2026/05/08
Yu LutingLin GuanghaoJiang ShenglongLi YuanxinRen LiliChen Guoguang - The safety of live probiotics remains debated due to concerns regarding their viability and functional efficacy under harsh in vivo and in vitro conditions. Inflammatory bowel disease (IBD) is a complex disease stemming from various factors. It has been reported that probiotics shows anti-inflammatory function. This study evaluated the immunomodulatory and anti-inflammatory properties of heat-killed Lactobacillus strains (a form of postbiotics). Because viable Loigolactobacillus coryniformis NA-3 exhibited lower in vitro viability than Lactiplantibacillus plantarum NA, we first evaluated the phagocytic activity and TLR2-mediated immunoregulatory effects of heat-killed L. coryniformis NA-3 in RAW 264.7 cells, and conducted a preliminary comparative assessment of both live and heat-killed L. coryniformis NA-3 in murine models. We further investigated two distinct heat-killed strains: L. coryniformis NA-3 and Lactiplantibacillus plantarum NA. Heat-killed L. coryniformis NA-3 induced TLR2-dependent immunomodulatory effects in vitro and showed effects comparable to those of its live counterpart in vivo under the conditions tested. Both heat-killed strains were associated with changes in inflammatory markers, including decreased levels of pro-inflammatory cytokines (IL-6, TNF-α) and increased levels of anti-inflammatory mediators (IL-4, IL-10, TGF-β) in mice. In addition, they were associated with alterations in antioxidant parameters, including changes in SOD, GSH-Px, GR, NQO1, HO-1, Nrf2, and MDA levels, as well as increased expression of the tight junction proteins claudin-1 and occluding. Observations from this study are consistent with the potential involvement of the TLR2/NF-κB p65 signaling pathway. These findings highlight the potential of heat-killed probiotics as safe and effective alternatives to live strains in immune regulation and anti-inflammation. - Source: PubMed
Publication date: 2026/05/08
Xu XiaoqingTian DandanQiao YuPeng QingRan ChaoYang YalinZhang ZhenYao YuanyuanDing QianwenZhou Zhigang - To mitigate bacterial infections in aquaculture caused by antibiotic overuse, two highly effective antimicrobial peptides, AS-CATH4 and ALFPm3, were introduced into turbot aquaculture in this study. A microalgal chloroplast expression system was established in Tetraselmis subcordiformis through tandem expression of these two exogenous antimicrobial peptides. This system was then used to produce microalgal feed for turbot (Scophthalmus maximus L.). The in vitro antimicrobial capacity and in vivo functional effects on turbot were evaluated. The results indicated that the engineered microalgal strain (ASD) containing the two antimicrobial peptides stably expressed these peptides and exhibited inhibitory effects against Staphylococcus aureus, Vibrio parahaemolyticus, and V. splendens. Feeding turbot with commercial feed mixed with ASD downregulated inflammatory factors such as TNF-α, IL-1β, and IL-8R in the gut and liver of turbot, thereby modulating inflammatory responses. Simultaneously, this treatment modulated the dynamic equilibrium of the gut microbiota by remodeling the abundance and diversity of gut bacteria. In conclusion, this study provides strong evidence for the use of microalgae oral delivery systems to deliver antimicrobial peptides, thereby enhancing the immunity of aquatic organisms and regulating the function of their gut microbiota. - Source: PubMed
Publication date: 2026/05/08
Liu YunyunJie XinhuiWang JiaqingHan SubingLiu PingMeng ChunxiaoWang HuiGao ZhengquanCui Yulin - Chronic non-infectious uveitis can lead to vision loss if not adequately controlled. Thus, steroid-sparing immunomodulatory therapy (IMT) is often required to achieve and maintain disease quiescence. It is unclear if biologic agents inhibiting tumor necrosis factor alpha (TNF-α) provide superior control of chronic uveitis compared to conventional IMTs on their own or as combination therapy. - Source: PubMed
Publication date: 2026/05/08
Zhang CharlesMcSoley MatthewErsan SinanYeh StevenAlbini Thomas A - Medicinal plants from the Eastern Himalayas of Northeast India are integral to traditional therapies for inflammation. This study evaluated the anti-inflammatory potential of 23 reported phytochemicals isolated from this region by targeting TNF-α, a critical mediator of autoimmune disorders. TNF-α production was induced in human peripheral blood mononuclear cells using 100 ng/mL lipopolysaccharide for 24 h and quantified using ELISA. The IC was calculated by probit regression, with methotrexate as the reference standard (IC = 1.96 µM; 94% inhibition at 3.5 µM). Rutin emerged as the most potent inhibitor (IC = 2.15 µM; 93% inhibition at 4 µM), followed by myricitrin and quercetin-3-O-glucoside (IC < 10 µM). Conversely, anthocyanins and punicic acid were the least effective (> 300 µM). QSAR modelling indicated that bioactivity correlates with small, planar, polyhydroxylated structures, specifically 3-O-glycosylated flavonols and ellagitannin-like polyphenols, whereas steric bulk in larger molecules diminished efficacy. Molecular docking against TNF-α (PDB: 2AZ5; Glide XP) revealed strong binding affinities for rutin (- 10.352 kcal/mol), myricitrin (- 10.020 kcal/mol), and quercetin-3-O-glucoside (- 10.011 kcal/mol), driven by hydrogen bonding (TYR151, SER60, LEU120, and GLY121) and π-π stacking (TYR119). However, 100 ns Molecular Dynamics simulations and MM-GBSA analyses identified myricitrin as the most thermodynamically stable complex, characterized by tight pocket occupancy, sustained hydrogen bonding, and reduced RMSD fluctuations. Taken together, these results imply that rutin and myricitrin are promising scaffolds for the development of anti-inflammatory drugs. - Source: PubMed
Publication date: 2026/05/08
Ray Sarbani DeyRay SupratimPandit BibhasGhosh SubhamMurmu AnjaliRoy Partha PratimSingh Jagadish